>YPL146C NOP53 SGDID:S000006067, Chr XVI from 277528-276161, reverse
           complement, Verified ORF, "Nucleolar protein; involved
           in biogenesis of the 60S subunit of the ribosome;
           interacts with rRNA processing factors Cbf5p and Nop2p;
           null mutant is viable but growth is severely impaired"
          Length = 455

 Score = 32.7 bits (73), Expect = 1e-05
 Identities = 17/71 (23%), Positives = 35/71 (49%)

Query: 55  NNKCIKKERIEPNELKNEVNIEGDQVKQNEQNHDDDDVINENEKAVLEFYDEKIDNYDEE 114
           N K  K +R +    + +V ++ +  +  ++  D ++VIN  E  +L   +   +   + 
Sbjct: 316 NKKKTKYQRNKAKRHEEKVKLQQELKELRQRVKDLEEVINSEETEILSAIESDSNKVKKS 375

Query: 115 YVNKKYRFGTK 125
             NKK++ GTK
Sbjct: 376 KKNKKHKLGTK 386

 Score = 25.0 bits (53), Expect = 0.003
 Identities = 10/28 (35%), Positives = 17/28 (60%)

Query: 176 NSKRYEENDKDHGNNENKQNNESDKSLK 203
           N +  EE +++   NE+ Q + SDK +K
Sbjct: 280 NEEEEEEEEENENENESTQCSGSDKEIK 307

 Score = 23.1 bits (48), Expect = 0.010
 Identities = 20/81 (24%), Positives = 36/81 (44%), Gaps = 3/81 (3%)

Query: 42  LNITCSDEKFINDNNKCIKK--ERIEPNELKNEVNIEGDQVKQNEQNHDDDDVINENEKA 99
           +N    +EK   D    ++K  ERI       + N E +     E+  ++++  NENE  
Sbjct: 238 INKEYKEEKAREDERIALEKYKERIRHLMETLDDNEEEESSSNEEEEEEEEENENENEST 297

Query: 100 VLEFYDEKID-NYDEEYVNKK 119
                D++I  + ++   NKK
Sbjct: 298 QCSGSDKEIKLSINKPVKNKK 318

 Score = 21.6 bits (44), Expect = 0.029
 Identities = 20/68 (29%), Positives = 27/68 (39%), Gaps = 17/68 (25%)

Query: 64  IEPNELKNEVNIEGDQVKQNEQNHDDDDVIN---------ENEKAVLEFYDEKI------ 108
           IEP  +K    I       N  N    ++IN         E+E+  LE Y E+I      
Sbjct: 209 IEPIAVKEFTEIPHAGKSYNPNNKAWSELINKEYKEEKAREDERIALEKYKERIRHLMET 268

Query: 109 --DNYDEE 114
             DN +EE
Sbjct: 269 LDDNEEEE 276

 Score = 19.6 bits (39), Expect = 0.11
 Identities = 16/57 (28%), Positives = 25/57 (43%), Gaps = 10/57 (17%)

Query: 178 KRYEENDKDHGNNENKQNNESDKSLKYHAVFCINCNNHIAYFEIQERIFHFFDVLPD 234
           K Y  N+K      NK+  E +K+ +         +  IA  + +ERI H  + L D
Sbjct: 225 KSYNPNNKAWSELINKEYKE-EKARE---------DERIALEKYKERIRHLMETLDD 271

 Score = 19.2 bits (38), Expect = 0.14
 Identities = 6/18 (33%), Positives = 13/18 (72%)

Query: 67 NELKNEVNIEGDQVKQNE 84
          N+    V++EGD++ +N+
Sbjct: 57 NDALFHVDVEGDEILKNK 74

 Score = 17.7 bits (34), Expect = 0.41
 Identities = 14/46 (30%), Positives = 22/46 (47%), Gaps = 7/46 (15%)

Query: 56  NKCIKKERIEPNELKNEVNIEGDQVKQNEQ----NHDDDDVINENE 97
           NK IK+++I+      E+    D VK N +    NH  +   N N+
Sbjct: 73  NKLIKRKQIKKVLKSKEIL---DAVKTNSKIAALNHHKNSSGNPNK 115

 Score = 17.3 bits (33), Expect = 0.54
 Identities = 15/53 (28%), Positives = 22/53 (41%), Gaps = 4/53 (7%)

Query: 73  VNIEGDQVKQNEQNHDDDDVINENEKAVLEF----YDEKIDNYDEEYVNKKYR 121
           ++IE   VK+  +        N N KA  E     Y E+    DE    +KY+
Sbjct: 207 LDIEPIAVKEFTEIPHAGKSYNPNNKAWSELINKEYKEEKAREDERIALEKYK 259

 Score = 16.9 bits (32), Expect = 0.70
 Identities = 8/35 (22%), Positives = 18/35 (51%)

Query: 64 IEPNELKNEVNIEGDQVKQNEQNHDDDDVINENEK 98
          +E +E+     I+  Q+K+  ++ +  D +  N K
Sbjct: 65 VEGDEILKNKLIKRKQIKKVLKSKEILDAVKTNSK 99

 Score = 16.9 bits (32), Expect = 0.70
 Identities = 5/19 (26%), Positives = 11/19 (57%)

Query: 181 EENDKDHGNNENKQNNESD 199
           EE+  +    E ++ NE++
Sbjct: 275 EESSSNEEEEEEEEENENE 293

 Score = 14.2 bits (25), Expect = 4.6
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 177 SKRYEENDK 185
           +KR+EE  K
Sbjct: 327 AKRHEEKVK 335

 Score = 13.9 bits (24), Expect = 5.9
 Identities = 4/11 (36%), Positives = 9/11 (81%)

Query: 100 VLEFYDEKIDN 110
           V ++ ++KID+
Sbjct: 33  VEQYMEKKIDH 43

Lambda     K      H
   0.316    0.134    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 358
Number of extensions: 26
Number of successful extensions: 19
Number of sequences better than 10.0: 1
Number of HSP's gapped: 15
Number of HSP's successfully gapped: 13
Length of query: 234
Length of database: 455
Length adjustment: 28
Effective length of query: 206
Effective length of database: 427
Effective search space:    87962
Effective search space used:    87962
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 23 (13.4 bits)
S2: 23 (13.5 bits)