>YLR212C TUB4 SGDID:S000004202, Chr XII from 566283-564862, reverse
           complement, Verified ORF, "Gamma-tubulin, involved in
           nucleating microtubules from both the cytoplasmic and
           nuclear faces of the spindle pole body"
          Length = 473

 Score =  306 bits (784), Expect = 9e-88
 Identities = 166/459 (36%), Positives = 273/459 (59%), Gaps = 15/459 (3%)

Query: 1   MPREIITLQCGQCGNQIGVEFWKQLCNEHNIDQEGI--LKNNNFLNEDRKDIFFYQADDE 58
           M  EIITLQ GQCGN +G   W QL  EH I  +G+  L +++   +D    FF +    
Sbjct: 1   MGGEIITLQAGQCGNHVGKFLWSQLAKEHAIGTDGLSQLPDSSTERDDDTKPFFRENSRN 60

Query: 59  HFIPRALLFDLEPRVINSIQTSEYRNLYNPENMFISKEXXXXXXXXXXXYSQGHKVEEEI 118
            F PRA++ D EP VI  ++ + +R  ++P N +++ +           Y  G + +++I
Sbjct: 61  KFTPRAIMMDSEPSVIADVENT-FRGFFDPRNTWVASDGASAGNSWANGYDIGTRNQDDI 119

Query: 119 IDMIDREVDNSDNLEGFILSHSIAGGTGSGMGSYLLELLNDNYSKKMIQTFSVFPLLTNE 178
           ++ ID+E+D++DN EGF L HS+AGGTGSG+GS LLE L D Y KK++ T+SVFP     
Sbjct: 120 LNKIDKEIDSTDNFEGFQLLHSVAGGTGSGLGSNLLEALCDRYPKKILTTYSVFPA---R 176

Query: 179 SSDVVVQPYNSILTLKRLILSTDSVVVIDNTSLNRIFVERLKLNNPTFQQTNTIISNVMS 238
           SS+VVVQ YN+IL L+RLI  +D+ VV DN SL  I  +  +  N   Q TN +IS ++S
Sbjct: 177 SSEVVVQSYNTILALRRLIEDSDATVVFDNASLLNISGKVFRNPNIDLQHTNQLISTIIS 236

Query: 239 ASTTTLRYPGSMNNDMISLISSLIINPKCHFLITSYTPITIDKHISNV--QKTTVLDVMK 296
           + T ++R+P  M + M S+ S+LI +P+ HFL  S+TP T D    ++  +  +  DVM 
Sbjct: 237 SVTNSIRFPSYMYSSMSSIYSTLIPSPELHFLSPSFTPFTSDYIHDDIAHKGHSSYDVML 296

Query: 297 RLLHTKNIMVSAPVRRGMYISILNIIRGETDPTQVHKGLQRIRDRKLVNFIKWNPASIQV 356
            LL   N +VS  +    Y ++ N I G  +P Q+ + + +++ R  + F  W+ +++ V
Sbjct: 297 DLLDPSNSLVSTAMNNPTYFNVYNTIIGNVEPRQISRAMTKLQQR--IKFPSWSSSAMHV 354

Query: 357 TLAKQSPHV---VSQHKVCGLMMANHTSISTLFERCVTQFDRLYKRRAFLENYKKESMFS 413
            + ++SP++    ++++V G+M++N +++  +FE     FD+++ + AFL NY    +F 
Sbjct: 355 NIGRRSPYLPLQPNENEVSGMMLSNMSTVVNVFENACNTFDKVFAKGAFLNNYNVGDLFQ 414

Query: 414 SADGQGNFEEMESSKEITQNLIDEYKSAERDDYFTNTYI 452
           S   Q   +E   S+E+ Q+L+++Y +AE+D Y  +  +
Sbjct: 415 SM--QNVQDEFAESREVVQSLMEDYVAAEQDSYLDDVLV 451

Lambda     K      H
   0.319    0.134    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 550
Number of extensions: 38
Number of successful extensions: 13
Number of sequences better than 10.0: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 452
Length of database: 473
Length adjustment: 33
Effective length of query: 419
Effective length of database: 440
Effective search space:   184360
Effective search space used:   184360
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 25 (14.4 bits)
S2: 25 (14.2 bits)