>YMR205C PFK2 SGDID:S000004818, Chr XIII from 674765-671886, reverse
           complement, Verified ORF, "Beta subunit of
           heterooctameric phosphofructokinase involved in
           glycolysis, indispensable for anaerobic growth,
           activated by fructose-2,6-bisphosphate and AMP, mutation
           inhibits glucose induction of cell cycle-related genes"
          Length = 959

 Score = 36.6 bits (83), Expect = 1e-05
 Identities = 38/183 (20%), Positives = 75/183 (40%), Gaps = 25/183 (13%)

Query: 257 KNKDDLIAIENIVAKLKLNGLVIIGGDGSNSNAALMAEYFA-----------------ER 299
           K +  L+  ++++ +  ++ L++ GGDGS + A L    +                  ER
Sbjct: 276 KREGRLLGAQHLI-EAGVDALIVCGGDGSLTGADLFRSEWPSLIEELLKTNRISNEQYER 334

Query: 300 QIPISIIGVPKTIDGDLKSEAIEISFGFDTATRTYSEIIGNLCTDVKTGHNVYHVVRVMG 359
              ++I G   +ID D+ +    I   +    R    I  +        H+   VV VMG
Sbjct: 335 MKHLNICGTVGSIDNDMSTTDATIG-AYSALDRICKAI--DYVEATANSHSRAFVVEVMG 391

Query: 360 RSASHVVLECALQTRPNVVLIGEEVEQLNLSLKDIVKNIVNIILKRKSLNKNYGVILLPE 419
           R+   + L   + T  + + I E+      +  +    + +I+ K +S  K   ++++ E
Sbjct: 392 RNCGWLALLAGIATSADYIFIPEK----PATSSEWQDQMCDIVSKHRSRGKRTTIVVVAE 447

Query: 420 GLI 422
           G I
Sbjct: 448 GAI 450

 Score = 30.4 bits (67), Expect = 9e-04
 Identities = 53/252 (21%), Positives = 89/252 (35%), Gaps = 25/252 (9%)

Query: 153 FLKNLHNLPMLNVKDSNNNESFXXXXXXXXXXXXXXXPAPGGHNVISGIYDYAKRYNEQS 212
           F+++L+N   +N  D +N                   PA G ++ +  +  Y      + 
Sbjct: 561 FIEHLNNFMAINSAD-HNEPKLPKDKRLKIAIVNVGAPAGGINSAVYSMATYCMSQGHRP 619

Query: 213 QVIGFLGGIDGLYSKNYVTITDSMMNRFRNLGGFNML-WSGRGKVK--------NKDDLI 263
             I    G  GL     V          R+L   +ML W  RG  +         + DL 
Sbjct: 620 YAI--YNGWSGLARHESV----------RSLNWKDMLGWQSRGGSEIGTNRVTPEEADLG 667

Query: 264 AIENIVAKLKLNGLVIIGG-DGSNSNAALMAEYFAERQIPISIIGVPKTIDGDLKSEAIE 322
            I     K + +GL+I+GG +   S   L     +     I ++ +P T+  ++     E
Sbjct: 668 MIAYYFQKYEFDGLIIVGGFEAFESLHQLERARESYPAFRIPMVLIPATLSNNV--PGTE 725

Query: 323 ISFGFDTATRTYSEIIGNLCTDVKTGHNVYHVVRVMGRSASHVVLECALQTRPNVVLIGE 382
            S G DTA     E    +     +      VV   G ++ ++    +L     V  + E
Sbjct: 726 YSLGSDTALNALMEYCDVVKQSASSTRGRAFVVDCQGGNSGYLATYASLAVGAQVSYVPE 785

Query: 383 EVEQLNLSLKDI 394
           E   L    +DI
Sbjct: 786 EGISLEQLSEDI 797

 Score = 18.9 bits (37), Expect = 2.6
 Identities = 23/131 (17%), Positives = 55/131 (41%), Gaps = 20/131 (15%)

Query: 921  PEHSLFDKENRNKVCETVTKLQLNGLVMPGSNITITEAALLSEYFLEKKIPTSVVGIPLT 980
            PE      E ++++C+ V+K +  G     + + + E A+ ++              P++
Sbjct: 413  PEKPATSSEWQDQMCDIVSKHRSRG--KRTTIVVVAEGAIAADL------------TPIS 458

Query: 981  GSNNLIHELIETCVGFDSSTKVYASLIGNVLTDAVSMPKYWHFIRLMGRSPSHEVLECAL 1040
             S+  +H+++   +G D+      + +G+V     ++        L G    + VLE   
Sbjct: 459  PSD--VHKVLVDRLGLDTR----ITTLGHVQRGGTAVAYDRILATLQGLEAVNAVLESTP 512

Query: 1041 QTHPNVVIISE 1051
             T   ++ ++E
Sbjct: 513  DTPSPLIAVNE 523

 Score = 17.3 bits (33), Expect = 7.6
 Identities = 6/14 (42%), Positives = 10/14 (71%)

Query: 138 GDEFINKNDYEDVK 151
           G E+I +  +EDV+
Sbjct: 243 GPEYIKEFHWEDVR 256

Lambda     K      H
   0.318    0.136    0.399 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 2659
Number of extensions: 111
Number of successful extensions: 5
Number of sequences better than 10.0: 1
Number of HSP's gapped: 4
Number of HSP's successfully gapped: 4
Length of query: 1418
Length of database: 959
Length adjustment: 47
Effective length of query: 1371
Effective length of database: 912
Effective search space:  1250352
Effective search space used:  1250352
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 32 (17.5 bits)
S2: 32 (16.9 bits)