>YDL058W USO1 SGDID:S000002216, Chr IV from 345665-351037, Verified
            ORF, "Essential protein involved in intracellular protein
            transport, coiled-coil protein necessary for transport
            from ER to Golgi; required for assembly of the
            ER-to-Golgi SNARE complex"
          Length = 1790

 Score = 32.3 bits (72), Expect = 2e-04
 Identities = 25/118 (21%), Positives = 56/118 (47%), Gaps = 16/118 (13%)

Query: 344  KNKFEDVIKLCNIQQF---TNQDDSQLLEISKKDKCKEALKHYEEWVNRRRPEWKGQCDK 400
            K+K ED+ +    +Q    +NQ++ +LL  S+  + ++ L   ++   +   E + +  K
Sbjct: 1609 KSKLEDIERELKDKQAEIKSNQEEKELLT-SRLKELEQELDSTQQKAQKSEEERRAEVRK 1667

Query: 401  FEKEKSKYEDTKSITAEKYLKEICSECDCKYKDLDNTFKEFKDNVTLLKAVIDNKKNQ 458
            F+ EKS+ ++   +             + KY DL N  + +K +   +K   D+++ +
Sbjct: 1668 FQVEKSQLDEKAML------------LETKYNDLVNKEQAWKRDEDTVKKTTDSQRQE 1713

 Score = 30.8 bits (68), Expect = 6e-04
 Identities = 41/177 (23%), Positives = 69/177 (38%), Gaps = 26/177 (14%)

Query: 334  EWGTYVCSEYKNKFEDVIKLCNIQQFTNQDDSQLLEIS----KKDKCKEALKHYEEW--- 386
            E G       K K ED I       F    + Q +E +    +K+K K  + H +E    
Sbjct: 845  EKGLETILSQKKKAEDGINKMGKDLFALSREMQAVEENCKNLQKEKDKSNVNHQKETKSL 904

Query: 387  -------------VNRRRPEWKGQCDKFEKEKSKYEDTKSITAEKYLK--EICSECDCKY 431
                         +N    E K QC+   KEK            ++     + ++   K 
Sbjct: 905  KEDIAAKITEIKAINENLEEMKIQCNNLSKEKEHISKELVEYKSRFQSHDNLVAKLTEKL 964

Query: 432  KDLDNTFKEFK-DNVTLLKAVIDNKKNQDXXXXXXXXXXINSV-RDSSNLD-QRGNI 485
            K L N +K+ + +N +L+KAV +  KN+           I+S+ ++  N   +RG+I
Sbjct: 965  KSLANNYKDMQAENESLIKAV-EESKNESSIQLSNLQNKIDSMSQEKENFQIERGSI 1020

 Score = 27.7 bits (60), Expect = 0.005
 Identities = 58/275 (21%), Positives = 114/275 (41%), Gaps = 44/275 (16%)

Query: 198  RNINKVWHRIKDEKNFKEEFVK---VALGESNALMKHYKEKNLNALTAIKYGFSDMGDII 254
            +NI ++   I D +  KEE +     +  E  + +   KEK   A TA     + + ++ 
Sbjct: 1022 KNIEQLKKTISDLEQTKEEIISKSDSSKDEYESQISLLKEKLETATTANDENVNKISELT 1081

Query: 255  KGTDLIDYQIT--KNINRALDKILRNETSNDKIKKRVDWWEANKSAFWDAFMCGYKVHIG 312
            K  + ++ ++   KN+   L+  L  ETS   +K+  +  E  K           K+ + 
Sbjct: 1082 KTREELEAELAAYKNLKNELETKL--ETSEKALKEVKENEEHLKEE---------KIQLE 1130

Query: 313  NKPCPEHDNMDRIPQYLRWFREWGTYVCSEYKNKFEDVIKLCNIQQFTNQDDSQLL-EIS 371
             +       ++ +   L    +    + ++ K K+E+  ++ N ++  N++ SQL  EI+
Sbjct: 1131 KEATETKQQLNSLRANLESLEKEHEDLAAQLK-KYEE--QIANKERQYNEEISQLNDEIT 1187

