>YDL058W USO1 SGDID:S000002216, Chr IV from 345665-351037, Verified
            ORF, "Essential protein involved in intracellular protein
            transport, coiled-coil protein necessary for transport
            from ER to Golgi; required for assembly of the
            ER-to-Golgi SNARE complex"
          Length = 1790

 Score = 47.0 bits (110), Expect = 3e-08
 Identities = 93/482 (19%), Positives = 162/482 (33%), Gaps = 70/482 (14%)

Query: 370  NKNISLYRNLCY-LENKNFNLRKELFHYKQKILFYKTYVNDMRDKLKHNIIQLYNQYHQN 428
            N+N+   +  C  L  +  ++ KEL  YK +   +   V  + +KLK     L N Y   
Sbjct: 919  NENLEEMKIQCNNLSKEKEHISKELVEYKSRFQSHDNLVAKLTEKLK----SLANNYKDM 974

Query: 429  KNENTEIMTSVDETFXXXXXXXXXXXXXXXKHISYSKKNICDNNTKNNSLNQNYDFIKNQ 488
            + EN  ++ +V+E+                       KN  +++ + ++L    D +  +
Sbjct: 975  QAENESLIKAVEES-----------------------KN--ESSIQLSNLQNKIDSMSQE 1009

Query: 489  KSFFGLNNSDFNNILKTNNSKNDHNEKIIMQTSTKLNELNVEKKNIEEVKDKYLDPLEKD 548
            K  F +                   EK I Q    +++L   K+ I    D   D  E  
Sbjct: 1010 KENFQIERGSI--------------EKNIEQLKKTISDLEQTKEEIISKSDSSKDEYESQ 1055

Query: 549  IPLVDLNIH---NKNDMYYFEIKELIHKRKIELD------KKLKGDINVNINTSHHNINS 599
            I L+   +      ND    +I EL   R+ EL+      K LK ++   + TS   +  
Sbjct: 1056 ISLLKEKLETATTANDENVNKISELTKTRE-ELEAELAAYKNLKNELETKLETSEKALKE 1114

Query: 600  GCNYVKGREEDNSKNKSTLSGVIKNNILENTDNLTYINKSNNS-KPEHXXXXXXXXXXXX 658
                VK  EE   + K  L    +    E    L  +  +  S + EH            
Sbjct: 1115 ----VKENEEHLKEEKIQL----EKEATETKQQLNSLRANLESLEKEHEDLAAQLKKYEE 1166

Query: 659  XXXXXXXXXXXDFYVQNKNILXXXXXXXXXXXXXXXXXXXXXXXXXTNQHFNNTSNDILK 718
                       +    N  I                          T++  +N     + 
Sbjct: 1167 QIANKERQYNEEISQLNDEITSTQQENESIKKKNDELEGEVKAMKSTSEEQSNLKKSEID 1226

Query: 719  HYNQSIPCVNRRNE-------HSINLNEDKNINQKETNDEDTKNDDINMNVEDYHDITLG 771
              N  I  + ++NE        SI   E + +  KE  DE    +     +ED    +  
Sbjct: 1227 ALNLQIKELKKKNETNEASLLESIKSVESETVKIKELQDECNFKEKEVSELEDKLKASED 1286

Query: 772  DSHQNFIYNIEEENIKQDLPEETCKKEDNINDYFNEKKNKYQDNIKCTSLKETGKDVHKI 831
             + +      E E IK++L  +T + +  +    N  K K +   + + LK+T  +  K 
Sbjct: 1287 KNSKYLELQKESEKIKEELDAKTTELKIQLEKITNLSKAKEKSESELSRLKKTSSEERKN 1346

Query: 832  QE 833
             E
Sbjct: 1347 AE 1348

 Score = 33.5 bits (75), Expect = 4e-04
 Identities = 66/346 (19%), Positives = 125/346 (36%), Gaps = 71/346 (20%)

