>YAL035W FUN12 SGDID:S000000033, Chr I from 76428-79436, Verified
           ORF, "GTPase, required for general translation
           initiation by promoting Met-tRNAiMet binding to
           ribosomes and ribosomal subunit joining; homolog of
           bacterial IF2"
          Length = 1002

 Score = 35.4 bits (80), Expect = 1e-05
 Identities = 38/209 (18%), Positives = 81/209 (38%), Gaps = 31/209 (14%)

Query: 185 KKEKEYTDIQRDKRKHKRSLSQKSDSYKKRPFNKRKTSIERSLSNKRYDEKTNKRFNKNF 244
           K+EK+  + ++D +   +S  +K    K++   K+K    R  + ++  ++ NK  NK  
Sbjct: 72  KQEKKVIEEKKDGKPILKSKKEKEKEKKEKEKQKKKEQAARKKAQQQAQKEKNKELNKQ- 130

Query: 245 RTDIYNRKHSKDIGYKHNNSYSSSDDKYHNGRSLSSSDISYQHXXXXXXXXXXXXHDDSS 304
             ++      K    K   S   SD    + +                           +
Sbjct: 131 --NVEKAAAEKAAAEKSQKSKGESDKPSASAKK-------------------------PA 163

Query: 305 SEISSHTYASRYDKERKRFRKRDINLERHKERGYINRDKE--GKHIRRDKEGKHIRRDKE 362
            ++ +   A R   E K+  +    LER +E      ++E      +  +E K  +++KE
Sbjct: 164 KKVPAGLAALRRQLELKKQLEEQEKLEREEEERLEKEEEERLANEEKMKEEAKAAKKEKE 223

Query: 363 E-KHIRRDKEGKHIRGEQERRHQIRNKEK 390
           + K  +R  EGK +  +Q+   ++  + +
Sbjct: 224 KAKREKRKAEGKLLTRKQKEEKKLLERRR 252

 Score = 31.2 bits (69), Expect = 2e-04
 Identities = 17/59 (28%), Positives = 34/59 (57%), Gaps = 2/59 (3%)

Query: 331 ERHKERGYINRDKEGKHIRRDKEGKHIRRDKEEKHIRRDKEGKHIRGEQERRHQIRNKE 389
           ++ +E+  I   K+GK I + K+ K   ++K+EK  ++ KE    +  Q++  + +NKE
Sbjct: 70  KKKQEKKVIEEKKDGKPILKSKKEK--EKEKKEKEKQKKKEQAARKKAQQQAQKEKNKE 126

 Score = 31.2 bits (69), Expect = 2e-04
 Identities = 20/95 (21%), Positives = 46/95 (48%), Gaps = 3/95 (3%)

Query: 166 KDKEHVSHQNEHNINNVNLKKEKEYTDIQRDKRKHKRSLSQK---SDSYKKRPFNKRKTS 222
           K ++ V  + +     +  KKEKE    +++K+K K   ++K     + K++     K +
Sbjct: 72  KQEKKVIEEKKDGKPILKSKKEKEKEKKEKEKQKKKEQAARKKAQQQAQKEKNKELNKQN 131

Query: 223 IERSLSNKRYDEKTNKRFNKNFRTDIYNRKHSKDI 257
           +E++ + K   EK+ K   ++ +     +K +K +
Sbjct: 132 VEKAAAEKAAAEKSQKSKGESDKPSASAKKPAKKV 166

 Score = 30.4 bits (67), Expect = 4e-04
 Identities = 19/58 (32%), Positives = 30/58 (51%), Gaps = 3/58 (5%)

Query: 341 RDKEGKHIRRDKEGKHIRRDKEEKHIRRDKEGKHIRGEQERR---HQIRNKEKYRDRN 395
           + +E K I   K+GK I + K+EK   + ++ K  + EQ  R    Q   KEK ++ N
Sbjct: 71  KKQEKKVIEEKKDGKPILKSKKEKEKEKKEKEKQKKKEQAARKKAQQQAQKEKNKELN 128

 Score = 26.6 bits (57), Expect = 0.005
 Identities = 14/48 (29%), Positives = 28/48 (58%), Gaps = 3/48 (6%)

Query: 355 KHIRRDKEEKHIRRDKEGKHI---RGEQERRHQIRNKEKYRDRNDHKK 399
           K  ++ +E+K I   K+GK I   + E+E+  + + K+K +++   KK
Sbjct: 67  KQSKKKQEKKVIEEKKDGKPILKSKKEKEKEKKEKEKQKKKEQAARKK 114

 Score = 26.6 bits (57), Expect = 0.005
 Identities = 34/195 (17%), Positives = 69/195 (35%), Gaps = 27/195 (13%)

Query: 163 ISNKDKEHVSHQNEHNINNVNLKKE------KEYTDIQRDKRKHKRSLSQKSDSYKKRPF 216
           +  +++E +  + E  + N    KE      KE    +R+KRK +  L  +    +K+  
Sbjct: 189 LEREEEERLEKEEEERLANEEKMKEEAKAAKKEKEKAKREKRKAEGKLLTRKQKEEKKLL 248

