>YCR072C RSA4 SGDID:S000000668, Chr III from 242348-240801, reverse
           complement, Verified ORF, "WD-repeat protein involved in
           ribosome biogenesis; required for maturation and
           efficient intra-nuclear transport or pre-60S ribosomal
           subunits, localizes to the nucleolus"
          Length = 515

 Score =  178 bits (451), Expect = 6e-49
 Identities = 93/231 (40%), Positives = 144/231 (62%), Gaps = 7/231 (3%)

Query: 322 RSRLASAGKDGSIRINNILSNSVDQILTGHSDTITCILWSGRDTENSTLYSSSRDTTIKI 381
           + RLAS+ KDG+I+I + +S      ++GH+++++C+ W G+      LYS S D T+++
Sbjct: 248 KPRLASSSKDGTIKIWDTVSRVCQYTMSGHTNSVSCVKWGGQ----GLLYSGSHDRTVRV 303

Query: 382 WNVN-EGTLIYNFKGHKHWVNCLTLNSERLLKNGIYNLDXXXXXXXXXXXXEKSKNIMKN 440
           W++N +G  I   K H HWVN L+L+++  L+ G ++              +  +N  K 
Sbjct: 304 WDINSQGRCINILKSHAHWVNHLSLSTDYALRIGAFDHTGKKPSTPEEAQKKALENYEKI 363

Query: 441 FFKNQHHEKI-VSGSDDGTLFLIECLQNKEYKNTRLLGHQKPVIHAQFSPNGKMIVSSSF 499
             KN + E++ V+ SDD T+FL   L++ +    R+ GHQK V H  FSP+G+ IVS+SF
Sbjct: 364 CKKNGNSEEMMVTASDDYTMFLWNPLKSTK-PIARMTGHQKLVNHVAFSPDGRYIVSASF 422

Query: 500 DKSIRVWSAADGKFLAVYRGHVGPVYKVAWSIDNNFFVSCSQDSTLKLWKV 550
           D SI++W   DGKF++ +RGHV  VY+VAWS D    VSCS+D+TLK+W V
Sbjct: 423 DNSIKLWDGRDGKFISTFRGHVASVYQVAWSSDCRLLVSCSKDTTLKVWDV 473

 Score =  142 bits (357), Expect = 5e-38
 Identities = 80/207 (38%), Positives = 128/207 (61%), Gaps = 20/207 (9%)

Query: 30  GPVINVPLSITKDNLDELINNLKRKNEDLGEQEESEDINY-YSFMINDK--------LPI 80
           G  + VP +I++  L+EL+N L   ++D         + Y +S  I  K        + I
Sbjct: 46  GGALRVPGAISEKQLEELLNQLNGTSDD--------PVPYTFSCTIQGKKASDPVKTIDI 97

Query: 81  KNSLYEA-IKDNHISSEDILSIKYFPLNIFKVKKISTCTSTLPGHTSSILCLAFSPN-SS 138
            ++LY + IK  + S+ED +++ Y P  +FKVK ++  +S + GH S+ILC AF+P+ SS
Sbjct: 98  TDNLYSSLIKPGYNSTEDQITLLYTPRAVFKVKPVTRSSSAIAGHGSTILCSAFAPHTSS 157

Query: 139 HLATGSGDNTVRLWDIYTQTPIATLNDHKSWVLVVLFSPDNKFLATAGMDSNVCIYETHT 198
            + TG+GDNT R+WD  TQTP+ TL  H +WVL V +SPD + +AT  MD+ + +++  +
Sbjct: 158 RMVTGAGDNTARIWDCDTQTPMHTLKGHYNWVLCVSWSPDGEVIATGSMDNTIRLWDPKS 217

Query: 199 GKLL-NTLTGHKKEVTTLCFEPLHLLK 224
           G+ L + L GH K +T+L +EP+HL+K
Sbjct: 218 GQCLGDALRGHSKWITSLSWEPIHLVK 244

 Score = 63.2 bits (152), Expect = 3e-14
 Identities = 27/38 (71%), Positives = 31/38 (81%)

Query: 608 LLVDLPGHADAVYAVDWSNDGKFVASGGKDKVLKLWSH 645
           L VDLPGH D VY VDWS DGK V SGGKDK+++LW+H
Sbjct: 478 LSVDLPGHKDEVYTVDWSVDGKRVCSGGKDKMVRLWTH 515

