>YBL076C ILS1 SGDID:S000000172, Chr II from 84259-81041, reverse
            complement, Verified ORF, "Cytoplasmic isoleucine-tRNA
            synthetase, target of the G1-specific inhibitor
            reveromycin A"
          Length = 1072

 Score =  711 bits (1836), Expect = 0.0
 Identities = 387/883 (43%), Positives = 550/883 (62%), Gaps = 46/883 (5%)

Query: 391  EVFAWTTTPWTLPSNLALCVNEHFIYLRVYHIESQRMFIFAECRLEWIMKELKWNVENIK 450
            ++ AWTTTPWTLPSNL+LCVN  F Y+++Y     R FI  E  ++ + K+ K   E  K
Sbjct: 218  QLVAWTTTPWTLPSNLSLCVNADFEYVKIYDETRDRYFILLESLIKTLYKKPK--NEKYK 275

Query: 451  IVNRFLGSELKELKYKPLFTNFYEKHNFKERAYKIIADDFVSDDAGTGIVHCAPSYGEDD 510
            IV +  GS+L  LKY+PLF  F E+  F E A+++I+DD+V+ D+GTGIVH AP++GE+D
Sbjct: 276  IVEKIKGSDLVGLKYEPLFPYFAEQ--FHETAFRVISDDYVTSDSGTGIVHNAPAFGEED 333

Query: 511  YRVCCKYGIIDPESNILIDPLDWNGYFTNEVEELKDMYIKDADNVIKKKLKENNRLMSNN 570
               C K G+I  E ++L + +D  G FT +V + + +Y+KDAD +I K L     L+  +
Sbjct: 334  NAACLKNGVIS-EDSVLPNAIDDLGRFTKDVPDFEGVYVKDADKLIIKYLTNTGNLLLAS 392

Query: 571  TIVHSYPFCWRSDTPLIYRAIPAWFVRVSNSTNKLVENNETTYWIPAHVKEKKFHNWIKD 630
             I HSYPFCWRSDTPL+YR++PAWFVRV N   +++++   ++W+P  +KEK+F NWI +
Sbjct: 393  QIRHSYPFCWRSDTPLLYRSVPAWFVRVKNIVPQMLDSVMKSHWVPNTIKEKRFANWIAN 452

Query: 631  AKDWCISRNRYWGTPIPIWCDEKMETVICVESIKHLEELSGIKNINDLHRHYIDNIEI-- 688
            A+DW +SRNRYWGTPIP+W  +  E V+CV SIK LEEL+G++NI DLHR  ID + I  
Sbjct: 453  ARDWNVSRNRYWGTPIPLWVSDDFEEVVCVGSIKELEELTGVRNITDLHRDVIDKLTIPS 512

Query: 689  KNPKGDTYPKLKRIPEVFDCWFESGSMPYAKVHYPFSTETNDFHKIFPADFIAEGLDQTR 748
            K  KGD    LKRI EVFDCWFESGSMPYA  HYPF   T  F +  PA+FI+EGLDQTR
Sbjct: 513  KQGKGD----LKRIEEVFDCWFESGSMPYASQHYPFE-NTEKFDERVPANFISEGLDQTR 567

Query: 749  GWFYTLLVISTLLFNKAPFKNLICNGLVLASDGKKMSKRLKNYPDPLYILNKYGADSLRL 808
            GWFYTL V+ T LF   P+KN+I +G+VLA+DG+KMSK LKNYPDP  +LNKYGAD+LRL
Sbjct: 568  GWFYTLAVLGTHLFGSVPYKNVIVSGIVLAADGRKMSKSLKNYPDPSIVLNKYGADALRL 627

Query: 809  YLINSVAVRAENLKFQEKGVNEIVKSFILPYYHSFRFFSQEVTRYETTNKMQFLFNTDYI 868
            YLINS  ++AE+LKF+E+GV E+V   +LP+++SF+F   ++   +  + + F +  D  
Sbjct: 628  YLINSPVLKAESLKFKEEGVKEVVSKVLLPWWNSFKFLDGQIALLKKMSNIDFQY--DDS 685

Query: 869  YKNDNIMDQWIFSSLQSLINSVHTEMKAYKLYNVLPKLLNFIENLTNWYIRLNRDRMRGM 928
             K+DN+MD+WI +S+QSL+  +H EM  YKLY V+PKLLNFI+ LTNWYIR NR R++G 
Sbjct: 686  VKSDNVMDRWILASMQSLVQFIHEEMGQYKLYTVVPKLLNFIDELTNWYIRFNRRRLKGE 745

Query: 929  LGKENTHQSLNVLCRTLYLFTIIMAPFTPFISEYIYQFLKNIKYXXXXXXXXXXXXXXXX 988
             G E+  ++LN L   L+ F   MAPFTPF+SE IY  LK  +Y                
Sbjct: 746  NGVEDCLKALNSLFDALFTFVRAMAPFTPFLSESIYLRLK--EYIPEAVLAKYGKDG--- 800

Query: 989  XXXXDLNRNDIHKSVHFIMLPQVDDKYIIKYEFIE-LIEHMKDVILLGRNLREKRKTPNK 1047
                        +SVHF+  P V  +Y    E IE  +  M+ VI LGRN+REK+    K
Sbjct: 801  ------------RSVHFLSYPVVKKEYF--DEAIETAVSRMQSVIDLGRNIREKKTISLK 846

