>YBL024W NCL1 SGDID:S000000120, Chr II from 172537-174591, Verified
           ORF, "S-adenosyl-L-methionine-dependent tRNA:
           m5C-methyltransferase, methylates cytosine to m5C at
           several positions in tRNAs and intron-containing
           pre-tRNAs; similar to Nop2p and human proliferation
           associated nucleolar protein p120"
          Length = 684

 Score =  159 bits (402), Expect = 7e-43
 Identities = 102/271 (37%), Positives = 134/271 (49%), Gaps = 49/271 (18%)

Query: 337 NNPDGVVVANDSNFKRCCMLFHRLKNIHSDCLVVTNNNAVTFPYIYIKRXXXXXXXXXXX 396
           + P G VVAND++ +R  ML H+LK ++S  L+V N++A  FP I +             
Sbjct: 192 DEPSGFVVANDADARRSHMLVHQLKRLNSANLMVVNHDAQFFPRIRLHGNS--------- 242

Query: 397 XXXXXXXXXXXXXXXILQNENSIFVKKNFDSILCDVPCSGDGTLRKDRNVWINWNPNNAY 456
                             N+N +     FD ILCDVPCSGDGT+RK+ NVW +WN     
Sbjct: 243 -----------------NNKNDVL---KFDRILCDVPCSGDGTMRKNVNVWKDWNTQAGL 282

Query: 457 NLFQMQVNILKRSIELTKENGYIVYSTCSLNPIENEAVICEIFNSVENLDCLKLINFQNE 516
            L  +Q+NIL R + L K NG +VYSTCSLNPIENEAV+ E        D ++L+N  ++
Sbjct: 283 GLHAVQLNILNRGLHLLKNNGRLVYSTCSLNPIENEAVVAEALRKWG--DKIRLVNCDDK 340

Query: 517 LLTKLNYEKAVKQWKVVMDDIWFDTYEQYCSYLQNKESGKYKKIYEKIQEGMFPPCEEFI 576
            L  L   K V +W V            Y   L  K  G      E   +  F P EE  
Sbjct: 341 -LPGLIRSKGVSKWPV------------YDRNLTEKTKGD-----EGTLDSFFSPSEEEA 382

Query: 577 NSINLNYAKRFFPHHYNAGGFFIAVIQKGEN 607
           +  NL    R +PH  N GGFFI V +K E+
Sbjct: 383 SKFNLQNCMRVYPHQQNTGGFFITVFEKVED 413

 Score = 72.4 bits (176), Expect = 1e-16
 Identities = 44/158 (27%), Positives = 82/158 (51%), Gaps = 9/158 (5%)

Query: 16  LEKNESFFNYYINQKILKSCEIDNFMEIINKELPITFRVLNNNKYSNFIHENIKKQ-LES 74
           +++NE +  YY    +    + + F +     LP+TFR+  + K++  +    K++ L +
Sbjct: 31  VKENEKWEKYYKTLALFPEDQWEEFKKTCQAPLPLTFRITGSRKHAGEVLNLFKERHLPN 90

Query: 75  ICKDKYSIIKLNEE-------DHM-YEIHLTRSEIKKEESXXXXXXXXXXXXESGFIFRQ 126
           +   ++   K+          DH+ +++ + ++ I+K E               G I RQ
Sbjct: 91  LTNVEFEGEKIKAPVELPWYPDHLAWQLDVPKTVIRKNEQFAKTQRFLVVENAVGNISRQ 150

Query: 127 ELVSMLPVLFLRLKENFFVLDICAAPGSKTAQILDYMH 164
           E VSM+P + L +K +  VLD+CAAPGSKTAQ+++ +H
Sbjct: 151 EAVSMIPPIVLEVKPHHTVLDMCAAPGSKTAQLIEALH 188

 Score = 21.9 bits (45), Expect = 0.18
 Identities = 38/190 (20%), Positives = 71/190 (37%), Gaps = 37/190 (19%)

Query: 834  LNMNFLTIKNN---LYIHLKDNGNTHKLSVDERINSNVKKIYLVSNSAK--EILESYTKM 888
            LN     +KNN   +Y     N   ++  V E +     KI LV+   K   ++ S    
Sbjct: 292  LNRGLHLLKNNGRLVYSTCSLNPIENEAVVAEALRKWGDKIRLVNCDDKLPGLIRSKGVS 351

Query: 889  KLKIITAGITVIQVDKNKKNTYENYY--------RINYSGCLNFIPFFQDIDNFFKNKKY 940
            K  +    +T  +  K  + T ++++        + N   C+   P  Q+   F      
Sbjct: 352  KWPVYDRNLT--EKTKGDEGTLDSFFSPSEEEASKFNLQNCMRVYPHQQNTGGF------ 403

Query: 941  REQIIQNYFYTVYENKERQFNFDKYMNQLIDERKKTNLSKLDSNKNKAQKILTSELKNEK 1000
                    F TV+E  E          +   E+  +    L+S   + +KI   E++ +K
Sbjct: 404  --------FITVFEKVEDS-------TEAATEKLSSETPALESEGPQTKKIKVEEVQ-KK 447

Query: 1001 DNIENNMSEE 1010
            + +  + +EE
Sbjct: 448  ERLPRDANEE 457

 Score = 16.5 bits (31), Expect = 7.7
 Identities = 8/39 (20%), Positives = 19/39 (48%)

Query: 278 DCFKNENITNEYKKDEYKENIYKTFYNILENNNYDDPFF 316
           D  +++NI N++ +   K +    F ++  N+   +  F
Sbjct: 578 DDIRSKNIDNDFVEKMTKLSSGCAFIDVSRNDPAKENLF 616

 Score = 16.5 bits (31), Expect = 7.7
 Identities = 6/11 (54%), Positives = 9/11 (81%)

Query: 906 KKNTYENYYRI 916
           K++T+E  YRI
Sbjct: 632 KEDTHELLYRI 642

Lambda     K      H
   0.316    0.134    0.384 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1888
Number of extensions: 89
Number of successful extensions: 8
Number of sequences better than 10.0: 1
Number of HSP's gapped: 6
Number of HSP's successfully gapped: 6
Length of query: 1193
Length of database: 684
Length adjustment: 43
Effective length of query: 1150
Effective length of database: 641
Effective search space:   737150
Effective search space used:   737150
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 31 (17.0 bits)
S2: 31 (16.5 bits)