Query= PF10_0314 Pf
Annotation|Plasmodium_falciparum_TIGR|(protein coding) asparagine-rich
antigen
(1190 letters)
>YDL035C GPR1 SGDID:S000002193, Chr IV from 392054-389169, reverse
complement, Verified ORF, "Plasma membrane G protein
coupled receptor (GPCR) that interacts with the
heterotrimeric G protein alpha subunit, Gpa2p, and with
Plc1p; sensor that integrates nutritional signals with
the modulation of cell fate via PKA and cAMP synthesis"
Length = 961
Score = 39.3 bits (90), Expect = 2e-06
Identities = 29/111 (26%), Positives = 47/111 (42%), Gaps = 15/111 (13%)
Query: 120 SCNNKYNKNGFVYDYNTYYTKKSSDLKGKLKNENESMNSDGNNDVTRINSNHSNNIVXXX 179
SC N +YD +SD+ +K + +N++ NND N+N+ N+
Sbjct: 458 SCQNSLESPLAMYDNKN----DNSDITSNIKEKGGIINNNSNNDDDDNNNNNDND----- 508
Query: 180 XXXXXXXXXIPKDNNISHVNNMTPINNMMKGINNRNDSVNTHMYNMKNLKD 230
+NN ++ NN NN NN N++ N + N+KN D
Sbjct: 509 ------NDNNNSNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNSNNIKNNVD 553
Score = 35.0 bits (79), Expect = 3e-05
Identities = 24/106 (22%), Positives = 44/106 (41%), Gaps = 18/106 (16%)
Query: 122 NNKYNKNGFVYDYNTYYTKKSSDLKGKLKNENESMNSDGNNDVTRINSNHSNNIVXXXXX 181
+N K G + N D N+N++ NS+ NN+ N+N++NN
Sbjct: 481 SNIKEKGGII---NNNSNNDDDDNNNNNDNDNDNNNSNNNNNNNNNNNNNNNN------- 530
Query: 182 XXXXXXXIPKDNNISHVNNMTPINNMMKGINNRNDSVNTHMYNMKN 227
+NN ++ NN NN+ ++N N + ++ + N
Sbjct: 531 --------NNNNNNNNNNNNNNSNNIKNNVDNNNTNPADNIPTLSN 568
Score = 32.3 bits (72), Expect = 2e-04
Identities = 15/45 (33%), Positives = 25/45 (55%)
Query: 768 HDKNTEQNIMLNDELNNNKSPNILNKSYIDNNKNNGSHNEINSKK 812
+D + + N N+ NNN + N N + +NN NN ++N N+ K
Sbjct: 505 NDNDNDNNNSNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNSNNIK 549
Score = 32.0 bits (71), Expect = 3e-04
Identities = 16/43 (37%), Positives = 23/43 (53%)
Query: 768 HDKNTEQNIMLNDELNNNKSPNILNKSYIDNNKNNGSHNEINS 810
+D N N N+ NNN + N N + +NN NN S+N N+
Sbjct: 509 NDNNNSNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNSNNIKNN 551
Score = 30.8 bits (68), Expect = 6e-04
Identities = 18/67 (26%), Positives = 30/67 (44%)
Query: 768 HDKNTEQNIMLNDELNNNKSPNILNKSYIDNNKNNGSHNEINSKKGGEAIMSLLGLSKNV 827
+D + N ND NNN + N N + +NN NN ++N N+ ++ N
Sbjct: 496 NDDDDNNNNNDNDNDNNNSNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNSNNIKNNVDNN 555
Query: 828 DTKLEDD 834
+T D+
Sbjct: 556 NTNPADN 562
Score = 29.6 bits (65), Expect = 0.001
Identities = 20/69 (28%), Positives = 33/69 (47%), Gaps = 8/69 (11%)
Query: 742 NINVSTKKDEKAGRKLLYLIKGLSMVHDKNTEQNIMLNDELNNNKSPNILNKSYIDNNKN 801
N ++++ EK G +I S D + N ND N+N + N N + +NN N
Sbjct: 476 NSDITSNIKEKGG-----IINNNSNNDDDDNNNN---NDNDNDNNNSNNNNNNNNNNNNN 527
Query: 802 NGSHNEINS 810
N ++N N+
Sbjct: 528 NNNNNNNNN 536
