Query= PF13_0087 Pf
Annotation|Plasmodium_falciparum_Sanger|(protein coding) hypothetical
protein, conserved
(316 letters)
>YJL125C GCD14 SGDID:S000003661, Chr X from 186599-185448, reverse
complement, Verified ORF, "Subunit of tRNA
(1-methyladenosine) methyltransferase, with Gcd10p,
required for the modification of the adenine at position
58 in tRNAs, especially tRNAi-Met; first identified as a
negative regulator of GCN4 expression"
Length = 383
Score = 144 bits (363), Expect = 3e-39
Identities = 86/254 (33%), Positives = 142/254 (55%), Gaps = 24/254 (9%)
Query: 1 MIKEGNCVVIYSDEENIDLLKLTNDGIINNKKGRFLHKDIIGKEYGSKI-YDTLNKN--- 56
+IKEG+ +I+ +NI +++ ++ + N + G F HKDIIGK YGS+I T N
Sbjct: 12 LIKEGDLTLIWVSRDNIKPVRMHSEEVFNTRYGSFPHKDIIGKPYGSQIAIRTKGSNKFA 71
Query: 57 YIYVLKRSPELIVNCLKKKTQTLYEHDISFICLVCNALPNKKILEAGTGTGCLTYALANS 116
+++VL+ +PEL L +TQ +Y D S+I N P+ +++EAGTG+G ++A A S
Sbjct: 72 FVHVLQPTPELWTLSLPHRTQIVYTPDSSYIMQRLNCSPHSRVIEAGTGSGSFSHAFARS 131
Query: 117 VLPNGVIHTFEYNEERYKQVQQEFDDFENIRKNITFHHQDILNYNF-----DDFPLEYFD 171
V G + +FE++ RY+Q +EF + I N+T H+D+ F D E+ +
Sbjct: 132 V---GHLFSFEFHHIRYEQALEEFKEHGLIDDNVTITHRDVCQGGFLIKKGDTTSYEFGN 188
Query: 172 S----------IFLDMPNPWLCVDNMKKVLK--ERGVFVIFLPCIEQVYKILEALEKNSF 219
+ +FLD+P PW + ++ V+ E+ F PCIEQV K L+ LEK +
Sbjct: 189 NETAASLNANVVFLDLPAPWDAIPHLDSVISVDEKVGLCCFSPCIEQVDKTLDVLEKYGW 248
Query: 220 SDIETYELINKSWK 233
+D+E E+ + ++
Sbjct: 249 TDVEMVEIQGRQYE 262
Score = 23.9 bits (50), Expect = 0.007
Identities = 9/27 (33%), Positives = 16/27 (59%)
Query: 284 QKENKTHTGYLTIAKRCLNDEHEQPEI 310
+ E K+HT YLT A + +N + ++
Sbjct: 348 EAEIKSHTSYLTFAFKVVNRSRDDEKV 374
Lambda K H
0.321 0.140 0.421
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 323
Number of extensions: 20
Number of successful extensions: 9
Number of sequences better than 10.0: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 316
Length of database: 383
Length adjustment: 29
Effective length of query: 287
Effective length of database: 354
Effective search space: 101598
Effective search space used: 101598
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 23 (13.5 bits)
S2: 23 (13.5 bits)