Query: 316  KDELIMQNKLLKEKILQIETFILRDPFLVEKLCPIHEXXXXXXXXXXXXXXYQNNKN--- 372
            KDE   Q  LLKEK   +ET    +   V K   I E              Y+N KN   
Sbjct: 1049 KDEYESQISLLKEK---LETATTANDENVNK---ISELTKTREELEAELAAYKNLKNELE 1102

Query: 373  ----------ISLYRNLCYLENKNFNLRKELFHYKQKILFYKTYVNDMRDKLKHNIIQLY 422
                        +  N  +L+ +   L KE    KQ++   +  +  +  + +    QL 
Sbjct: 1103 TKLETSEKALKEVKENEEHLKEEKIQLEKEATETKQQLNSLRANLESLEKEHEDLAAQLK 1162

Query: 423  NQYHQNKNENTEIMTSVDETFXXXXXXXXXXXXXXXKHISYSKKNICDNNTKNNSLNQNY 482
                Q  N+  +                        + IS     I     +N S+ +  
Sbjct: 1163 KYEEQIANKERQ----------------------YNEEISQLNDEITSTQQENESIKKKN 1200

Query: 483  DFIKNQKSFFGLNNSDFNNILKTN-----------NSKNDHNEKIIMQT-------STKL 524
            D ++ +       + + +N+ K+              KN+ NE  ++++       + K+
Sbjct: 1201 DELEGEVKAMKSTSEEQSNLKKSEIDALNLQIKELKKKNETNEASLLESIKSVESETVKI 1260

Query: 525  NEL----NVEKKNIEEVKDKYLDPLEKDIPLVDLNIHNKNDMYYFEIKELIHKRKIELDK 580
             EL    N ++K + E++DK     +K+   ++L   ++      +IKE +  +  EL  
Sbjct: 1261 KELQDECNFKEKEVSELEDKLKASEDKNSKYLELQKESE------KIKEELDAKTTEL-- 1312

Query: 581  KLKGDINVNINTSHHNINSGCNYVKGREEDNSKNKSTLSGVIKNNI 626
            K++ +   N++ +     S  + +K    +  KN       +KN I
Sbjct: 1313 KIQLEKITNLSKAKEKSESELSRLKKTSSEERKNAEEQLEKLKNEI 1358

 Score = 32.3 bits (72), Expect = 8e-04
 Identities = 39/171 (22%), Positives = 72/171 (42%), Gaps = 28/171 (16%)

Query: 501  NILKTNNSKNDHNEKIIMQTSTKLNELNVEKKN-IEEVKDKYLDPLEK----DIPLVDLN 555
            N LK     N  +E  + + S   +EL  EK+N I+ ++D+ L   +K    D  L+ + 
Sbjct: 1401 NELKAKEIDNTRSE--LEKVSLSNDELLEEKQNTIKSLQDEILSYKDKITRNDEKLLSIE 1458

Query: 556  IHNKNDMYYFE-------------------IKELIHKRKIELDK--KLKGDINVNINTSH 594
              NK D+   +                   ++E   K K EL+K  ++   +   I ++ 
Sbjct: 1459 RDNKRDLESLKEQLRAAQESKAKVEEGLKKLEEESSKEKAELEKSKEMMKKLESTIESNE 1518

Query: 595  HNINSGCNYVKGREEDNSKNKSTLSGVIKNNILENTDNLTYINKSNNSKPE 645
              + S    ++  +E   ++K +    IKN   E +D ++ IN+S     E
Sbjct: 1519 TELKSSMETIRKSDEKLEQSKKSAEEDIKNLQHEKSDLISRINESEKDIEE 1569

 Score = 28.5 bits (62), Expect = 0.012
 Identities = 26/124 (20%), Positives = 51/124 (41%), Gaps = 8/124 (6%)