Score = 27.3 bits (59), Expect = 0.006
Identities = 20/73 (27%), Positives = 30/73 (41%), Gaps = 4/73 (5%)
Query: 742 NINVSTKKDEKAGRKLLY----LIKGLSMVHDKNTEQNIMLNDELNNNKSPNILNKSYID 797
N + T+K G + L L+M +KN +I N + N N D
Sbjct: 441 NEGLDTRKSSMLGHQTFSCQNSLESPLAMYDNKNDNSDITSNIKEKGGIINNNSNNDDDD 500
Query: 798 NNKNNGSHNEINS 810
NN NN + N+ N+
Sbjct: 501 NNNNNDNDNDNNN 513
Score = 26.2 bits (56), Expect = 0.014
Identities = 12/29 (41%), Positives = 18/29 (62%)
Query: 889 NNDHMIQNNDHMVDSNNNYYISHNNVDKN 917
NN++ NN++ ++NNN NNVD N
Sbjct: 527 NNNNNNNNNNNNNNNNNNSNNIKNNVDNN 555
Score = 25.8 bits (55), Expect = 0.018
Identities = 11/30 (36%), Positives = 19/30 (63%)
Query: 889 NNDHMIQNNDHMVDSNNNYYISHNNVDKNV 918
NN++ NN++ ++NNN + NN+ NV
Sbjct: 523 NNNNNNNNNNNNNNNNNNNNNNSNNIKNNV 552
Score = 24.6 bits (52), Expect = 0.040
Identities = 11/33 (33%), Positives = 21/33 (63%)
Query: 889 NNDHMIQNNDHMVDSNNNYYISHNNVDKNVGDI 921
NN++ NN++ ++NNN ++NN + N +I
Sbjct: 516 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNSNNI 548
Score = 24.3 bits (51), Expect = 0.052
Identities = 11/29 (37%), Positives = 18/29 (62%)
Query: 889 NNDHMIQNNDHMVDSNNNYYISHNNVDKN 917
NND+ NN+ ++NNN ++NN + N
Sbjct: 504 NNDNDNDNNNSNNNNNNNNNNNNNNNNNN 532
Score = 23.9 bits (50), Expect = 0.068
Identities = 11/29 (37%), Positives = 18/29 (62%)
Query: 889 NNDHMIQNNDHMVDSNNNYYISHNNVDKN 917
NND NN++ D++NN ++NN + N
Sbjct: 495 NNDDDDNNNNNDNDNDNNNSNNNNNNNNN 523
Score = 23.5 bits (49), Expect = 0.089
Identities = 10/29 (34%), Positives = 19/29 (65%)
Query: 889 NNDHMIQNNDHMVDSNNNYYISHNNVDKN 917
+ND+ NN++ ++NNN ++NN + N
Sbjct: 508 DNDNNNSNNNNNNNNNNNNNNNNNNNNNN 536
Score = 23.5 bits (49), Expect = 0.089
Identities = 10/29 (34%), Positives = 19/29 (65%)
Query: 889 NNDHMIQNNDHMVDSNNNYYISHNNVDKN 917
NN++ NN++ ++NNN ++NN + N
Sbjct: 515 NNNNNNNNNNNNNNNNNNNNNNNNNNNNN 543
Score = 23.1 bits (48), Expect = 0.12
Identities = 10/29 (34%), Positives = 18/29 (62%)
Query: 889 NNDHMIQNNDHMVDSNNNYYISHNNVDKN 917
NN++ +ND+ +NNN ++NN + N
Sbjct: 501 NNNNNDNDNDNNNSNNNNNNNNNNNNNNN 529
Score = 23.1 bits (48), Expect = 0.12
Identities = 10/29 (34%), Positives = 18/29 (62%)
Query: 889 NNDHMIQNNDHMVDSNNNYYISHNNVDKN 917
NN + NN++ ++NNN ++NN + N
Sbjct: 512 NNSNNNNNNNNNNNNNNNNNNNNNNNNNN 540
Score = 22.7 bits (47), Expect = 0.15
Identities = 10/29 (34%), Positives = 18/29 (62%)
Query: 889 NNDHMIQNNDHMVDSNNNYYISHNNVDKN 917
NN+ NN++ ++NNN ++NN + N
Sbjct: 511 NNNSNNNNNNNNNNNNNNNNNNNNNNNNN 539
Score = 22.3 bits (46), Expect = 0.20
Identities = 10/29 (34%), Positives = 18/29 (62%)
Query: 889 NNDHMIQNNDHMVDSNNNYYISHNNVDKN 917
++D NND+ D+NN+ ++NN + N
Sbjct: 497 DDDDNNNNNDNDNDNNNSNNNNNNNNNNN 525
Score = 21.9 bits (45), Expect = 0.26