Query: 217 NKRKTSIERS-------LSNKRYDEKTNKRFNKNFRTDIYNRKHSKDIGYKHNNSYSSSD 269
            +R+ ++  S       L+ K  +E   K+        +Y++K  +    + N S +   
Sbjct: 249 ERRRAALLSSGNVKVAGLAKKDGEENKPKKV-------VYSKKKKRTT--QENASEAIKS 299

Query: 270 DKYHNGRSLSSSDISYQHXXXXXXXXXXXXHDDSSSEISSHTYASRYDKERKRFRKRDIN 329
           D   +   +   ++                 DD     +  T  S    E +     D N
Sbjct: 300 DSKKDSEVVPDDELKESEDVLIDDWENLALGDDDEEGTNEETQESTASHENE-----DQN 354

Query: 330 LERHKERGYINRDKE 344
               +E G    ++E
Sbjct: 355 QGEEEEEGEEEEEEE 369

 Score = 20.4 bits (41), Expect = 0.39
 Identities = 19/114 (16%), Positives = 48/114 (42%), Gaps = 14/114 (12%)

Query: 301 DDSSSEISSHT---------YASRYDKERKRFRKRDINLERH-----KERGYINRDKEGK 346
           DD+S E S+           + S   + +K+  K+ I  ++      K +    ++K+ K
Sbjct: 42  DDTSREASASAEGAEAIEGDFMSTLKQSKKKQEKKVIEEKKDGKPILKSKKEKEKEKKEK 101

Query: 347 HIRRDKEGKHIRRDKEEKHIRRDKEGKHIRGEQERRHQIRNKEKYRDRNDHKKP 400
             ++ KE    ++ +++    ++KE      E+    +   ++  + + +  KP
Sbjct: 102 EKQKKKEQAARKKAQQQAQKEKNKELNKQNVEKAAAEKAAAEKSQKSKGESDKP 155

 Score = 20.4 bits (41), Expect = 0.39
 Identities = 9/48 (18%), Positives = 25/48 (52%)

Query: 352 KEGKHIRRDKEEKHIRRDKEGKHIRGEQERRHQIRNKEKYRDRNDHKK 399
           +E + + R++EE+  + ++E      + +   +   KEK + + + +K
Sbjct: 184 EEQEKLEREEEERLEKEEEERLANEEKMKEEAKAAKKEKEKAKREKRK 231

 Score = 20.0 bits (40), Expect = 0.51
 Identities = 11/23 (47%), Positives = 15/23 (65%), Gaps = 3/23 (13%)

Query: 73 ISQLKQSKEKKDGEEDKYLNAKK 95
          +S LKQSK+K   +E K +  KK
Sbjct: 63 MSTLKQSKKK---QEKKVIEEKK 82

 Score = 19.6 bits (39), Expect = 0.66
 Identities = 13/46 (28%), Positives = 29/46 (63%), Gaps = 5/46 (10%)

Query: 348 IRRDKEGKHIRRDKEEKHIRRDKEGKHIRGEQERRHQIRNKEKYRD 393
           +RR  E K  ++ +E++ + R++E + +  E+E R  + N+EK ++
Sbjct: 173 LRRQLELK--KQLEEQEKLEREEE-ERLEKEEEER--LANEEKMKE 213

 Score = 19.2 bits (38), Expect = 0.86
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 82  KKDGEEDK 89
           KKDGEE+K
Sbjct: 268 KKDGEENK 275

 Score = 16.9 bits (32), Expect = 4.3
 Identities = 6/20 (30%), Positives = 14/20 (70%)

Query: 566 KRKEIDNIMRKKSYRTSKKK 585
           K+K+ +   RKK+ + ++K+
Sbjct: 103 KQKKKEQAARKKAQQQAQKE 122

 Score = 16.5 bits (31), Expect = 5.6
 Identities = 7/22 (31%), Positives = 16/22 (72%), Gaps = 1/22 (4%)

Query: 77  KQSKEKKDGEEDKYL-NAKKLK 97
           ++ +E+ + EE++ L N +K+K
Sbjct: 191 REEEERLEKEEEERLANEEKMK 212

 Score = 15.8 bits (29), Expect = 9.6
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 21  KEKKLIEK 28
           +EKKL+E+
Sbjct: 243 EEKKLLER 250

Lambda     K      H
   0.312    0.130    0.370 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1350
Number of extensions: 98
Number of successful extensions: 32
Number of sequences better than 10.0: 1
Number of HSP's gapped: 17
Number of HSP's successfully gapped: 15
Length of query: 600
Length of database: 1002
Length adjustment: 41
Effective length of query: 559
Effective length of database: 961
Effective search space:   537199
Effective search space used:   537199
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 29 (16.0 bits)
S2: 29 (15.8 bits)