Query= PFI0685w Pf Annotation|Plasmodium_falciparum_Sanger|(protein coding) pseudouridylate synthase, putative (955 letters)
>YOL066C RIB2 SGDID:S000005427, Chr XV from 204471-202696, reverse
           complement, Verified ORF, "DRAP deaminase, catalyzes the
           third step of the riboflavin biosynthesis pathway;
           cytoplasmic tRNA pseudouridine synthase involved in
           pseudouridylation of cytoplasmic tRNAs at position 32"
          Length = 591

 Score =  106 bits (264), Expect = 5e-27
 Identities = 52/140 (37%), Positives = 84/140 (60%), Gaps = 2/140 (1%)

Query: 37  KKFEPKYFVK--NKLRFVSPYVYTYKLFSKKRWVGKKIADVMTSEFCAYDMNYFIESIKK 94
           KK +P Y V     LR + PY +TYK F K+RW  +K+ DV  SEF   + +Y+ ++I +
Sbjct: 64  KKTDPDYEVTIDGPLRKIEPYFFTYKTFCKERWRDRKLVDVFVSEFRDREPSYYSKTIAE 123

Query: 95  GYVKVNKQMVSTDYIIQSNDFIEHKLLLFEKPVLCNKIIILYEDQNFICVYKHSSLPTHP 154
           G V +N +  + D II+  D I HK+   E PV    I I++ED++ + + K SS+P HP
Sbjct: 124 GKVYLNDEPANLDTIIRDGDLITHKVHRHEPPVTSKPIDIVFEDEDILVIDKPSSIPVHP 183

Query: 155 VGSYQYNSLLRILQNYISTS 174
            G Y++N++ ++L+  +  S
Sbjct: 184 TGRYRFNTITKMLERQLGYS 203

 Score = 53.1 bits (126), Expect = 5e-11
 Identities = 33/108 (30%), Positives = 54/108 (50%), Gaps = 4/108 (3%)

Query: 681 DKYGDNHNNEDMNNLHIYHKYFIIDFGYIYCENKKLLKYVFTKYTKENGLQFNDYLLKPS 740
           D+ GD     ++   ++         G +  E  K ++ V  K       + +D   K +
Sbjct: 228 DRMGDQMKAREVTKEYVARVKGEFPIGIV--EVDKPVRSVNPKVALNAVCEMSDENAKHA 285

Query: 741 ITKFMFLSYNSSIDESLILCQPITGRTHQIRAHLKSLSYPISNDAHYN 788
            T F  +SY+     S++ C+P+TGRTHQIR HL+ L +PI+ND  Y+
Sbjct: 286 KTVFQRVSYDGQT--SIVKCKPLTGRTHQIRVHLQYLGFPIANDPIYS 331

 Score = 39.3 bits (90), Expect = 8e-07
 Identities = 20/74 (27%), Positives = 38/74 (51%), Gaps = 3/74 (4%)

Query: 489 NVPKYNSYNNHFDKDLKTQDKSYIYTLH---RLDKLTSGIVFFGKNKKFSTYFSQNLSDN 545
           ++P + +    F+   K  ++   Y++H   RLDK TSG++F  K    +      +   
Sbjct: 178 SIPVHPTGRYRFNTITKMLERQLGYSVHPCNRLDKPTSGLMFLAKTPLGADRMGDQMKAR 237

Query: 546 KIKKTYITRVQGDF 559
           ++ K Y+ RV+G+F
Sbjct: 238 EVTKEYVARVKGEF 251

 Score = 19.2 bits (38), Expect = 0.81
 Identities = 10/29 (34%), Positives = 16/29 (55%), Gaps = 4/29 (13%)

Query: 333 DKEQTYYHKNVTQN---IND-PHRKDEIL 357
           D+E +YY K + +    +ND P   D I+
Sbjct: 111 DREPSYYSKTIAEGKVYLNDEPANLDTII 139

 Score = 18.5 bits (36), Expect = 1.4
 Identities = 15/74 (20%), Positives = 31/74 (41%), Gaps = 16/74 (21%)

Query: 873 NTSFNWLYDQNIN---LNQEYNEKDMNKYFFKNINNVSYHSSGIFLHSFRY--------T 921
           N + +W++  +     L ++  E +   Y     N++      ++LH+FRY        T
Sbjct: 362 NPAESWIHPHSEGEYLLGRQCEECEAEMYTDPGTNDLD-----LWLHAFRYESLERNSDT 416

Query: 922 WTNIFDVFTLLPKW 935
              ++   T  P+W
Sbjct: 417 QKPLWSYRTKYPEW 430

 Score = 18.1 bits (35), Expect = 1.8
 Identities = 7/17 (41%), Positives = 10/17 (58%)

Query: 558 DFRKLINKLLHSNNIIK 574
           DF  L+NK + S   +K
Sbjct: 11  DFNNLLNKEIESAKEVK 27

 Score = 17.3 bits (33), Expect = 3.1
 Identities = 10/40 (25%), Positives = 16/40 (40%), Gaps = 14/40 (35%)

Query: 669 DINNDKYGDINN--------------DKYGDNHNNEDMNN 694
           D NN+   D NN               K+ + +NN + N+
Sbjct: 3   DSNNEASDDFNNLLNKEIESAKEVKLRKFANRNNNRNENS 42

 Score = 16.2 bits (30), Expect = 6.9
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 40  EPKYFVKN 47
           EP  FVKN
Sbjct: 545 EPDTFVKN 552
Lambda     K      H
   0.319    0.136    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1108
Number of extensions: 53
Number of successful extensions: 8
Number of sequences better than 10.0: 1
Number of HSP's gapped: 8
Number of HSP's successfully gapped: 8
Length of query: 955
Length of database: 591
Length adjustment: 40
Effective length of query: 915
Effective length of database: 551
Effective search space:   504165
Effective search space used:   504165
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 29 (16.2 bits)
S2: 29 (15.8 bits)