>YPL212C PUS1 SGDID:S000006133, Chr XVI from 153148-151514, reverse
           complement, Verified ORF, "tRNA:pseudouridine synthase,
           introduces pseudouridines at positions 26-28, 34-36, 65,
           and 67 of tRNA; nuclear protein that appears to be
           involved in tRNA export; also acts on U2 snRNA"
          Length = 544

 Score = 85.1 bits (209), Expect = 6e-21
 Identities = 76/312 (24%), Positives = 143/312 (45%), Gaps = 26/312 (8%)

Query: 225 DHLPCDIKCFKIYKVTKSFDARKFCSFRFYEYLFPVYVLSEVQVNEKYKETFHQAILDID 284
           + LP  I+ + I +V K+FD RK CS R+YEYL P Y L   +          ++  ++ 
Sbjct: 161 EKLPEGIRVWDIERVNKAFDCRKMCSSRWYEYLLPTYSLIGPKPGSILYRDIEESKTELP 220

Query: 285 EYVQRCKEEKRTRRNNEKNHIDSTNKENENIYFKNGDNIDSMNSTTNNNSKDFSCTKDNT 344
             +    E K      +K+  +  + E           I+++ +       +F   ++  
Sbjct: 221 GVLDEDLESKEFWEEFKKDANEKFSTEE----------IEAILAYVPPARDEFDINEE-- 268

Query: 345 EQPNIVENIKRDIIDEDIFSIKHFKEDLNEEELNTFFDIFNNYVGYHNFHCFTKKKI--D 402
               + + +K+    + + +    +  ++  +L  F    + Y+G HNFH FT  K   +
Sbjct: 269 ----LYQKVKKY---KQLENAHRRRYRISAAKLAKFRASTSQYLGAHNFHNFTLGKDFKE 321

Query: 403 QTTYRFIKYFDVSTVKLF---DYHFLSVKILGQSFLMHQIRKMITLAVETYRKATSINSI 459
            +  RF+K   VS   +       ++S+KI GQSF++HQIRKM+++A    R    +  I
Sbjct: 322 PSAIRFMKDIKVSDPFVIGDAQTEWISIKIHGQSFMLHQIRKMVSMATLITRCGCPVERI 381

Query: 460 YYCLNTKNYIPITLFPSDGLMLICPYFNAYNEKVCKQPHTLPIYFEETEDIMEFK-KNKI 518
                 +  I I   P+ GL+L  P F  YN+++ +  +    + +  +++ +FK K+  
Sbjct: 382 SQAYGQQK-INIPKAPALGLLLEAPVFEGYNKRLEQFGYKAIDFSKYQDEVDKFKMKHIY 440

Query: 519 GKCIIEKMKQNV 530
            K   E++ +NV
Sbjct: 441 DKIYKEEVDENV 452

 Score = 43.5 bits (101), Expect = 2e-08
 Identities = 27/82 (32%), Positives = 41/82 (50%), Gaps = 1/82 (1%)

Query: 54  ALCIGYIGSQYHGCQGQGENCETIENTLERTLLKINAIXXXXXXXXXXXCLSRSARTDQG 113
           A+ +GY G+ YHG Q    N  TIE+ L +  ++  AI              R+ARTD+G
Sbjct: 78  AVMVGYCGTGYHGMQYNPPN-PTIESALFKAFVEAGAISKDNSNDLKKNGFMRAARTDKG 136

Query: 114 VHALFNIFVYNVDINCTNMKNE 135
           VHA  N+    + I   ++K +
Sbjct: 137 VHAGGNLISLKMIIEDPDIKQK 158

 Score = 24.6 bits (52), Expect = 0.010
 Identities = 14/37 (37%), Positives = 19/37 (51%), Gaps = 8/37 (21%)

Query: 361 DIFSIKH-----FKEDLNEEELNTFF---DIFNNYVG 389
           D F +KH     +KE+++E   N FF   D FN   G
Sbjct: 432 DKFKMKHIYDKIYKEEVDENVFNAFFSYIDSFNKVTG 468

 Score = 21.2 bits (43), Expect = 0.11
 Identities = 24/95 (25%), Positives = 36/95 (37%), Gaps = 11/95 (11%)

Query: 262 VLSEVQVNEKYK---ETFHQAILDIDEYVQRCKEEKRTRRNNEKNHIDSTNKE--NENIY 316
           +L E  V E Y    E F    +D  +Y     + K       K+  D   KE  +EN++
Sbjct: 400 LLLEAPVFEGYNKRLEQFGYKAIDFSKYQDEVDKFKM------KHIYDKIYKEEVDENVF 453

Query: 317 FKNGDNIDSMNSTTNNNSKDFSCTKDNTEQPNIVE 351
                 IDS N  T    ++ +       Q +I E
Sbjct: 454 NAFFSYIDSFNKVTGAQGEETADKSGPAVQKSIFE 488

 Score = 18.5 bits (36), Expect = 0.69
 Identities = 8/24 (33%), Positives = 14/24 (58%), Gaps = 1/24 (4%)

Query: 292 EEKRTRRNNEKNHIDSTNKENENI 315
           +EK  +++N+K HID   K    +
Sbjct: 37  DEKDKKKDNDK-HIDKRPKSGPRL 59

 Score = 16.2 bits (30), Expect = 3.4
 Identities = 6/12 (50%), Positives = 8/12 (66%)

Query: 309 NKENENIYFKNG 320
           +K+N N   KNG
Sbjct: 115 SKDNSNDLKKNG 126

 Score = 15.0 bits (27), Expect = 7.6
 Identities = 5/18 (27%), Positives = 12/18 (66%)

Query: 10  NNYEKNKERIKKLKLERQ 27
           + ++ N+E  +K+K  +Q
Sbjct: 261 DEFDINEELYQKVKKYKQ 278

Lambda     K      H
   0.320    0.136    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 642
Number of extensions: 37
Number of successful extensions: 15
Number of sequences better than 10.0: 1
Number of HSP's gapped: 9
Number of HSP's successfully gapped: 9
Length of query: 530
Length of database: 544
Length adjustment: 35
Effective length of query: 495
Effective length of database: 509
Effective search space:   251955
Effective search space used:   251955
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 26 (14.9 bits)
S2: 26 (14.6 bits)