>YDL058W USO1 SGDID:S000002216, Chr IV from 345665-351037, Verified
            ORF, "Essential protein involved in intracellular protein
            transport, coiled-coil protein necessary for transport
            from ER to Golgi; required for assembly of the
            ER-to-Golgi SNARE complex"
          Length = 1790

 Score = 39.3 bits (90), Expect = 1e-06
 Identities = 24/80 (30%), Positives = 44/80 (55%), Gaps = 6/80 (7%)

Query: 122  FKNYEGEILKLETMLKEKEHEYMDLRINFQELLEEKCKLENDMDNYEKAIYEINENLISL 181
            +KN + E   LET L+  E    +++ N + L EEK +LE +    ++ +  +  NL SL
Sbjct: 1094 YKNLKNE---LETKLETSEKALKEVKENEEHLKEEKIQLEKEATETKQQLNSLRANLESL 1150

Query: 182  KEAHKE---ELKEVHEENTN 198
            ++ H++   +LK+  E+  N
Sbjct: 1151 EKEHEDLAAQLKKYEEQIAN 1170

 Score = 33.9 bits (76), Expect = 5e-05
 Identities = 25/101 (24%), Positives = 47/101 (46%), Gaps = 3/101 (2%)

Query: 117 FHVYIFKNYEGEILKLETMLKEKEHEYMDLRINFQEL--LEEKC-KLENDMDNYEKAIYE 173
           F  Y  + +   I ++ T L     E    +I+F+E+  L+ +C KL+ ++ + +     
Sbjct: 696 FSTYFIQLFNENIYRIRTALSHDPDEEPINKISFEEVEKLQRQCTKLKGEITSLQTETES 755

Query: 174 INENLISLKEAHKEELKEVHEENTNLYSTIDKFKDKINTIE 214
            +ENL     A   E KE+ E+   L S+    K+  + +E
Sbjct: 756 THENLTEKLIALTNEHKELDEKYQILNSSHSSLKENFSILE 796

 Score = 32.3 bits (72), Expect = 1e-04
 Identities = 26/120 (21%), Positives = 57/120 (47%), Gaps = 7/120 (5%)

Query: 131  KLETMLKEKEHEYMDLRINFQELLEEKCKLENDMDNYE-------KAIYEINENLISLKE 183
            K+E  LK+ E E    +   ++  E   KLE+ +++ E       + I + +E L   K+
Sbjct: 1481 KVEEGLKKLEEESSKEKAELEKSKEMMKKLESTIESNETELKSSMETIRKSDEKLEQSKK 1540

Query: 184  AHKEELKEVHEENTNLYSTIDKFKDKINTIENLXXXXXXXXXXXXXVGEKLDLVQSQQRM 243
            + +E++K +  E ++L S I++ +  I  +++              V ++L+  Q + R+
Sbjct: 1541 SAEEDIKNLQHEKSDLISRINESEKDIEELKSKLRIEAKSGSELETVKQELNNAQEKIRI 1600

 Score = 30.8 bits (68), Expect = 4e-04
 Identities = 23/92 (25%), Positives = 42/92 (45%), Gaps = 4/92 (4%)

Query: 123 KNYEGEILKLETMLKEKEHEYMDLRINFQELLEEKCKLENDMDNYEKAIYEINENLISLK 182
           +N   +++ L    KE + +Y  L  +   L E    LE ++ N   ++ E+ +    L+
Sbjct: 758 ENLTEKLIALTNEHKELDEKYQILNSSHSSLKENFSILETELKNVRDSLDEMTQ----LR 813

Query: 183 EAHKEELKEVHEENTNLYSTIDKFKDKINTIE 214
           +  + + KE         STI K +D I T+E
Sbjct: 814 DVLETKDKENQTALLEYKSTIHKQEDSIKTLE 845

 Score = 28.9 bits (63), Expect = 0.002
 Identities = 16/58 (27%), Positives = 31/58 (53%), Gaps = 4/58 (6%)

