>YEL031W SPF1 SGDID:S000000757, Chr V from 90258-93905, Verified ORF,
            "P-type ATPase, ion transporter of the ER membrane
            involved in ER function and Ca2+ homeostasis; required
            for regulating Hmg2p degradation; confers sensitivity to
            a killer toxin (SMKT) produced by Pichia farinosa KK1"
          Length = 1215

 Score =  125 bits (315), Expect = 3e-32
 Identities = 75/248 (30%), Positives = 127/248 (51%), Gaps = 7/248 (2%)

Query: 758  NRLDIKNKHKKHIVYAGTNILMTKNENNKFNGKKLPVNGCIGIVLRSGFSTYQGKLVRTI 817
            + L +    K  +++ GT  L      +K +    P  G + IV ++GF T QG LVR +
Sbjct: 326  DNLQLDGVDKIAVLHGGTKALQVTPPEHKSDIPPPPDGGALAIVTKTGFETSQGSLVRVM 385

Query: 818  INTSEKVNXXXXXXXXXXXXXXXXXXXXXAYVVYSVLKTNEERNLYKLLLSVSHIITAVI 877
            I ++E+V+                      YV     K    ++  KL+L    IIT+V+
Sbjct: 386  IYSAERVSVDNKEALMFILFLLIFAVIASWYVWVEGTKMGRIQS--KLILDCILIITSVV 443

Query: 878  PPEFPITLSLAVTISIVYLYNMKIYCTEPFRLPFSGKTNICAFDKTGTLTEDNMIVLGLF 937
            PPE P+ L++AV  S+  L    +YCTEPFR+PF+G+ ++C FDKTGTLT ++++  GL 
Sbjct: 444  PPELPMELTMAVNSSLAALAKFYVYCTEPFRIPFAGRIDVCCFDKTGTLTGEDLVFEGLA 503

Query: 938  GLDDNLKRINEINESIINKQKIPFFSLSVIAGCHSICTL-NNKLLGDPLEKNSFLKLKCV 996
            G+  + + I      + +  + P  ++ VI   H++  L +  ++GDP+EK +   +   
Sbjct: 504  GISADSENIRH----LYSAAEAPESTILVIGAAHALVKLEDGDIVGDPMEKATLKAVGWA 559

Query: 997  MKSLNHTY 1004
            ++  N  Y
Sbjct: 560  VERKNSNY 567

 Score =  115 bits (287), Expect = 5e-29
 Identities = 65/191 (34%), Positives = 99/191 (51%)

Query: 1657 DSLPLIKLGEASIASPFTYKGNDIKCVKEIISCGRCALSKVIMMYKLMIINSLITAFSVS 1716
            D  P +KLG+AS A+PFT K  ++  V  II  GRCAL   I MYK++ +N LI+A+S+S
Sbjct: 948  DEAPALKLGDASCAAPFTSKLANVSAVTNIIRQGRCALVNTIQMYKILALNCLISAYSLS 1007

Query: 1717 ILTLDGVKLSDAQTTIISLLYTCLIVLISKTSPLKNITNYSPPNSLFNFSVXXXXXXXXX 1776
            I+ + GVK  D Q T+  LL +   + IS+  PL+ ++   P + +FN  +         
Sbjct: 1008 IIYMAGVKFGDGQATVSGLLLSVCFLSISRGKPLEKLSKQRPQSGIFNVYIMGSILSQFA 1067

Query: 1777 XXXXXXXYGWKLACVYREINYIPDIKGDFIPNLVNTCIYYLIYCINLSIFSCNYEGLPFM 1836
                   Y               D++ +F P+L+NT I+ +     +S F+ NY+G PF 
Sbjct: 1068 VHIATLVYITTEIYKLEPREPQVDLEKEFAPSLLNTGIFIIQLVQQVSTFAVNYQGEPFR 1127

Query: 1837 VPIHKNKEIVY 1847
              I  NK + Y
Sbjct: 1128 ENIRSNKGMYY 1138

 Score =  111 bits (277), Expect = 7e-28
 Identities = 53/146 (36%), Positives = 84/146 (57%), Gaps = 3/146 (2%)

Query: 498 YGENIYDIPCPCFKELLYESMLSPFFIFQFFSIVLWMLDSYWYFGXXXXXXXXXXXXXXX 557
           YGEN +DIP P F EL  E  ++P F+FQ F + LW+LD +WY+                
Sbjct: 177 YGENSFDIPIPTFMELFKEHAVAPLFVFQVFCVALWLLDEFWYYSLFNLFMIISMEAAAV 236

Query: 558 NKRIREFNMINGMKVDPQDVYVYRNLRWTIMKSNKLLPGDIYILTNDMTATDNNICTCET 617
            +R+        M + P  + V+RN +W  +++N+LLP D+  +T   TA ++ I  C+ 
Sbjct: 237 FQRLTALKEFRTMGIKPYTINVFRNKKWVALQTNELLPMDLVSITR--TAEESAI-PCDL 293

Query: 618 LLIDGTCITDESILTGESVPLIKACI 643
           +L+DG+ I +E++L+GES PL+K  I
Sbjct: 294 ILLDGSAIVNEAMLSGESTPLLKESI 319

 Score = 81.6 bits (200), Expect = 6e-19
 Identities = 44/111 (39%), Positives = 67/111 (60%), Gaps = 2/111 (1%)

Query: 1229 KGSPEIMKRFLKKVPEHYDEVLNSLSIKGYRVLCLAVNILDNNMYK--ENISREEVEKDL 1286
            KG+PE ++  L  +P++YDE+  S +  G RVL LA   L        ++++R++VE +L
Sbjct: 604  KGAPETIRERLSDIPKNYDEIYKSFTRSGSRVLALASKSLPKMSQSKIDDLNRDDVESEL 663

Query: 1287 YFCGFLTFICPIKVTTPNYILHIKNAGIKNIMITGDNALTACQVSQDVNIV 1337
             F GFL F CP+K      I  +  +  ++IMITGDN LTA  V+++V IV
Sbjct: 664  TFNGFLIFHCPLKDDAIETIKMLNESSHRSIMITGDNPLTAVHVAKEVGIV 714

 Score = 70.5 bits (171), Expect = 1e-15
 Identities = 33/80 (41%), Positives = 52/80 (65%), Gaps = 5/80 (6%)

Query: 1481 FSLCITGDIIDYFLEVYKNNLHIFNELIRGVHIFCRMSPKNKEIIIKTLNKIGYITIMCG 1540
            + + +TG    Y L   + +  +  +L+R   ++ R+SP  KE ++ TL  +GY T+MCG
Sbjct: 761  YDIAVTG----YALNALEGHSQL-RDLLRHTWVYARVSPSQKEFLLNTLKDMGYQTLMCG 815

Query: 1541 DGTNDMAALKAAHVGVSLLS 1560
            DGTND+ ALK AHVG++LL+
Sbjct: 816  DGTNDVGALKQAHVGIALLN 835

Lambda     K      H
   0.322    0.139    0.415 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 3961
Number of extensions: 166
Number of successful extensions: 12
Number of sequences better than 10.0: 1
Number of HSP's gapped: 6
Number of HSP's successfully gapped: 6
Length of query: 1918
Length of database: 1215
Length adjustment: 51
Effective length of query: 1867
Effective length of database: 1164
Effective search space:  2173188
Effective search space used:  2173188
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 35 (19.1 bits)
S2: 35 (18.1 bits)