Query: 372  KKDKCKEALKH---------------YEEWVNRRRPEWKG---QCDKFEK-----EKSKY 408
               +  E++K                 EE  N ++ E      Q  + +K     E S  
Sbjct: 1188 STQQENESIKKKNDELEGEVKAMKSTSEEQSNLKKSEIDALNLQIKELKKKNETNEASLL 1247

Query: 409  EDTKSITAEKY-LKEICSECDCKYKDLDNTFKEFK 442
            E  KS+ +E   +KE+  EC+ K K++     + K
Sbjct: 1248 ESIKSVESETVKIKELQDECNFKEKEVSELEDKLK 1282

 Score = 26.9 bits (58), Expect = 0.009
 Identities = 17/71 (23%), Positives = 29/71 (40%)

Query: 627  LSNQQQSEKKSISKVDEDSYRILSVSYKDNNEVKNVAESIVKKLFSLFNDNNNLETIFKG 686
            L  +   EK  + K  E   ++ S    +  E+K+  E+I K    L     + E   K 
Sbjct: 1489 LEEESSKEKAELEKSKEMMKKLESTIESNETELKSSMETIRKSDEKLEQSKKSAEEDIKN 1548

Query: 687  LTEDMTDLFQK 697
            L  + +DL  +
Sbjct: 1549 LQHEKSDLISR 1559

 Score = 26.6 bits (57), Expect = 0.011
 Identities = 15/67 (22%), Positives = 33/67 (49%), Gaps = 6/67 (8%)

Query: 397 QCDKFEKEKSKYEDTKSITAEKYLKEICS------ECDCKYKDLDNTFKEFKDNVTLLKA 450
           QC K + E +  +     T E   +++ +      E D KY+ L+++    K+N ++L+ 
Sbjct: 738 QCTKLKGEITSLQTETESTHENLTEKLIALTNEHKELDEKYQILNSSHSSLKENFSILET 797

Query: 451 VIDNKKN 457
            + N ++
Sbjct: 798 ELKNVRD 804

 Score = 26.2 bits (56), Expect = 0.015
 Identities = 58/310 (18%), Positives = 115/310 (37%), Gaps = 37/310 (11%)

Query: 263  QITKNINRALDKILRNETSNDKIKKRVDWWEANKSAFWDAFMCGYKVHIGNKPCPEHDNM 322
            Q  + I++  D+I   +  N+ IKK+ D  E    A     M        N    E D +
Sbjct: 1174 QYNEEISQLNDEITSTQQENESIKKKNDELEGEVKA-----MKSTSEEQSNLKKSEIDAL 1228

Query: 323  DRIPQYLRWFREWG-TYVCSEYKNKFEDVIKLCNIQQFTNQDDSQLLEISKKDKCKEALK 381
            +   + L+   E     +    K+   + +K+  +Q   N  + ++ E+  K K  E   
Sbjct: 1229 NLQIKELKKKNETNEASLLESIKSVESETVKIKELQDECNFKEKEVSELEDKLKASED-- 1286

Query: 382  HYEEWVNRRRPEWKGQCDKFEKEKSKYEDTKSITAEKY--LKEICSECDCKYKDLDNTFK 439
                  N +  E + + +K ++E         I  EK   L +   + + +   L  T  
Sbjct: 1287 -----KNSKYLELQKESEKIKEELDAKTTELKIQLEKITNLSKAKEKSESELSRLKKTSS 1341

Query: 440  EFKDNVTLLKAVIDNK---KNQDXXXXXXXXXXINSVRDSSNLDQRGNITTSQGNSHRAT 496
            E + N       + N+   KNQ             +      L   G+ T +Q  S +  
Sbjct: 1342 EERKNAEEQLEKLKNEIQIKNQ-------------AFEKERKLLNEGSSTITQEYSEKIN 1388