Query: 157  KCKLENDMDNYEKAIYEINENLISLKEAHKEELKEVHEENTNLYSTIDKFKDKINTIE 214
            K +LE  ++  EKA+ E+ EN    +E  KEE  ++ +E T     ++  +  + ++E
Sbjct: 1098 KNELETKLETSEKALKEVKEN----EEHLKEEKIQLEKEATETKQQLNSLRANLESLE 1151

 Score = 27.7 bits (60), Expect = 0.003
 Identities = 20/87 (22%), Positives = 47/87 (54%), Gaps = 8/87 (9%)

Query: 131  KLETMLKEKEHEYMDLRINFQELLEEKCKLENDMDNYEKAIYEIN-------ENLISLKE 183
            K+++M +EKE+  ++ R + ++ +E+  K  +D++  ++ I   +       E+ ISL +
Sbjct: 1002 KIDSMSQEKENFQIE-RGSIEKNIEQLKKTISDLEQTKEEIISKSDSSKDEYESQISLLK 1060

Query: 184  AHKEELKEVHEENTNLYSTIDKFKDKI 210
               E     ++EN N  S + K ++++
Sbjct: 1061 EKLETATTANDENVNKISELTKTREEL 1087

 Score = 27.3 bits (59), Expect = 0.004
 Identities = 23/95 (24%), Positives = 45/95 (47%), Gaps = 11/95 (11%)

Query: 123  KNYEGEILKLETMLKE---KEHEYMDLRINFQELLEEKCKLENDMDNYEKAIYEINENLI 179
            K+ E E +K++ +  E   KE E  +L        E+K K   D ++    + + +E + 
Sbjct: 1251 KSVESETVKIKELQDECNFKEKEVSEL--------EDKLKASEDKNSKYLELQKESEKIK 1302

Query: 180  SLKEAHKEELKEVHEENTNLYSTIDKFKDKINTIE 214
               +A   ELK   E+ TNL    +K + +++ ++
Sbjct: 1303 EELDAKTTELKIQLEKITNLSKAKEKSESELSRLK 1337

 Score = 26.6 bits (57), Expect = 0.008
 Identities = 21/65 (32%), Positives = 31/65 (47%), Gaps = 1/65 (1%)

Query: 131  KLETMLKEKEHEYMDLRINFQELLEEKCKLENDMDNYEKAIYEINENLISLKEAHKEELK 190
            +LET+ +E  +    +RIN +E    K KLE+     +    EI  N    KE     LK
Sbjct: 1583 ELETVKQELNNAQEKIRINAEENTVLKSKLEDIERELKDKQAEIKSNQ-EEKELLTSRLK 1641

Query: 191  EVHEE 195
            E+ +E
Sbjct: 1642 ELEQE 1646

 Score = 26.2 bits (56), Expect = 0.010
 Identities = 14/44 (31%), Positives = 25/44 (56%)

Query: 383  NFQNKIDILDNKMQEFKNEITNEKKLNYQLKILYNKLKEKENDI 426
            N QNKID +  + + F+ E  + +K   QLK   + L++ + +I
Sbjct: 998  NLQNKIDSMSQEKENFQIERGSIEKNIEQLKKTISDLEQTKEEI 1041

 Score = 25.8 bits (55), Expect = 0.013
 Identities = 12/32 (37%), Positives = 19/32 (59%)

Query: 7    ELQNKNSISTELLQEIKKMENELKRKNDEYEK 38
            EL+ K   S + L+E+K+ E  LK +  + EK
Sbjct: 1100 ELETKLETSEKALKEVKENEEHLKEEKIQLEK 1131

 Score = 25.4 bits (54), Expect = 0.017
 Identities = 28/98 (28%), Positives = 42/98 (42%), Gaps = 15/98 (15%)

Query: 121  IFKNYEGEILKLETMLKEKEH---EYMDLRINFQE------LLEEKCKLENDMDNYEKAI 171
            I +N E   ++   + KEKEH   E ++ +  FQ        L EK K    + N  K +
Sbjct: 918  INENLEEMKIQCNNLSKEKEHISKELVEYKSRFQSHDNLVAKLTEKLK---SLANNYKDM 974

Query: 172  YEINENLISLKEAHKEELKEVHEENTNLYSTIDKFKDK 209
               NE+LI   E  K E      + +NL + ID    +
Sbjct: 975  QAENESLIKAVEESKNESS---IQLSNLQNKIDSMSQE 1009