Query: 717  LKHYNQSIPCVNRR-NEHSINLNEDKNINQKETNDEDTKNDDINMNVEDYHDITLGDSHQ 775
            LK Y + I    R+ NE    LN++    Q+E      KND++   V+     +     Q
Sbjct: 1161 LKKYEEQIANKERQYNEEISQLNDEITSTQQENESIKKKNDELEGEVKAMKSTS---EEQ 1217

Query: 776  NFIYNIEEENIKQDLPEETCKKEDNINDYFNEKKNKYQDNIKCTSLKE----TGKDVHKI 831
            + +   E + +   + E   K E N        K+   + +K   L++      K+V ++
Sbjct: 1218 SNLKKSEIDALNLQIKELKKKNETNEASLLESIKSVESETVKIKELQDECNFKEKEVSEL 1277

Query: 832  QENI 835
            ++ +
Sbjct: 1278 EDKL 1281

 Score = 25.4 bits (54), Expect = 0.10
 Identities = 23/132 (17%), Positives = 55/132 (41%), Gaps = 8/132 (6%)

Query: 709  FNNTSNDILKHYNQSIPCVNRRNEHSINLNEDKNINQKETNDEDTKNDDINMNVEDYHDI 768
            + N  N++      S   +    E+  +L E+K   +KE  +   + + +  N+E     
Sbjct: 1094 YKNLKNELETKLETSEKALKEVKENEEHLKEEKIQLEKEATETKQQLNSLRANLESLEKE 1153

Query: 769  TLGDSHQNFIYNIEEENIKQDLPEETCKKEDNI------NDYFNEKKNKYQDNIKCTSLK 822
                + Q   Y  +  N ++   EE  +  D I      N+   +K ++ +  +K  ++K
Sbjct: 1154 HEDLAAQLKKYEEQIANKERQYNEEISQLNDEITSTQQENESIKKKNDELEGEVK--AMK 1211

Query: 823  ETGKDVHKIQEN 834
             T ++   ++++
Sbjct: 1212 STSEEQSNLKKS 1223

 Score = 25.0 bits (53), Expect = 0.13
 Identities = 31/132 (23%), Positives = 56/132 (42%), Gaps = 19/132 (14%)

Query: 705  TNQHFNNTSNDILKHYNQSIPCVNRRNEHSINLNEDKNINQKETNDEDTKNDDINMNVED 764
            +NQ         LK   Q +    ++ + S    E++    ++   E ++ D+  M +E 
Sbjct: 1628 SNQEEKELLTSRLKELEQELDSTQQKAQKS---EEERRAEVRKFQVEKSQLDEKAMLLET 1684

Query: 765  -YHDITLGDSHQNFIYNIEEENIKQDLPEETCKKEDNINDYFNEKKNKYQDNIKC--TSL 821
             Y+D+             +E+  K+D  E+T KK  +      EK  K  DN+K   + L
Sbjct: 1685 KYNDLVN-----------KEQAWKRD--EDTVKKTTDSQRQEIEKLAKELDNLKAENSKL 1731

Query: 822  KETGKDVHKIQE 833
            KE  +D  +I +
Sbjct: 1732 KEANEDRSEIDD 1743

 Score = 24.6 bits (52), Expect = 0.17
 Identities = 15/57 (26%), Positives = 28/57 (49%), Gaps = 1/57 (1%)

Query: 1056 IIENVRKYEEYIDDKNDRINTHTIDEKNVISIEEDNNGK-QIMKQESRELNDNEKRE 1111
            I    R+Y E I   ND I +   + +++    ++  G+ + MK  S E ++ +K E
Sbjct: 1168 IANKERQYNEEISQLNDEITSTQQENESIKKKNDELEGEVKAMKSTSEEQSNLKKSE 1224

 Score = 23.5 bits (49), Expect = 0.39
 Identities = 13/48 (27%), Positives = 25/48 (52%), Gaps = 1/48 (2%)

