>YOR260W GCD1 SGDID:S000005786, Chr XV from 813984-815720, Verified
           ORF, "Gamma subunit of the translation initiation factor
           eIF2B, the guanine-nucleotide exchange factor for eIF2;
           activity subsequently regulated by phosphorylated eIF2;
           first identified as a negative regulator of GCN4
           expression"
          Length = 578

 Score = 46.6 bits (109), Expect = 2e-09
 Identities = 86/474 (18%), Positives = 183/474 (38%), Gaps = 56/474 (11%)

Query: 39  KALIKICNRCMIYYIIKNIIEQRLKYITIV------------------VNSKYYDDMVNY 80
           KAL+ I NR MI Y++    +   K I++V                  +  + ++ +   
Sbjct: 66  KALLPIGNRPMIEYVLDWCDQADFKEISVVAPVDEIELIESGLTSFLSLRKQQFELIYKA 125

Query: 81  INTTFQDNYKYDDKKGKHIYCIDIEPYNTNNNEDIGSIQCLLQIKNKIKSDFIVVNCDIL 140
           ++ +   ++  D KK      I+  P   N+  +  S+Q   ++  +I  DF+++ CD +
Sbjct: 126 LSNSNHSHHLQDPKK------INFIPSKANSTGE--SLQ--KELLPRINGDFVILPCDFV 175

Query: 141 GFVDFHSLANLFRG--ENAICAILLLENNQPSNDKKKKEITDEYVNLENNVWVCIDKNSK 198
             +    L + FR   +N +   +  +N+  S+  KK++   +       V+   + + +
Sbjct: 176 TDIPPQVLVDQFRNRDDNNLAMTIYYKNSLDSSIDKKQQQKQKQQQF-FTVYSENEDSER 234

Query: 199 VVSIKDSLSMKENGKMKISKVN---LLFHKNFVLKTDLLDSHVYIFKHYVLEIMEQKKNK 255
              + D  S ++  K K  ++    L  + N  + T LL+S +Y     + ++++     
Sbjct: 235 QPILLDVYSQRDVTKTKYLQIRSHLLWNYPNLTVSTKLLNSFIYFCSFELCQLLKLGPQS 294

Query: 256 FSSIKYDLIPYLVKIQNTSKAAEYSKGEFKYNMYNTLIEKYEGDDEIEEGKRENLMLDII 315
            S       P+    Q  +        +  ++  +               K+ +L+LD I
Sbjct: 295 MSRQASFKDPFTGNQQQQNPPTTDDDEDRNHDDDDDYKPSATSIQPTYFKKKNDLILDPI 354

Query: 316 N-NENVESVVCYIQPKNNGFCQRINSIPNFFKANLLFCVSRQDHLKNILPPYCFFLLTEK 374
           N N+++  V   +  ++    +    I  F   N    + R ++L   +    F L  + 
Sbjct: 355 NCNKSLSKVFRDLSRRSWQHSKPREPIGIFILPNETLFI-RANNLNAYMDANRFVLKIKS 413

Query: 375 NQSF------------KDCIISSHFDHEENILLKKSILGKNVTIKKNSSINRSILMDNIT 422
              F             D I+        +  +K S+LG    I     +  S+L   + 
Sbjct: 414 QTMFTKNIQIQSAAIGADAIVDPKCQISAHSNVKMSVLGTQANIGSRCRVAGSLLFPGVH 473

Query: 423 IHEKCVIQNSIICDNVVIEENCKSSYYNTLYLIDCIIKENSVIQKNSVHEKETL 476
           + ++ +++N II     I   CK        L +C I+ + V++  +  + ETL
Sbjct: 474 LGDEVILENCIIGPMAKIGSKCK--------LSNCYIEGHYVVEPKNNFKGETL 519

 Score = 16.9 bits (32), Expect = 1.9
 Identities = 4/12 (33%), Positives = 9/12 (75%)

Query: 82  NTTFQDNYKYDD 93
           ++ + D Y+Y+D
Sbjct: 561 DSEYTDEYEYED 572

 Score = 16.5 bits (31), Expect = 2.5
 Identities = 18/64 (28%), Positives = 26/64 (40%), Gaps = 8/64 (12%)

Query: 120 CLLQIKNKIKSDFIVVNCDILGFVDFHSLANLFRGENAICAILLLENNQPSNDKKKKEIT 179
           C++    KI S   + NC I G        N F+GE        L N     D++ + I 
Sbjct: 483 CIIGPMAKIGSKCKLSNCYIEGHYVVEP-KNNFKGET-------LANVYLDEDEEDELIY 534

Query: 180 DEYV 183
           D+ V
Sbjct: 535 DDSV 538

Lambda     K      H
   0.320    0.137    0.399 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 743
Number of extensions: 55
Number of successful extensions: 11
Number of sequences better than 10.0: 1
Number of HSP's gapped: 8
Number of HSP's successfully gapped: 8
Length of query: 481
Length of database: 578
Length adjustment: 35
Effective length of query: 446
Effective length of database: 543
Effective search space:   242178
Effective search space used:   242178
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 26 (14.9 bits)
S2: 26 (14.6 bits)