 Score = 24.3 bits (51), Expect = 0.038
 Identities = 47/226 (20%), Positives = 82/226 (36%), Gaps = 57/226 (25%)

Query: 24   KMENE-LKRKNDEYEKLNANYREIYGSYILAKTNLDDTTNVRXXXXXXXXXNVFLLFMST 82
            + ENE +K+KNDE E          G     K+  ++ +N++          +  L    
Sbjct: 1190 QQENESIKKKNDELE----------GEVKAMKSTSEEQSNLKKSEIDALNLQIKELKKKN 1239

Query: 83   YFEYSTILRLFHILXXXXXXXXXXXXXXXXXXXXFHVYIFKNYEGEILKLETMLK---EK 139
                +++L     +                         FK  E E+ +LE  LK   +K
Sbjct: 1240 ETNEASLLESIKSVESETVKIKELQDECN----------FK--EKEVSELEDKLKASEDK 1287

Query: 140  EHEYMDLRINFQELLEE--------KCKLENDMDNYEKAIYEINENLISLKEAHKEELKE 191
              +Y++L+   +++ EE        K +LE  + N  KA  +    L  LK+   EE K 
Sbjct: 1288 NSKYLELQKESEKIKEELDAKTTELKIQLEK-ITNLSKAKEKSESELSRLKKTSSEERKN 1346

Query: 192  VHEENTNLYSTI----------------------DKFKDKINTIEN 215
              E+   L + I                       ++ +KINT+E+
Sbjct: 1347 AEEQLEKLKNEIQIKNQAFEKERKLLNEGSSTITQEYSEKINTLED 1392

 Score = 24.3 bits (51), Expect = 0.038
 Identities = 13/40 (32%), Positives = 23/40 (57%), Gaps = 4/40 (10%)

Query: 3    KSICELQNKNSISTE----LLQEIKKMENELKRKNDEYEK 38
            KS  EL      S+E      ++++K++NE++ KN  +EK
Sbjct: 1328 KSESELSRLKKTSSEERKNAEEQLEKLKNEIQIKNQAFEK 1367

 Score = 23.1 bits (48), Expect = 0.085
 Identities = 24/89 (26%), Positives = 42/89 (47%), Gaps = 4/89 (4%)

Query: 131  KLETMLKEKEHEYMDLRINFQELLEEKCKLENDMDN--YEKAIYEINENLISLKEAHKEE 188
            K E+ L   +    + R N +E LE K K E  + N  +EK    +NE   ++ + + E+
Sbjct: 1328 KSESELSRLKKTSSEERKNAEEQLE-KLKNEIQIKNQAFEKERKLLNEGSSTITQEYSEK 1386

Query: 189  LKEVHEENTNLYSTID-KFKDKINTIENL 216
            +  + +E   L +  + K K+  NT   L
Sbjct: 1387 INTLEDELIRLQNENELKAKEIDNTRSEL 1415

 Score = 21.6 bits (44), Expect = 0.25
 Identities = 8/30 (26%), Positives = 18/30 (60%)

Query: 17   ELLQEIKKMENELKRKNDEYEKLNANYREI 46
            ++ +E+K  + E+K   +E E L +  +E+
Sbjct: 1614 DIERELKDKQAEIKSNQEEKELLTSRLKEL 1643

 Score = 20.8 bits (42), Expect = 0.42
 Identities = 23/114 (20%), Positives = 51/114 (44%), Gaps = 15/114 (13%)

Query: 126  EGEILKLETMLKE----KEHEYMDLRINFQELLEEKCKLENDMDNYEKAIYEINENLISL 181
            EGE+  +++  +E    K+ E   L +  +EL     K +N+ +  E ++ E  +++   
Sbjct: 1204 EGEVKAMKSTSEEQSNLKKSEIDALNLQIKEL-----KKKNETN--EASLLESIKSV--- 1253

Query: 182  KEAHKEELKEVHEENTNLYSTIDKFKDKINTIENLXXXXXXXXXXXXXVGEKLD 235
             E+   ++KE+ +E       + + +DK+   E+              + E+LD
Sbjct: 1254 -ESETVKIKELQDECNFKEKEVSELEDKLKASEDKNSKYLELQKESEKIKEELD 1306

