>YDR496C PUF6 SGDID:S000002904, Chr IV from 1443394-1441424, reverse
           complement, Verified ORF, "Pumilio-homology domain
           protein that binds ASH1 mRNA at PUF consensus sequences
           in the 3' UTR and represses its translation, resulting
           in proper asymmetric localization of ASH1 mRNA"
          Length = 656

 Score = 41.2 bits (95), Expect = 2e-07
 Identities = 49/227 (21%), Positives = 98/227 (43%), Gaps = 17/227 (7%)

Query: 315 EIFKKPTKEMFESFSEQNQKALKKYMIEFIENIVEKEMLYNMVSHNIILVACEILDEEEL 374
           E  K  T E     S + +  + + +I  I   VEK      + H  +    +I +E+E+
Sbjct: 276 ETHKDLTIEKVCESSIEKRNIIARNLIGTITASVEKGSTGFQILHAAMREYVKIANEKEI 335

Query: 375 INLMDIIHEGCEYLITTYIGNNALIYLLGYSXXXXXXXXXXXXXXXXXXLCKNSVNFLLI 434
             +++++HE    L+ T  G++    L+  +                  L KN    ++ 
Sbjct: 336 SEMIELLHEQFAELVHTPEGSDVACTLVARANAKERKLILKALKNHAEKLIKNEYGNIVF 395

Query: 435 IRLLKITDDTKILNEFIVKKITANLEDILNDYYG----FYVIL----EFFYNIEQMDKDK 486
           I +L   DDT ++ +     +  +L++ + D +G     Y++L    ++F  I    K++
Sbjct: 396 ITILNCVDDTVLVFKTFSPTVKEHLQEFIIDKFGRRPWLYILLGLDGKYFSPIV---KNE 452

Query: 487 FLYVDWKHLIYSKAPKSVKDGEKRKNEIIKPVIDQ-LQLIFKDRNKL 532
            L    +++  SKA  S KD  +R++E++       L  I KD + +
Sbjct: 453 LL----RYIELSKA-TSKKDPLQRRHELLSKFAPMFLSTISKDYSSI 494

 Score = 26.2 bits (56), Expect = 0.006
 Identities = 23/102 (22%), Positives = 40/102 (39%), Gaps = 16/102 (15%)

Query: 681 PYVKNIISLFIKIFMSTNNTNLAHKIIDNNNNKLYKKITDLFLLNLQIILKSDFVKLFNC 740
           P VKN +  +I++  +T+  +   +      ++L  K   +FL  +     S   +   C
Sbjct: 447 PIVKNELLRYIELSKATSKKDPLQR-----RHELLSKFAPMFLSTISKDYSSILTENLGC 501

Query: 741 -----------LILFLKENDHNIYEQVISTARSLNNNDIYEE 771
                      L   L E D   Y+QV++   +    DI EE
Sbjct: 502 QFIAEVLINDELYAQLNEKDQEKYQQVLNNILTTFKGDITEE 543

 Score = 19.6 bits (39), Expect = 0.57
 Identities = 14/48 (29%), Positives = 21/48 (43%), Gaps = 6/48 (12%)

Query: 195 DIDKFARTNLGYHIITSLIIYGNKDEIY---NKLFKYFYDKCFNDICT 239
           D       NLG   I  ++I    DE+Y   N+  +  Y +  N+I T
Sbjct: 490 DYSSILTENLGCQFIAEVLI---NDELYAQLNEKDQEKYQQVLNNILT 534

 Score = 16.5 bits (31), Expect = 4.8
 Identities = 7/20 (35%), Positives = 13/20 (65%)

Query: 167 LNDLLKSKNDKEEKKRQVGI 186
           L+DL  S ++ EE+  ++ I
Sbjct: 57  LDDLSTSDSEAEEEADELDI 76

 Score = 16.5 bits (31), Expect = 4.8
 Identities = 8/23 (34%), Positives = 10/23 (43%)

Query: 492 WKHLIYSKAPKSVKDGEKRKNEI 514
           W+ L     P   +  EK  NEI
Sbjct: 131 WERLRVKTPPLPKQIREKLSNEI 153

 Score = 16.5 bits (31), Expect = 4.8
 Identities = 7/23 (30%), Positives = 15/23 (65%), Gaps = 1/23 (4%)

Query: 326 ESFSEQNQKALKKYMIEFIENIV 348
           E +++ N+K  +KY  + + NI+
Sbjct: 512 ELYAQLNEKDQEKYQ-QVLNNIL 533

 Score = 16.2 bits (30), Expect = 6.3
 Identities = 6/17 (35%), Positives = 9/17 (52%)

Query: 245 LIFQCFYNHGNDDMKND 261
           LI +   NH    +KN+
Sbjct: 373 LILKALKNHAEKLIKNE 389

 Score = 15.8 bits (29), Expect = 8.2
 Identities = 7/40 (17%), Positives = 18/40 (45%)

Query: 683 VKNIISLFIKIFMSTNNTNLAHKIIDNNNNKLYKKITDLF 722
           +K+  S  ++  +  ++ +   +I+D    K Y   T  +
Sbjct: 166 LKHDASRIVQTLVKYSSKDRREQIVDALKGKFYVLATSAY 205

 Score = 15.8 bits (29), Expect = 8.2
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 274 LSKFGCRLW 282
           + KFG R W
Sbjct: 425 IDKFGRRPW 433

 Score = 15.8 bits (29), Expect = 8.2
 Identities = 5/10 (50%), Positives = 8/10 (80%)

Query: 124 QIKYKNILND 133
           Q KY+ +LN+
Sbjct: 522 QEKYQQVLNN 531

Lambda     K      H
   0.322    0.141    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1066
Number of extensions: 70
Number of successful extensions: 11
Number of sequences better than 10.0: 1
Number of HSP's gapped: 10
Number of HSP's successfully gapped: 10
Length of query: 793
Length of database: 656
Length adjustment: 40
Effective length of query: 753
Effective length of database: 616
Effective search space:   463848
Effective search space used:   463848
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 29 (16.3 bits)
S2: 29 (15.8 bits)