>YLR172C DPH5 SGDID:S000004162, Chr XII from 502164-501262, reverse
           complement, Verified ORF, "Methyltransferase required
           for synthesis of diphthamide, which is a modified
           histidine residue of translation elongation factor 2
           (Eft1p or Eft2p); not essential for viability; GFP-Dph5p
           fusion protein localizes to the cytoplasm"
          Length = 300

 Score =  251 bits (642), Expect = 1e-71
 Identities = 130/263 (49%), Positives = 177/263 (67%), Gaps = 1/263 (0%)

Query: 2   VLYIIGLGLGDEKDITIKGKELIEKSDVVYLETYTSILFV-SKDVLEETYKKSIEEVDRD 60
           +LY+IGLGL  + DIT++G E I+K   VYLE YTSIL   S++ LE  Y K I   DR+
Sbjct: 1   MLYLIGLGLSYKSDITVRGLEAIKKCSRVYLEHYTSILMAASQEELESYYGKEIILADRE 60

Query: 61  FAEENCDKILDEAKNKKVSFLVVGDPLCATTHHDIILRAKKKNIDVEIIHNTSIISAIGE 120
             E    +IL+ A  + V+FLVVGDP  ATTH D++LRAK++ I VEIIHN S+++A+G 
Sbjct: 61  LVETGSKQILNNADKEDVAFLVVGDPFGATTHTDLVLRAKREAIPVEIIHNASVMNAVGA 120

Query: 121 CGMQLYNFGQIVSIPYFEDNYKPTSYYDKIYINLKNNFHTLCLLDIKVKERTVENIMRNK 180
           CG+QLYNFGQ VS+ +F DN++P S+YDKI+ N K   HTL LLDIKVKE+++EN+ R +
Sbjct: 121 CGLQLYNFGQTVSMVFFTDNWRPDSWYDKIWENRKIGLHTLVLLDIKVKEQSIENMARGR 180

Query: 181 KIYEPPRFMTINDSIEQLLYCEHIHKKNIITKNTLGIAIIQIGTDNQQIISGDLLTLKDI 240
            IYEPPR+M+I    EQLL  E        T +T  +AI ++G+ +Q   SG +  L + 
Sbjct: 181 LIYEPPRYMSIAQCCEQLLEIEEKRGTKAYTPDTPAVAISRLGSSSQSFKSGTISELANY 240

Query: 241 SYNKPLHSLIICAPTLHDIEKEY 263
              +PLHSL+I     H++E EY
Sbjct: 241 DSGEPLHSLVILGRQCHELELEY 263

 Score = 14.6 bits (26), Expect = 2.7
 Identities = 7/30 (23%), Positives = 19/30 (63%), Gaps = 2/30 (6%)

Query: 169 KERTVENIMRNKKIYEPPRFM--TINDSIE 196
           KE+  +++  +++ ++P  ++  T +DS E
Sbjct: 271 KEKFGKDVANDQEYFKPAAWVPPTEDDSDE 300

Lambda     K      H
   0.320    0.139    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 266
Number of extensions: 10
Number of successful extensions: 4
Number of sequences better than 10.0: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 274
Length of database: 300
Length adjustment: 26
Effective length of query: 248
Effective length of database: 274
Effective search space:    67952
Effective search space used:    67952
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 22 (13.0 bits)
S2: 22 (13.1 bits)