 Score = 20.4 bits (41), Expect = 0.55
 Identities = 14/48 (29%), Positives = 22/48 (45%), Gaps = 2/48 (4%)

Query: 152 ELLEEKCKLENDMDNYEKAIYEINENLISLKEAHKEELKEVHEENTNL 199
           + +EE CK  N     +K+     +   SLKE    ++ E+   N NL
Sbjct: 877 QAVEENCK--NLQKEKDKSNVNHQKETKSLKEDIAAKITEIKAINENL 922

 Score = 20.4 bits (41), Expect = 0.55
 Identities = 13/40 (32%), Positives = 19/40 (47%), Gaps = 6/40 (15%)

Query: 2    TKSICELQNKNSISTELLQEIKKMENELK------RKNDE 35
            +K   EL+    +  +L   I+  E ELK      RK+DE
Sbjct: 1494 SKEKAELEKSKEMMKKLESTIESNETELKSSMETIRKSDE 1533

 Score = 19.6 bits (39), Expect = 0.94
 Identities = 14/48 (29%), Positives = 26/48 (54%), Gaps = 6/48 (12%)

Query: 2    TKSICELQNKNSISTELLQEIKKMENELKR---KNDEYEKLNANYREI 46
            T  I ELQ++ +   +   E+ ++E++LK    KN +Y +L     +I
Sbjct: 1257 TVKIKELQDECNFKEK---EVSELEDKLKASEDKNSKYLELQKESEKI 1301

 Score = 19.2 bits (38), Expect = 1.2
 Identities = 7/31 (22%), Positives = 15/31 (48%)

Query: 5    ICELQNKNSISTELLQEIKKMENELKRKNDE 35
            +  LQN+N +  + +   +    ++   NDE
Sbjct: 1394 LIRLQNENELKAKEIDNTRSELEKVSLSNDE 1424

 Score = 18.9 bits (37), Expect = 1.6
 Identities = 8/31 (25%), Positives = 16/31 (51%)

Query: 15   STELLQEIKKMENELKRKNDEYEKLNANYRE 45
            +TE  Q++  +   L+    E+E L A  ++
Sbjct: 1133 ATETKQQLNSLRANLESLEKEHEDLAAQLKK 1163

 Score = 18.5 bits (36), Expect = 2.1
 Identities = 14/55 (25%), Positives = 25/55 (45%), Gaps = 6/55 (10%)

Query: 383  NFQNKIDILDNKMQEFKNEITNEKKLNYQLKILYNKLKEKENDITLFQLWFGYVD 437
            + Q + + L   ++E KNE +       QL  L NK+     +   FQ+  G ++
Sbjct: 973  DMQAENESLIKAVEESKNESS------IQLSNLQNKIDSMSQEKENFQIERGSIE 1021

 Score = 18.1 bits (35), Expect = 2.7
 Identities = 8/36 (22%), Positives = 19/36 (52%)

Query: 391  LDNKMQEFKNEITNEKKLNYQLKILYNKLKEKENDI 426
            L+N  ++ +        L  +L+ +  +LK+K+ +I
Sbjct: 1591 LNNAQEKIRINAEENTVLKSKLEDIERELKDKQAEI 1626

 Score = 16.9 bits (32), Expect = 6.1
 Identities = 6/29 (20%), Positives = 15/29 (51%)

Query: 9    QNKNSISTELLQEIKKMENELKRKNDEYE 37
            + ++++    +  +     ELK+KN+  E
Sbjct: 1215 EEQSNLKKSEIDALNLQIKELKKKNETNE 1243

Lambda     K      H
   0.316    0.135    0.373 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 2121
Number of extensions: 139
Number of successful extensions: 28
Number of sequences better than 10.0: 1
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 25
Length of query: 479
Length of database: 1790
Length adjustment: 43
Effective length of query: 436
Effective length of database: 1747
Effective search space:   761692
Effective search space used:   761692
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 31 (17.0 bits)
S2: 31 (16.5 bits)