Query: 2254 KNNIQSFYSDLDKLLLNIFNAEQKYFTEEIETNLRKIKSFKKILKQMK 2301
            + N ++   + DK  +N    E K   E+I   + +IK+  + L++MK
Sbjct: 880  EENCKNLQKEKDKSNVN-HQKETKSLKEDIAAKITEIKAINENLEEMK 926

 Score = 22.7 bits (47), Expect = 0.66
 Identities = 9/23 (39%), Positives = 13/23 (56%)

Query: 145 YLKSQSRIHNNKYIKDIRKKQSK 167
           ++  QSR+ N KY   +  KQ K
Sbjct: 100 WISQQSRLQNGKYPSPLVMKQEK 122

 Score = 22.7 bits (47), Expect = 0.66
 Identities = 11/34 (32%), Positives = 23/34 (67%), Gaps = 1/34 (2%)

Query: 2282 EIETNLRKI-KSFKKILKQMKDKETFLKNIKYEK 2314
            E+++++  I KS +K+ +  K  E  +KN+++EK
Sbjct: 1520 ELKSSMETIRKSDEKLEQSKKSAEEDIKNLQHEK 1553

 Score = 22.3 bits (46), Expect = 0.87
 Identities = 16/54 (29%), Positives = 28/54 (51%), Gaps = 2/54 (3%)

Query: 2519 EENILTDKINKTKSFYLYKYDYFGELSIINEQLRTTRCEAHTNCFLLAIDKISF 2572
            E++ILT ++++T    +Y   YF  + + NE +   R     +     I+KISF
Sbjct: 678  EDSILTPELDETGLPKVYFSTYF--IQLFNENIYRIRTALSHDPDEEPINKISF 729

 Score = 22.3 bits (46), Expect = 0.87
 Identities = 9/18 (50%), Positives = 12/18 (66%)

Query: 893  DITNDTYKNTEISKKNDE 910
            +IT+   +N  I KKNDE
Sbjct: 1185 EITSTQQENESIKKKNDE 1202

 Score = 21.9 bits (45), Expect = 1.1
 Identities = 19/82 (23%), Positives = 39/82 (47%), Gaps = 4/82 (4%)

Query: 1964 RNISEEFMSIVNKIPILKNFPNMKKISLSMNVKKSMFEKNKIVIRKHDDPLQFFILKEGK 2023
            + + EE  S   K  + K+   MKK+  ++   ++  + +   IRK D+ L+    K+  
Sbjct: 1487 KKLEEE--SSKEKAELEKSKEMMKKLESTIESNETELKSSMETIRKSDEKLE--QSKKSA 1542

Query: 2024 INIFYHTSKTKNNPINTINENQ 2045
                 +    K++ I+ INE++
Sbjct: 1543 EEDIKNLQHEKSDLISRINESE 1564

 Score = 21.2 bits (43), Expect = 1.9
 Identities = 12/59 (20%), Positives = 30/59 (50%), Gaps = 5/59 (8%)

Query: 741  KNINQKETNDEDTKNDDINMNVEDYHDITLGDSHQNFIYNIEEENIKQDLPEETCKKED 799
            +N   KE N++ ++ DD+ + V D     L + +  +   +++  ++    EE  +++D
Sbjct: 1727 ENSKLKEANEDRSEIDDLMLLVTD-----LDEKNAKYRSKLKDLGVEISSDEEDDEEDD 1780

 Score = 20.8 bits (42), Expect = 2.5
 Identities = 8/35 (22%), Positives = 18/35 (51%)

Query: 504 KTNNSKNDHNEKIIMQTSTKLNELNVEKKNIEEVK 538
           K+N +     + +    + K+ E+    +N+EE+K
Sbjct: 892 KSNVNHQKETKSLKEDIAAKITEIKAINENLEEMK 926

 Score = 20.4 bits (41), Expect = 3.3
 Identities = 19/82 (23%), Positives = 37/82 (45%), Gaps = 3/82 (3%)