Query: 497  VVQQVDQTNRLDNVNSVTQRGXXXXXXXLERGLGSGALPGTNIITEEKYSLELIKLTSKD 556
             ++  D+  RL N N +  +        LE+     +L    ++ E++ +++ ++     
Sbjct: 1389 TLE--DELIRLQNENELKAKEIDNTRSELEK----VSLSNDELLEEKQNTIKSLQDEILS 1442

Query: 557  EEDIIKHNED 566
             +D I  N++
Sbjct: 1443 YKDKITRNDE 1452

 Score = 23.5 bits (49), Expect = 0.097
 Identities = 27/99 (27%), Positives = 50/99 (50%), Gaps = 14/99 (14%)

Query: 373  KDKCKEALKHYEEWVNR------RRPEWKGQCDKFEKEKSKYEDTKSITAEKYLKEICSE 426
            K+K + A    +E VN+       R E + +   ++  K++ E TK  T+EK LKE+  E
Sbjct: 1060 KEKLETATTANDENVNKISELTKTREELEAELAAYKNLKNELE-TKLETSEKALKEV-KE 1117

Query: 427  CDCKYKD----LDNTFKEFKDNVTLLKAVIDN--KKNQD 459
             +   K+    L+    E K  +  L+A +++  K+++D
Sbjct: 1118 NEEHLKEEKIQLEKEATETKQQLNSLRANLESLEKEHED 1156

 Score = 22.3 bits (46), Expect = 0.22
 Identities = 22/83 (26%), Positives = 40/83 (48%), Gaps = 9/83 (10%)

Query: 207  IKDEKNFKEEFVKVALGESNALMKHYKEKNLNALTAIKYGFSDMGDIIKGTDLIDYQITK 266
            ++DE NFKE+ V     E    +K  ++KN   L   K       ++   T  +  Q+ K
Sbjct: 1263 LQDECNFKEKEV----SELEDKLKASEDKNSKYLELQKESEKIKEELDAKTTELKIQLEK 1318

Query: 267  --NINRALDKILRNETSNDKIKK 287
              N+++A +K   +E+   ++KK
Sbjct: 1319 ITNLSKAKEK---SESELSRLKK 1338

 Score = 19.6 bits (39), Expect = 1.4
 Identities = 16/79 (20%), Positives = 30/79 (37%), Gaps = 1/79 (1%)

Query: 7   IFFSLFILNLHIYIKNIKCNDLINYXXXXXXXXXXXXXXXXTNGLNNKDNSFIDSKIEEH 66
           I + L+  ++H +   + C+ L+                      NN  N+  D+  E  
Sbjct: 404 INWMLYANSVHTFDTRVACSRLLKAYFMDNFDLQRDFLLKQVQLCNNSTNNVGDNAKENG 463

Query: 67  -ENKSYQNKDNNISIVGQD 84
             NKS +  D++    G+D
Sbjct: 464 GSNKSDKESDSDKDTDGKD 482

 Score = 19.6 bits (39), Expect = 1.4
 Identities = 12/34 (35%), Positives = 16/34 (47%), Gaps = 10/34 (29%)

Query: 87   ITSVYSSKII----------NANDLEGNSIDDTK 110
            IT  YS KI           N N+L+   ID+T+
Sbjct: 1379 ITQEYSEKINTLEDELIRLQNENELKAKEIDNTR 1412

 Score = 16.9 bits (32), Expect = 9.1
 Identities = 6/21 (28%), Positives = 13/21 (61%)

Query: 57   SFIDSKIEEHENKSYQNKDNN 77
            +F + ++ E E+K   ++D N
Sbjct: 1268 NFKEKEVSELEDKLKASEDKN 1288

Lambda     K      H
   0.313    0.132    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 2896
Number of extensions: 155
Number of successful extensions: 15
Number of sequences better than 10.0: 1
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 11
Length of query: 697
Length of database: 1790
Length adjustment: 46
Effective length of query: 651
Effective length of database: 1744
Effective search space:  1135344
Effective search space used:  1135344
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 32 (17.4 bits)
S2: 32 (16.9 bits)