 Score = 61.6 bits (148), Expect = 8e-14
 Identities = 37/133 (27%), Positives = 70/133 (52%), Gaps = 2/133 (1%)

Query: 85  YEAIKDNHISSEDIL--SIKYFPLNIFKVKKISTCTSTLPGHTSSILCLAFSPNSSHLAT 142
           YE I   + +SE+++  +   + + ++   K +   + + GH   +  +AFSP+  ++ +
Sbjct: 360 YEKICKKNGNSEEMMVTASDDYTMFLWNPLKSTKPIARMTGHQKLVNHVAFSPDGRYIVS 419

Query: 143 GSGDNTVRLWDIYTQTPIATLNDHKSWVLVVLFSPDNKFLATAGMDSNVCIYETHTGKLL 202
            S DN+++LWD      I+T   H + V  V +S D + L +   D+ + +++  T KL 
Sbjct: 420 ASFDNSIKLWDGRDGKFISTFRGHVASVYQVAWSSDCRLLVSCSKDTTLKVWDVRTRKLS 479

Query: 203 NTLTGHKKEVTTL 215
             L GHK EV T+
Sbjct: 480 VDLPGHKDEVYTV 492

 Score = 54.3 bits (129), Expect = 1e-11
 Identities = 47/221 (21%), Positives = 89/221 (40%), Gaps = 42/221 (19%)

Query: 317 CSYFV---RSRLASAGKDGSIRINNILSNSVDQILTGHSDTITCILWSGRDTENSTLYSS 373
           CS F     SR+ +   D + RI +  + +    L GH + + C+ WS    +   + + 
Sbjct: 148 CSAFAPHTSSRMVTGAGDNTARIWDCDTQTPMHTLKGHYNWVLCVSWS---PDGEVIATG 204

Query: 374 SRDTTIKIWNVNEGTLIYN-FKGHKHWVNCLTLNSERLLKNGIYNLDXXXXXXXXXXXXE 432
           S D TI++W+   G  + +  +GH  W+  L+     L+K G                  
Sbjct: 205 SMDNTIRLWDPKSGQCLGDALRGHSKWITSLSWEPIHLVKPG------------------ 246

Query: 433 KSKNIMKNFFKNQHHEKIVSGSDDGTLFLIECLQNKEYKNTRLLGHQKPVIHAQFSPNGK 492
                           ++ S S DGT+ + + +         + GH   V   ++   G 
Sbjct: 247 -------------SKPRLASSSKDGTIKIWDTV--SRVCQYTMSGHTNSVSCVKWGGQG- 290

Query: 493 MIVSSSFDKSIRVWSA-ADGKFLAVYRGHVGPVYKVAWSID 532
           ++ S S D+++RVW   + G+ + + + H   V  ++ S D
Sbjct: 291 LLYSGSHDRTVRVWDINSQGRCINILKSHAHWVNHLSLSTD 331

 Score = 54.3 bits (129), Expect = 1e-11
 Identities = 63/285 (22%), Positives = 107/285 (37%), Gaps = 31/285 (10%)

Query: 121 LPGHTSSILCLAFSP-------NSSHLATGSGDNTVRLWDIYTQTPIATLNDHKSWVLVV 173
           L GH+  I  L++ P       +   LA+ S D T+++WD  ++    T++ H + V  V
Sbjct: 225 LRGHSKWITSLSWEPIHLVKPGSKPRLASSSKDGTIKIWDTVSRVCQYTMSGHTNSVSCV 284

Query: 174 LFSPDNKFLATAGMDSNVCIYETHT-GKLLNTLTGHKKEVTTLCFEPLHLLKEEDIENNV 232
            +      L +   D  V +++ ++ G+ +N L  H   V  L     + L+        
Sbjct: 285 KWGGQG-LLYSGSHDRTVRVWDINSQGRCINILKSHAHWVNHLSLSTDYALR----IGAF 339