Query: 2476 KYQDKPDFFYIIKKGIVTVKTDFKEDTNQCVTNQKGVEEKINEEENILTDKINKTKSFYL 2535
            ++Q   +    + + + ++  ++K+   +  +  K VEE  NE    L++  NK  S   
Sbjct: 949  RFQSHDNLVAKLTEKLKSLANNYKDMQAENESLIKAVEESKNESSIQLSNLQNKIDSMSQ 1008

Query: 2536 YKYDYFGELSIIN---EQLRTT 2554
             K ++  E   I    EQL+ T
Sbjct: 1009 EKENFQIERGSIEKNIEQLKKT 1030

 Score = 20.4 bits (41), Expect = 3.3
 Identities = 15/63 (23%), Positives = 30/63 (47%)

Query: 121  KNISLERSNVELNNQLLNKDNISQYLKSQSRIHNNKYIKDIRKKQSKSNKSLENIKGYNN 180
            K +S+ER N      L  +   +Q  K++      K  ++  K++++  KS E +K   +
Sbjct: 1453 KLLSIERDNKRDLESLKEQLRAAQESKAKVEEGLKKLEEESSKEKAELEKSKEMMKKLES 1512

Query: 181  VIK 183
             I+
Sbjct: 1513 TIE 1515

 Score = 20.0 bits (40), Expect = 4.3
 Identities = 10/34 (29%), Positives = 18/34 (52%), Gaps = 8/34 (23%)

Query: 1451 NLNEFFNAVIKQNKLYISLNEFIYFFSANEKMTK 1484
            NLN F        KL+ + + F++FF  + K ++
Sbjct: 506  NLNPF--------KLFFTTDIFMFFFQQDHKYSE 531

 Score = 20.0 bits (40), Expect = 4.3
 Identities = 15/78 (19%), Positives = 32/78 (41%), Gaps = 1/78 (1%)

Query: 736  NLNEDKNINQKETNDEDTKNDDINMNVEDYHDITLGDSHQNFIYNIEEENIKQDLPEETC 795
            N  E   IN +E     +K +DI   ++D       +  +  +     + ++Q+L + T 
Sbjct: 1593 NAQEKIRINAEENTVLKSKLEDIERELKDKQAEIKSNQEEKELLTSRLKELEQEL-DSTQ 1651

Query: 796  KKEDNINDYFNEKKNKYQ 813
            +K     +    +  K+Q
Sbjct: 1652 QKAQKSEEERRAEVRKFQ 1669

 Score = 19.2 bits (38), Expect = 7.3
 Identities = 8/25 (32%), Positives = 15/25 (60%)

Query: 2507 TNQKGVEEKINEEENILTDKINKTK 2531
            T++K ++E    EE++  +KI   K
Sbjct: 1107 TSEKALKEVKENEEHLKEEKIQLEK 1131

 Score = 19.2 bits (38), Expect = 7.3
 Identities = 7/30 (23%), Positives = 19/30 (63%)

Query: 1986 MKKISLSMNVKKSMFEKNKIVIRKHDDPLQ 2015
            +KK+    + +K+  EK+K +++K +  ++
Sbjct: 1486 LKKLEEESSKEKAELEKSKEMMKKLESTIE 1515

 Score = 18.9 bits (37), Expect = 9.6
 Identities = 8/19 (42%), Positives = 12/19 (63%)

Query: 2513 EEKINEEENILTDKINKTK 2531
            E + NEE + L D+I  T+
Sbjct: 1172 ERQYNEEISQLNDEITSTQ 1190

Lambda     K      H
   0.316    0.135    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 11,289
Number of extensions: 654
Number of successful extensions: 28
Number of sequences better than 10.0: 1
Number of HSP's gapped: 25
Number of HSP's successfully gapped: 25
Length of query: 2691
Length of database: 1790
Length adjustment: 56
Effective length of query: 2635
Effective length of database: 1734
Effective search space:  4569090
Effective search space used:  4569090
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (19.8 bits)
S2: 37 (18.9 bits)