>YNL227C JJJ1 SGDID:S000005171, Chr XIV from 222432-220660, reverse
           complement, Uncharacterized ORF, "Protein that contains
           a 70 amino acid J-domain, may function as a co-chaperone
           to recruit Hsp70-like activity to specific sites;
           mutation causes defects in fluid-phase endocytosis"
          Length = 590

 Score = 36.6 bits (83), Expect = 9e-06
 Identities = 33/117 (28%), Positives = 52/117 (44%), Gaps = 7/117 (5%)

Query: 52  KYENKTKKVEINEFIGEDENLKVKLYGKEDIQEEDIIKRCLFCDKDNKILEQLSYRILYD 111
           K EN  +  ++ +  GE EN       K D + E +I  C  C+K  K  +QL   I   
Sbjct: 307 KEENWDELEKVYDNFGEFEN------SKNDKEGEVLIYECFICNKTFKSEKQLKNHINTK 360

Query: 112 VMKKCIDHENVLMKIEELGFTADNIKD-EKDENINICSKYKVDTFLDYIYKNLNVIK 167
           + KK ++     M+ E +    DN+ D EK ++ +   K K D  L  +   L  I+
Sbjct: 361 LHKKNMEEIRKEMEEENITLGLDNLSDLEKFDSADESVKEKEDIDLQALQAELAEIE 417

 Score = 26.2 bits (56), Expect = 0.013
 Identities = 35/159 (22%), Positives = 65/159 (40%), Gaps = 29/159 (18%)

Query: 1239 NKYIKKFYFYLNSLTFYLKECKKLDVSYTKPLFYEILPIIMKSLFFINKKVTSKLKKNEL 1298
            NK +K+F  ++  L   +KE  K+                        +K+  + +KNEL
Sbjct: 243  NKTVKRFVVFIKKLDKRMKEGAKIAEE--------------------QRKLKEQQRKNEL 282

Query: 1299 FKHLVDIGRSNIKEFLFHVTPGLVLEEANCKKI-STYILYLLIKNYKIEYEYQVQLFSIC 1357
              +    G  N  E  FH+     ++E N  ++   Y  +   +N K + E +V ++  C
Sbjct: 283  -NNRRKFGNDNNDEEKFHLQSWQTVKEENWDELEKVYDNFGEFENSKNDKEGEVLIYE-C 340

Query: 1358 LILSNA--SEKNLLKVFIKYLLTCIYTFNEDIVLNNMKE 1394
             I +    SEK L      ++ T ++  N + +   M+E
Sbjct: 341  FICNKTFKSEKQLK----NHINTKLHKKNMEEIRKEMEE 375

 Score = 24.6 bits (52), Expect = 0.037
 Identities = 11/28 (39%), Positives = 20/28 (71%), Gaps = 1/28 (3%)

Query: 999  NKKKSNRKQMKSIHVSEKLYCQNMKTLR 1026
            NK   + KQ+K+ H++ KL+ +NM+ +R
Sbjct: 344  NKTFKSEKQLKN-HINTKLHKKNMEEIR 370

 Score = 20.4 bits (41), Expect = 0.69
 Identities = 17/50 (34%), Positives = 26/50 (52%), Gaps = 11/50 (22%)

Query: 66  IGEDENLKVKLYGK-----EDIQEEDI-----IKRC-LFCDKDNKILEQL 104
           + +DE L  K  GK     +D+ E+DI     +K C  F +K +K+L  L
Sbjct: 131 LAKDEILSGKRLGKFSEYQDDVFEQDINSIGYLKACDNFINKTDKLLYPL 180

 Score = 20.4 bits (41), Expect = 0.69
 Identities = 12/52 (23%), Positives = 20/52 (38%)

Query: 452 DNMNELTQNIFINVYNILNCTHLIHKHMILLYFSFNFLKMSSYEYSYFIVVN 503
           DN    T  +   ++      +   KH    + +FN LK  S++  Y    N
Sbjct: 167 DNFINKTDKLLYPLFGYSPTDYEYLKHFYKTWSAFNTLKSFSWKDEYMYSKN 218

 Score = 19.2 bits (38), Expect = 1.5
 Identities = 15/61 (24%), Positives = 28/61 (45%)

Query: 543 KEKGNELNEEFYIFVQKFNNKHKNIYSKLIRRIWSLYMLIISDDYVKNSSNKEDMKINVE 602
           +E  +EL + +  F +  N+K+      LI   +       S+  +KN  N +  K N+E
Sbjct: 308 EENWDELEKVYDNFGEFENSKNDKEGEVLIYECFICNKTFKSEKQLKNHINTKLHKKNME 367

Query: 603 Q 603
           +
Sbjct: 368 E 368

 Score = 18.9 bits (37), Expect = 2.0
 Identities = 7/15 (46%), Positives = 9/15 (60%)

Query: 1673 DSDQEENVQMNTDGK 1687
            D   +ENV +NT  K
Sbjct: 444  DVSSDENVHVNTKNK 458

 Score = 18.1 bits (35), Expect = 3.4
 Identities = 6/17 (35%), Positives = 11/17 (64%)

Query: 1295 KNELFKHLVDIGRSNIK 1311
            +N+LF H+   G + +K
Sbjct: 561  RNKLFNHVKIAGHAAVK 577

 Score = 17.3 bits (33), Expect = 5.9
 Identities = 9/27 (33%), Positives = 15/27 (55%)

Query: 61  EINEFIGEDENLKVKLYGKEDIQEEDI 87
           ++ E   EDE+    L  + DI+ ED+
Sbjct: 419 KLAESSSEDESEDDNLNIEMDIEVEDV 445

 Score = 16.9 bits (32), Expect = 7.7
 Identities = 6/13 (46%), Positives = 9/13 (69%)

Query: 1600 KKNKNTKAIKYNP 1612
            KK    KA++Y+P
Sbjct: 21   KKAYRKKALQYHP 33

 Score = 16.9 bits (32), Expect = 7.7
 Identities = 5/11 (45%), Positives = 8/11 (72%)

Query: 263 SYDENYKIFNY 273
           S+D   K+FN+
Sbjct: 557 SFDSRNKLFNH 567

 Score = 16.9 bits (32), Expect = 7.7
 Identities = 7/24 (29%), Positives = 16/24 (66%)

Query: 1347 YEYQVQLFSICLILSNASEKNLLK 1370
            ++ + +LF+   I  +A+ KN++K
Sbjct: 558  FDSRNKLFNHVKIAGHAAVKNVVK 581

Lambda     K      H
   0.322    0.139    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 2767
Number of extensions: 161
Number of successful extensions: 12
Number of sequences better than 10.0: 1
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 1805
Length of database: 590
Length adjustment: 45
Effective length of query: 1760
Effective length of database: 545
Effective search space:   959200
Effective search space used:   959200
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 32 (17.7 bits)
S2: 32 (16.9 bits)