Query: 1048 KPLKSLTILHKNESFFKHFDRISNYIKEELNILNVEYSNDIS--CLNFSAIPNFKKLGVK 1105
             PLK+L ILH +ES+ K  + + NYI EELN+ +V  ++D +   + + A+ ++  LG K
Sbjct: 847  TPLKTLVILHSDESYLKDVEALKNYIIEELNVRDVVITSDEAKYGVEYKAVADWPVLGKK 906

Query: 1106 LGYXXXXXXXXXXXXXAEDIKLFQQNKQIIIDNILLEQDDI--IIQMNHNIQNDNTEAIS 1163
            L               +E ++ + ++ ++ +  I L + D+  I  +  +      E  +
Sbjct: 907  LKKDAKKVKDALPSVTSEQVREYLESGKLEVAGIELVKGDLNAIRGLPESAVQAGQETRT 966

Query: 1164 NNYITILMDFTADQQLENMASAREICNHIQKIRKNLSLNQNSPVLMHIYIYDQT--FKNH 1221
            +  + I+MD     +L++   ARE+ N IQK+RK   L     VL+   +   T  F+  
Sbjct: 967  DQDVLIIMDTNIYSELKSEGLARELVNRIQKLRKKCGLEATDDVLVEYELVKDTIDFEAI 1026

Query: 1222 MLNENEYIKKCLRRNLNIIDLEK------DVQNLTDKFFEEKI 1258
            +    + + K  R ++   D  K      + Q++ D  F+ K+
Sbjct: 1027 VKEHFDMLSKTCRSDIAKYDGSKTDPIGDEEQSINDTIFKLKV 1069

 Score =  241 bits (615), Expect = 3e-67
 Identities = 103/198 (52%), Positives = 138/198 (69%)

Query: 96  EEEERILKYWEDIDAFNLSNELSKNKKPYIFYDGPPFATGLPHYGHLLAGIIKDCVTRYF 155
           +EEE++L  W++IDAF+ S EL+K+K  + F+DGPPFATG PHYGH+LA  IKD V RY 
Sbjct: 12  KEEEKVLSLWDEIDAFHTSLELTKDKPEFSFFDGPPFATGTPHYGHILASTIKDIVPRYA 71

Query: 156 YQSNFYVERRFGWDCHGLPVEYXXXXXXXXXXREDILNMGIDVYNEKCRGIVLKYSKEWV 215
             +  +VERRFGWD HG+P+E+          ++D+   G++ YN +CR IV+ Y+ +W 
Sbjct: 72  TMTGHHVERRFGWDTHGVPIEHIIDKKLGITGKDDVFKYGLENYNNECRSIVMTYASDWR 131

Query: 216 ATVQRIGRWIDFKNDYKTMDKSFMESVWWVFSELYKNNYIYKSFKVMPYSCKCNTPISNF 275
            T+ R+GRWIDF NDYKTM  SFMES WW F +L++   +Y+ FKVMPYS    TP+SNF
Sbjct: 132 KTIGRLGRWIDFDNDYKTMYPSFMESTWWAFKQLHEKGQVYRGFKVMPYSTGLTTPLSNF 191

Query: 276 ELNLNYKDTPDPSIIISF 293
           E   NYKD  DP++ I F
Sbjct: 192 EAQQNYKDVNDPAVTIGF 209

 Score = 20.0 bits (40), Expect = 1.2
 Identities = 14/61 (22%), Positives = 25/61 (40%), Gaps = 9/61 (14%)

Query: 863 FNTDYIYKNDNIMDQ--WIFSSLQSLINSVHTEMKAYKLYNVLPKLLNFIENLTNWYIRL 920
           F+ DY     + M+   W F  L       H + + Y+ + V+P        L+N+  + 
Sbjct: 143 FDNDYKTMYPSFMESTWWAFKQL-------HEKGQVYRGFKVMPYSTGLTTPLSNFEAQQ 195

Query: 921 N 921
           N
Sbjct: 196 N 196

 Score = 20.0 bits (40), Expect = 1.2
 Identities = 6/17 (35%), Positives = 14/17 (82%)

Query: 276 ELNLNYKDTPDPSIIIS 292
           +++ + K+ PDPSI+++
Sbjct: 602 KMSKSLKNYPDPSIVLN 618

 Score = 18.5 bits (36), Expect = 3.4
 Identities = 7/22 (31%), Positives = 12/22 (54%)

Query: 189  EDILNMGIDVYNEKCRGIVLKY 210
            E I+    D+ ++ CR  + KY
Sbjct: 1024 EAIVKEHFDMLSKTCRSDIAKY 1045

 Score = 18.1 bits (35), Expect = 4.5
 Identities = 13/61 (21%), Positives = 27/61 (44%), Gaps = 5/61 (8%)

Query: 225 IDFKNDYKT-----MDKSFMESVWWVFSELYKNNYIYKSFKVMPYSCKCNTPISNFELNL 279
           IDF+ D        MD+  + S+  +   +++    YK + V+P        ++N+ +  
Sbjct: 678 IDFQYDDSVKSDNVMDRWILASMQSLVQFIHEEMGQYKLYTVVPKLLNFIDELTNWYIRF 737

Query: 280 N 280
           N
Sbjct: 738 N 738

Lambda     K      H
   0.322    0.139    0.428 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 3574
Number of extensions: 183
Number of successful extensions: 16
Number of sequences better than 10.0: 1
Number of HSP's gapped: 7
Number of HSP's successfully gapped: 7
Length of query: 1272
Length of database: 1072
Length adjustment: 47
Effective length of query: 1225
Effective length of database: 1025
Effective search space:  1255625
Effective search space used:  1255625
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 33 (18.2 bits)
S2: 33 (17.3 bits)