Query: 233 DHVNKRKINSNEEIDQSDNANKKIKKNDENDVIINGKDNYPSGQHKDKMMPTEGETTXXX 292
           DH  K+     E       A KK  +N E     NG        + ++MM T  +     
Sbjct: 340 DHTGKKPSTPEE-------AQKKALENYEKICKKNG--------NSEEMMVTASDDYTMF 384

Query: 293 XXXXXXXXXXXXXXXXXXXXXXXXCSYFVRSRLASAGKDGSIRINNILSNSVDQILTGHS 352
                                           + SA  D SI++ +           GH 
Sbjct: 385 LWNPLKSTKPIARMTGHQKLVNHVAFSPDGRYIVSASFDNSIKLWDGRDGKFISTFRGHV 444

Query: 353 DTITCILWSGRDTENSTLYSSSRDTTIKIWNVNEGTLIYNFKGHK 397
            ++  + WS   ++   L S S+DTT+K+W+V    L  +  GHK
Sbjct: 445 ASVYQVAWS---SDCRLLVSCSKDTTLKVWDVRTRKLSVDLPGHK 486

 Score = 52.0 bits (123), Expect = 7e-11
 Identities = 45/211 (21%), Positives = 80/211 (37%), Gaps = 49/211 (23%)

Query: 348 LTGHSDTITCILWSGRDTENSTLYSSSRDTTIKIWNVNEGTLIYNFKGHKHWVNCLTLNS 407
           + GH  TI C  ++   +  S + + + D T +IW+ +  T ++  KGH +WV C++ + 
Sbjct: 139 IAGHGSTILCSAFAPHTS--SRMVTGAGDNTARIWDCDTQTPMHTLKGHYNWVLCVSWSP 196

Query: 408 ERLLKNGIYNLDXXXXXXXXXXXXEKSKNIMKNFFKNQHHEKIVSGSDDGTLFLIECLQN 467
           +                                       E I +GS D T+ L +  ++
Sbjct: 197 D--------------------------------------GEVIATGSMDNTIRLWD-PKS 217

Query: 468 KEYKNTRLLGHQKPVIHAQFSP-------NGKMIVSSSFDKSIRVWSAADGKFLAVYRGH 520
            +     L GH K +    + P       +   + SSS D +I++W            GH
Sbjct: 218 GQCLGDALRGHSKWITSLSWEPIHLVKPGSKPRLASSSKDGTIKIWDTVSRVCQYTMSGH 277

Query: 521 VGPVYKVAWSIDNNFFVSCSQDSTLKLWKVS 551
              V  V W        S S D T+++W ++
Sbjct: 278 TNSVSCVKWG-GQGLLYSGSHDRTVRVWDIN 307

 Score = 46.2 bits (108), Expect = 4e-09
 Identities = 59/278 (21%), Positives = 99/278 (35%), Gaps = 46/278 (16%)

Query: 117 CTSTLPGHTSSILCLAFSPNSSHLATGSGDNTVRLWDIYTQ-TPIATLNDHKSWVLVVLF 175
           C  T+ GHT+S+ C+ +      L +GS D TVR+WDI +Q   I  L  H  WV  +  
Sbjct: 270 CQYTMSGHTNSVSCVKWG-GQGLLYSGSHDRTVRVWDINSQGRCINILKSHAHWVNHLSL 328

Query: 176 SPDNKFLATAGMDSNVCIYETHTGKLLNTLTGHKKEVTTLCFEPLHLLKEEDIENNVDHV 235
           S D   L     D        HTGK  +T    +K+                   N + +
Sbjct: 329 STDYA-LRIGAFD--------HTGKKPSTPEEAQKKAL----------------ENYEKI 363

Query: 236 NKRKINSNE-EIDQSDN----------ANKKIKKNDENDVIINGKDNYPSGQHKDKMMPT 284
            K+  NS E  +  SD+          + K I +   +  ++N     P G++       
Sbjct: 364 CKKNGNSEEMMVTASDDYTMFLWNPLKSTKPIARMTGHQKLVNHVAFSPDGRYIVSASFD 423

Query: 285 EGETTXXXXXXXXXXXXXXXXXXXXXXXXXXXCSYFVRSRLASAGKDGSIRINNILSNSV 344
                                           C       L S  KD ++++ ++ +  +
Sbjct: 424 NSIKLWDGRDGKFISTFRGHVASVYQVAWSSDCRL-----LVSCSKDTTLKVWDVRTRKL 478