Identities = 9/29 (31%), Positives = 19/29 (65%)
Query: 889 NNDHMIQNNDHMVDSNNNYYISHNNVDKN 917
N+++ NN++ ++NNN ++NN + N
Sbjct: 509 NDNNNSNNNNNNNNNNNNNNNNNNNNNNN 537
Score = 21.9 bits (45), Expect = 0.26
Identities = 9/29 (31%), Positives = 19/29 (65%)
Query: 889 NNDHMIQNNDHMVDSNNNYYISHNNVDKN 917
+N++ NN++ ++NNN ++NN + N
Sbjct: 510 DNNNSNNNNNNNNNNNNNNNNNNNNNNNN 538
Score = 21.9 bits (45), Expect = 0.26
Identities = 10/29 (34%), Positives = 19/29 (65%)
Query: 889 NNDHMIQNNDHMVDSNNNYYISHNNVDKN 917
NN++ NN++ ++NNN +++N KN
Sbjct: 522 NNNNNNNNNNNNNNNNNNNNNNNSNNIKN 550
Score = 21.9 bits (45), Expect = 0.26
Identities = 12/34 (35%), Positives = 18/34 (52%)
Query: 126 NKNGFVYDYNTYYTKKSSDLKGKLKNENESMNSD 159
N + F Y N Y K S+D K +E ++ +SD
Sbjct: 768 NCDDFEYWENYYSAKDSNDNKRTESDETKTNSSD 801
Score = 21.6 bits (44), Expect = 0.34
Identities = 9/29 (31%), Positives = 19/29 (65%)
Query: 889 NNDHMIQNNDHMVDSNNNYYISHNNVDKN 917
NN++ N+++ ++NNN ++NN + N
Sbjct: 502 NNNNDNDNDNNNSNNNNNNNNNNNNNNNN 530
Score = 21.6 bits (44), Expect = 0.34
Identities = 9/29 (31%), Positives = 19/29 (65%)
Query: 889 NNDHMIQNNDHMVDSNNNYYISHNNVDKN 917
N+++ NN++ ++NNN ++NN + N
Sbjct: 513 NSNNNNNNNNNNNNNNNNNNNNNNNNNNN 541
Score = 21.6 bits (44), Expect = 0.34
Identities = 9/29 (31%), Positives = 19/29 (65%)
Query: 889 NNDHMIQNNDHMVDSNNNYYISHNNVDKN 917
+N++ NN++ ++NNN ++NN + N
Sbjct: 514 SNNNNNNNNNNNNNNNNNNNNNNNNNNNN 542
Score = 21.6 bits (44), Expect = 0.34
Identities = 9/23 (39%), Positives = 14/23 (60%)
Query: 1046 SRHNDNNVESNVLNPYNTNSYNV 1068
+ +N+NN +N N N NS N+
Sbjct: 526 NNNNNNNNNNNNNNNNNNNSNNI 548
Score = 21.2 bits (43), Expect = 0.44
Identities = 14/43 (32%), Positives = 23/43 (53%), Gaps = 3/43 (6%)
Query: 936 MYNNICKKKESGI-GNVQKYENDLHNNSSFKYSKNLNKYENDD 977
MY+N K S I N+++ ++NNS+ N N +ND+
Sbjct: 469 MYDN--KNDNSDITSNIKEKGGIINNNSNNDDDDNNNNNDNDN 509
Score = 21.2 bits (43), Expect = 0.44
Identities = 10/29 (34%), Positives = 16/29 (55%)
Query: 889 NNDHMIQNNDHMVDSNNNYYISHNNVDKN 917
N+D NN+ + NNN ++NN + N
Sbjct: 496 NDDDDNNNNNDNDNDNNNSNNNNNNNNNN 524
Score = 21.2 bits (43), Expect = 0.44
Identities = 9/29 (31%), Positives = 18/29 (62%)
Query: 889 NNDHMIQNNDHMVDSNNNYYISHNNVDKN 917
NN+ +N++ ++NNN ++NN + N
Sbjct: 503 NNNDNDNDNNNSNNNNNNNNNNNNNNNNN 531
Score = 21.2 bits (43), Expect = 0.44
Identities = 9/29 (31%), Positives = 19/29 (65%)
Query: 889 NNDHMIQNNDHMVDSNNNYYISHNNVDKN 917
+ND+ N+++ ++NNN ++NN + N
Sbjct: 506 DNDNDNNNSNNNNNNNNNNNNNNNNNNNN 534
Score = 20.8 bits (42), Expect = 0.58
Identities = 19/92 (20%), Positives = 35/92 (38%), Gaps = 19/92 (20%)
Query: 887 IQNNDHMIQNNDHMVDSNNNYYISHNNVDKNVGDIVXXXXXXXXXXXXIMYNNICKKKES 946
I+ +I NN + D +NN ++N + N NN +
Sbjct: 483 IKEKGGIINNNSNNDDDDNNNNNDNDNDNNNSN------------------NNNNNNNNN 524
