>YPL153C RAD53 SGDID:S000006074, Chr XVI from 264191-261726, reverse
           complement, Verified ORF, "Protein kinase, required for
           cell-cycle arrest in response to DNA damage; activated
           by trans autophosphorylation when interacting with
           hyperphosphorylated Rad9p"
          Length = 821

 Score =  203 bits (517), Expect = 2e-56
 Identities = 112/299 (37%), Positives = 173/299 (57%), Gaps = 16/299 (5%)

Query: 43  NTSCISREGTTTLERKNLILCHSGKLEDKYIIDEKLGQGTYGCVYKGIDKVTNQLYAIKE 102
           NTS I+  G T+    +++   +G  +D  IIDE +GQG +  V K I++ T + +A+K 
Sbjct: 169 NTSKIASPGLTSSTASSMVANKTGIFKDFSIIDEVVGQGAFATVKKAIERTTGKTFAVKI 228

Query: 103 -EKKDRLKNINRFFQEIEIMKKLDHPNIVKLYETYENDNYIYLIMELCSGRELFDSIIEN 161
             K+  + N++   +E+E+++KL+HP IV+L   YE+    Y++ME  SG +L D +  +
Sbjct: 229 ISKRKVIGNMDGVTRELEVLQKLNHPRIVRLKGFYEDTESYYMVMEFVSGGDLMDFVAAH 288

Query: 162 GSFTEKNAATIMKQIFSAIFYLHSLNIVHRDLKPENFLFQSENKDSLLKIIDFGLSKNLG 221
           G+  E     I +QI +AI Y+HS+ I HRDLKP+N L + ++   L+KI DFGL+K  G
Sbjct: 289 GAVGEDAGREISRQILTAIKYIHSMGISHRDLKPDNILIEQDD-PVLVKITDFGLAKVQG 347

Query: 222 TGEFTTTKAGTPYYVAPQVLDGK--------------YDKKCDIWSSGVIMYTLLCGYPP 267
            G F  T  GT  YVAP+V+ GK              Y    D+WS G ++Y +L G+ P
Sbjct: 348 NGSFMKTFCGTLAYVAPEVIRGKDTSVSPDEYEERNEYSSLVDMWSMGCLVYVILTGHLP 407

Query: 268 FYGDTDNEVLKKVKKGEFCFYENDWGSISSDAKNLITKLLTYNPNERCTIEEALNHPWI 326
           F G T +++ K++ +G +         IS +A++ I  LL  +PN R T  +ALNHPWI
Sbjct: 408 FSGSTQDQLYKQIGRGSYHEGPLKDFRISEEARDFIDSLLQVDPNNRSTAAKALNHPWI 466

 Score = 19.6 bits (39), Expect = 0.45
 Identities = 12/22 (54%), Positives = 12/22 (54%), Gaps = 1/22 (4%)

Query: 485 IDSLLQEVDLNGDGEVNKNYNH 506
           IDSLLQ VD N      K  NH
Sbjct: 443 IDSLLQ-VDPNNRSTAAKALNH 463

 Score = 16.9 bits (32), Expect = 2.9
 Identities = 6/15 (40%), Positives = 9/15 (60%)

Query: 161 NGSFTEKNAATIMKQ 175
           NG   EKN+  ++ Q
Sbjct: 112 NGQKVEKNSNQLLSQ 126

 Score = 15.8 bits (29), Expect = 6.6
 Identities = 7/20 (35%), Positives = 12/20 (60%)

Query: 477 ENPLIDKAIDSLLQEVDLNG 496
           ++P++ K  D  L +V  NG
Sbjct: 330 DDPVLVKITDFGLAKVQGNG 349

Lambda     K      H
   0.317    0.137    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 885
Number of extensions: 39
Number of successful extensions: 9
Number of sequences better than 10.0: 1
Number of HSP's gapped: 4
Number of HSP's successfully gapped: 4
Length of query: 509
Length of database: 821
Length adjustment: 38
Effective length of query: 471
Effective length of database: 783
Effective search space:   368793
Effective search space used:   368793
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)