Query= PFI0685w Pf
Annotation|Plasmodium_falciparum_Sanger|(protein coding)
pseudouridylate synthase, putative
(955 letters)
>YOL066C RIB2 SGDID:S000005427, Chr XV from 204471-202696, reverse
complement, Verified ORF, "DRAP deaminase, catalyzes the
third step of the riboflavin biosynthesis pathway;
cytoplasmic tRNA pseudouridine synthase involved in
pseudouridylation of cytoplasmic tRNAs at position 32"
Length = 591
Score = 106 bits (264), Expect = 5e-27
Identities = 52/140 (37%), Positives = 84/140 (60%), Gaps = 2/140 (1%)
Query: 37 KKFEPKYFVK--NKLRFVSPYVYTYKLFSKKRWVGKKIADVMTSEFCAYDMNYFIESIKK 94
KK +P Y V LR + PY +TYK F K+RW +K+ DV SEF + +Y+ ++I +
Sbjct: 64 KKTDPDYEVTIDGPLRKIEPYFFTYKTFCKERWRDRKLVDVFVSEFRDREPSYYSKTIAE 123
Query: 95 GYVKVNKQMVSTDYIIQSNDFIEHKLLLFEKPVLCNKIIILYEDQNFICVYKHSSLPTHP 154
G V +N + + D II+ D I HK+ E PV I I++ED++ + + K SS+P HP
Sbjct: 124 GKVYLNDEPANLDTIIRDGDLITHKVHRHEPPVTSKPIDIVFEDEDILVIDKPSSIPVHP 183
Query: 155 VGSYQYNSLLRILQNYISTS 174
G Y++N++ ++L+ + S
Sbjct: 184 TGRYRFNTITKMLERQLGYS 203
Score = 53.1 bits (126), Expect = 5e-11
Identities = 33/108 (30%), Positives = 54/108 (50%), Gaps = 4/108 (3%)
Query: 681 DKYGDNHNNEDMNNLHIYHKYFIIDFGYIYCENKKLLKYVFTKYTKENGLQFNDYLLKPS 740
D+ GD ++ ++ G + E K ++ V K + +D K +
Sbjct: 228 DRMGDQMKAREVTKEYVARVKGEFPIGIV--EVDKPVRSVNPKVALNAVCEMSDENAKHA 285
Query: 741 ITKFMFLSYNSSIDESLILCQPITGRTHQIRAHLKSLSYPISNDAHYN 788
T F +SY+ S++ C+P+TGRTHQIR HL+ L +PI+ND Y+
Sbjct: 286 KTVFQRVSYDGQT--SIVKCKPLTGRTHQIRVHLQYLGFPIANDPIYS 331
Score = 39.3 bits (90), Expect = 8e-07
Identities = 20/74 (27%), Positives = 38/74 (51%), Gaps = 3/74 (4%)
Query: 489 NVPKYNSYNNHFDKDLKTQDKSYIYTLH---RLDKLTSGIVFFGKNKKFSTYFSQNLSDN 545
++P + + F+ K ++ Y++H RLDK TSG++F K + +
Sbjct: 178 SIPVHPTGRYRFNTITKMLERQLGYSVHPCNRLDKPTSGLMFLAKTPLGADRMGDQMKAR 237
Query: 546 KIKKTYITRVQGDF 559
++ K Y+ RV+G+F
Sbjct: 238 EVTKEYVARVKGEF 251
Score = 19.2 bits (38), Expect = 0.81
Identities = 10/29 (34%), Positives = 16/29 (55%), Gaps = 4/29 (13%)
Query: 333 DKEQTYYHKNVTQN---IND-PHRKDEIL 357
D+E +YY K + + +ND P D I+
Sbjct: 111 DREPSYYSKTIAEGKVYLNDEPANLDTII 139
Score = 18.5 bits (36), Expect = 1.4
Identities = 15/74 (20%), Positives = 31/74 (41%), Gaps = 16/74 (21%)
Query: 873 NTSFNWLYDQNIN---LNQEYNEKDMNKYFFKNINNVSYHSSGIFLHSFRY--------T 921
N + +W++ + L ++ E + Y N++ ++LH+FRY T
Sbjct: 362 NPAESWIHPHSEGEYLLGRQCEECEAEMYTDPGTNDLD-----LWLHAFRYESLERNSDT 416
Query: 922 WTNIFDVFTLLPKW 935
++ T P+W
Sbjct: 417 QKPLWSYRTKYPEW 430
Score = 18.1 bits (35), Expect = 1.8
Identities = 7/17 (41%), Positives = 10/17 (58%)
Query: 558 DFRKLINKLLHSNNIIK 574
DF L+NK + S +K
Sbjct: 11 DFNNLLNKEIESAKEVK 27
Score = 17.3 bits (33), Expect = 3.1
Identities = 10/40 (25%), Positives = 16/40 (40%), Gaps = 14/40 (35%)
Query: 669 DINNDKYGDINN--------------DKYGDNHNNEDMNN 694
D NN+ D NN K+ + +NN + N+
Sbjct: 3 DSNNEASDDFNNLLNKEIESAKEVKLRKFANRNNNRNENS 42
Score = 16.2 bits (30), Expect = 6.9
Identities = 6/8 (75%), Positives = 6/8 (75%)
Query: 40 EPKYFVKN 47
EP FVKN
Sbjct: 545 EPDTFVKN 552
Lambda K H
0.319 0.136 0.405
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1108
Number of extensions: 53
Number of successful extensions: 8
Number of sequences better than 10.0: 1
Number of HSP's gapped: 8
Number of HSP's successfully gapped: 8
Length of query: 955
Length of database: 591
Length adjustment: 40
Effective length of query: 915
Effective length of database: 551
Effective search space: 504165
Effective search space used: 504165
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 29 (16.2 bits)
S2: 29 (15.8 bits)