Query: 947 GIGNVQKYENDLHNNSSFKYSKNL-NKYENDD 977
N N+ +NN++ S N+ N +N++
Sbjct: 525 NNNNNNNNNNNNNNNNNNNNSNNIKNNVDNNN 556
Score = 20.8 bits (42), Expect = 0.58
Identities = 8/17 (47%), Positives = 11/17 (64%)
Query: 22 DMNSSYNLNEHKNNKNS 38
D N+S N N + NN N+
Sbjct: 510 DNNNSNNNNNNNNNNNN 526
Score = 20.4 bits (41), Expect = 0.76
Identities = 8/22 (36%), Positives = 14/22 (63%)
Query: 1046 SRHNDNNVESNVLNPYNTNSYN 1067
+ +NDN+ ++N N N N+ N
Sbjct: 502 NNNNDNDNDNNNSNNNNNNNNN 523
Score = 20.4 bits (41), Expect = 0.76
Identities = 9/19 (47%), Positives = 11/19 (57%)
Query: 1049 NDNNVESNVLNPYNTNSYN 1067
NDNN +N N N N+ N
Sbjct: 509 NDNNNSNNNNNNNNNNNNN 527
Score = 20.4 bits (41), Expect = 0.76
Identities = 9/22 (40%), Positives = 13/22 (59%)
Query: 1046 SRHNDNNVESNVLNPYNTNSYN 1067
S +N+NN +N N N N+ N
Sbjct: 514 SNNNNNNNNNNNNNNNNNNNNN 535
Score = 20.0 bits (40), Expect = 0.99
Identities = 9/19 (47%), Positives = 14/19 (73%), Gaps = 1/19 (5%)
Query: 1046 SRHNDNNVESNVLNPYNTN 1064
+ +N NN+++NV N NTN
Sbjct: 541 NNNNSNNIKNNVDN-NNTN 558
Score = 19.2 bits (38), Expect = 1.7
Identities = 7/15 (46%), Positives = 10/15 (66%)
Query: 24 NSSYNLNEHKNNKNS 38
NS+ N N + NN N+
Sbjct: 513 NSNNNNNNNNNNNNN 527
Score = 19.2 bits (38), Expect = 1.7
Identities = 8/22 (36%), Positives = 13/22 (59%)
Query: 1046 SRHNDNNVESNVLNPYNTNSYN 1067
+ +N+NN +N N N N+ N
Sbjct: 515 NNNNNNNNNNNNNNNNNNNNNN 536
Score = 19.2 bits (38), Expect = 1.7
Identities = 8/22 (36%), Positives = 13/22 (59%)
Query: 1046 SRHNDNNVESNVLNPYNTNSYN 1067
+ +N+NN +N N N N+ N
Sbjct: 516 NNNNNNNNNNNNNNNNNNNNNN 537
Score = 19.2 bits (38), Expect = 1.7
Identities = 8/22 (36%), Positives = 13/22 (59%)
Query: 1046 SRHNDNNVESNVLNPYNTNSYN 1067
+ +N+NN +N N N N+ N
Sbjct: 517 NNNNNNNNNNNNNNNNNNNNNN 538
Score = 19.2 bits (38), Expect = 1.7
Identities = 8/22 (36%), Positives = 13/22 (59%)
Query: 1046 SRHNDNNVESNVLNPYNTNSYN 1067
+ +N+NN +N N N N+ N
Sbjct: 518 NNNNNNNNNNNNNNNNNNNNNN 539
Score = 19.2 bits (38), Expect = 1.7
Identities = 8/22 (36%), Positives = 13/22 (59%)
Query: 1046 SRHNDNNVESNVLNPYNTNSYN 1067
+ +N+NN +N N N N+ N
Sbjct: 519 NNNNNNNNNNNNNNNNNNNNNN 540
Score = 19.2 bits (38), Expect = 1.7
Identities = 8/22 (36%), Positives = 13/22 (59%)
Query: 1046 SRHNDNNVESNVLNPYNTNSYN 1067
+ +N+NN +N N N N+ N
Sbjct: 520 NNNNNNNNNNNNNNNNNNNNNN 541
Score = 19.2 bits (38), Expect = 1.7
Identities = 8/22 (36%), Positives = 13/22 (59%)
Query: 1046 SRHNDNNVESNVLNPYNTNSYN 1067
+ +N+NN +N N N N+ N
Sbjct: 521 NNNNNNNNNNNNNNNNNNNNNN 542
Score = 19.2 bits (38), Expect = 1.7
Identities = 8/22 (36%), Positives = 13/22 (59%)
Query: 1046 SRHNDNNVESNVLNPYNTNSYN 1067
+ +N+NN +N N N N+ N
Sbjct: 522 NNNNNNNNNNNNNNNNNNNNNN 543