Query: 345 DQILTGHSDTITCILWSGRDTENSTLYSSSRDTTIKIW 382
              L GH D +  + WS    +   + S  +D  +++W
Sbjct: 479 SVDLPGHKDEVYTVDWS---VDGKRVCSGGKDKMVRLW 513

 Score = 46.2 bits (108), Expect = 4e-09
 Identities = 27/79 (34%), Positives = 43/79 (54%), Gaps = 1/79 (1%)

Query: 119 STLPGHTSSILCLAFSPNSSHLATGSGDNTVRLWDIYTQTPIATLNDHKSWVLVVLFSPD 178
           ST  GH +S+  +A+S +   L + S D T+++WD+ T+     L  HK  V  V +S D
Sbjct: 438 STFRGHVASVYQVAWSSDCRLLVSCSKDTTLKVWDVRTRKLSVDLPGHKDEVYTVDWSVD 497

Query: 179 NKFLATAGMDSNVCIYETH 197
            K + + G D  V ++ TH
Sbjct: 498 GKRVCSGGKDKMVRLW-TH 515

 Score = 43.5 bits (101), Expect = 2e-08
 Identities = 20/78 (25%), Positives = 40/78 (51%), Gaps = 1/78 (1%)

Query: 472 NTRLLGHQKPVIHAQFSPN-GKMIVSSSFDKSIRVWSAADGKFLAVYRGHVGPVYKVAWS 530
           ++ + GH   ++ + F+P+    +V+ + D + R+W       +   +GH   V  V+WS
Sbjct: 136 SSAIAGHGSTILCSAFAPHTSSRMVTGAGDNTARIWDCDTQTPMHTLKGHYNWVLCVSWS 195

Query: 531 IDNNFFVSCSQDSTLKLW 548
            D     + S D+T++LW
Sbjct: 196 PDGEVIATGSMDNTIRLW 213

 Score = 36.6 bits (83), Expect = 3e-06
 Identities = 14/32 (43%), Positives = 20/32 (62%)

Query: 612 LPGHADAVYAVDWSNDGKFVASGGKDKVLKLW 643
           L GH + V  V WS DG+ +A+G  D  ++LW
Sbjct: 182 LKGHYNWVLCVSWSPDGEVIATGSMDNTIRLW 213

 Score = 35.0 bits (79), Expect = 8e-06
 Identities = 14/30 (46%), Positives = 19/30 (63%)

Query: 614 GHADAVYAVDWSNDGKFVASGGKDKVLKLW 643
           GH  +VY V WS+D + + S  KD  LK+W
Sbjct: 442 GHVASVYQVAWSSDCRLLVSCSKDTTLKVW 471

 Score = 26.9 bits (58), Expect = 0.002
 Identities = 10/32 (31%), Positives = 18/32 (56%), Gaps = 1/32 (3%)

Query: 612 LPGHADAVYAVDWSNDGKFVASGGKDKVLKLW 643
           + GH ++V  V W   G  + SG  D+ +++W
Sbjct: 274 MSGHTNSVSCVKWGGQG-LLYSGSHDRTVRVW 304

 Score = 20.4 bits (41), Expect = 0.21
 Identities = 10/39 (25%), Positives = 17/39 (43%), Gaps = 7/39 (17%)

Query: 612 LPGHADAVYAVDWS-------NDGKFVASGGKDKVLKLW 643
           L GH+  + ++ W             +AS  KD  +K+W
Sbjct: 225 LRGHSKWITSLSWEPIHLVKPGSKPRLASSSKDGTIKIW 263

 Score = 15.4 bits (28), Expect = 6.8
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 627 DGKFVAS 633
           DGKF+++
Sbjct: 433 DGKFIST 439

Lambda     K      H
   0.314    0.132    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1056
Number of extensions: 75
Number of successful extensions: 48
Number of sequences better than 10.0: 1
Number of HSP's gapped: 22
Number of HSP's successfully gapped: 22
Length of query: 645
Length of database: 515
Length adjustment: 36
Effective length of query: 609
Effective length of database: 479
Effective search space:   291711
Effective search space used:   291711
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 27 (15.2 bits)
S2: 27 (15.0 bits)