Score = 19.2 bits (38), Expect = 1.7
Identities = 8/22 (36%), Positives = 13/22 (59%)
Query: 1046 SRHNDNNVESNVLNPYNTNSYN 1067
+ +N+NN +N N N N+ N
Sbjct: 523 NNNNNNNNNNNNNNNNNNNNNN 544
Score = 19.2 bits (38), Expect = 1.7
Identities = 8/22 (36%), Positives = 13/22 (59%)
Query: 1046 SRHNDNNVESNVLNPYNTNSYN 1067
+ +N+NN +N N N N+ N
Sbjct: 525 NNNNNNNNNNNNNNNNNNNNSN 546
Score = 18.9 bits (37), Expect = 2.2
Identities = 11/43 (25%), Positives = 16/43 (37%)
Query: 120 SCNNKYNKNGFVYDYNTYYTKKSSDLKGKLKNENESMNSDGNN 162
S NN N N + N +++ + N N D NN
Sbjct: 514 SNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNSNNIKNNVDNNN 556
Score = 18.9 bits (37), Expect = 2.2
Identities = 11/37 (29%), Positives = 17/37 (45%)
Query: 765 SMVHDKNTEQNIMLNDELNNNKSPNILNKSYIDNNKN 801
SMV NI+ ND+ N+ + L S ++N
Sbjct: 884 SMVKSSFFSSNIVTNDDENSIEEDKNLRYSDASASEN 920
Score = 18.5 bits (36), Expect = 2.9
Identities = 8/20 (40%), Positives = 11/20 (55%)
Query: 1048 HNDNNVESNVLNPYNTNSYN 1067
+N+NN N N N N+ N
Sbjct: 501 NNNNNDNDNDNNNSNNNNNN 520
Score = 18.5 bits (36), Expect = 2.9
Identities = 8/20 (40%), Positives = 11/20 (55%)
Query: 1048 HNDNNVESNVLNPYNTNSYN 1067
+N NN +N N N N+ N
Sbjct: 512 NNSNNNNNNNNNNNNNNNNN 531
Score = 18.1 bits (35), Expect = 3.8
Identities = 7/15 (46%), Positives = 10/15 (66%)
Query: 1046 SRHNDNNVESNVLNP 1060
S + NNV++N NP
Sbjct: 545 SNNIKNNVDNNNTNP 559
Score = 17.7 bits (34), Expect = 4.9
Identities = 8/32 (25%), Positives = 18/32 (56%)
Query: 886 MIQNNDHMIQNNDHMVDSNNNYYISHNNVDKN 917
+I NN + ++++ + N+N + NN + N
Sbjct: 489 IINNNSNNDDDDNNNNNDNDNDNNNSNNNNNN 520
Score = 17.3 bits (33), Expect = 6.4
Identities = 7/20 (35%), Positives = 12/20 (60%)
Query: 1048 HNDNNVESNVLNPYNTNSYN 1067
+++NN +N N N N+ N
Sbjct: 509 NDNNNSNNNNNNNNNNNNNN 528
Score = 17.3 bits (33), Expect = 6.4
Identities = 7/22 (31%), Positives = 13/22 (59%)
Query: 1046 SRHNDNNVESNVLNPYNTNSYN 1067
+ +++NN +N N N N+ N
Sbjct: 511 NNNSNNNNNNNNNNNNNNNNNN 532
Score = 17.3 bits (33), Expect = 6.4
Identities = 8/29 (27%), Positives = 11/29 (37%)
Query: 889 NNDHMIQNNDHMVDSNNNYYISHNNVDKN 917
NNDH + N + H +V N
Sbjct: 855 NNDHFSLPGLKFALNQNKSHDKHQDVSTN 883
Lambda K H
0.311 0.129 0.358
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 3626
Number of extensions: 416
Number of successful extensions: 202
Number of sequences better than 10.0: 1
Number of HSP's gapped: 72
Number of HSP's successfully gapped: 60
Length of query: 1190
Length of database: 961
Length adjustment: 45
Effective length of query: 1145
Effective length of database: 916
Effective search space: 1048820
Effective search space used: 1048820
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 32 (17.3 bits)
S2: 32 (16.9 bits)