>YDL058W USO1 SGDID:S000002216, Chr IV from 345665-351037, Verified
            ORF, "Essential protein involved in intracellular protein
            transport, coiled-coil protein necessary for transport
            from ER to Golgi; required for assembly of the
            ER-to-Golgi SNARE complex"
          Length = 1790

 Score = 29.3 bits (64), Expect = 5e-04
 Identities = 21/76 (27%), Positives = 39/76 (51%), Gaps = 13/76 (17%)

Query: 81   DYATQENKSFE------EKHLEDLERSNSDIYSESQ----KFDNASDKLETGTQL---TL 127
            D  +QE ++F+      EK++E L+++ SD+    +    K D++ D+ E+   L    L
Sbjct: 1004 DSMSQEKENFQIERGSIEKNIEQLKKTISDLEQTKEEIISKSDSSKDEYESQISLLKEKL 1063

Query: 128  STEATGAVQQITKLSE 143
             T  T   + + K+SE
Sbjct: 1064 ETATTANDENVNKISE 1079

 Score = 27.3 bits (59), Expect = 0.002
 Identities = 20/61 (32%), Positives = 32/61 (52%), Gaps = 1/61 (1%)

Query: 83   ATQENKSFEEKHLEDLERSNSDIYSESQKFDNASDKLETGTQLTLSTEATGAVQQITKLS 142
            A QE+K+  E+ L+ LE  +S   +E +K      KLE+ T  +  TE   +++ I K  
Sbjct: 1474 AAQESKAKVEEGLKKLEEESSKEKAELEKSKEMMKKLES-TIESNETELKSSMETIRKSD 1532

Query: 143  E 143
            E
Sbjct: 1533 E 1533

 Score = 23.5 bits (49), Expect = 0.025
 Identities = 19/68 (27%), Positives = 38/68 (55%), Gaps = 5/68 (7%)

Query: 85   QENKSFEEKHLEDLE---RSNSDIYSESQKFDNASDKLETGTQLTLSTEATGAVQQITKL 141
            Q+  +F+EK + +LE   +++ D  S+  +    S+K++       +TE    +++IT L
Sbjct: 1264 QDECNFKEKEVSELEDKLKASEDKNSKYLELQKESEKIKEELD-AKTTELKIQLEKITNL 1322

Query: 142  SEPAHEES 149
            S+ A E+S
Sbjct: 1323 SK-AKEKS 1329

 Score = 21.9 bits (45), Expect = 0.073
 Identities = 24/91 (26%), Positives = 36/91 (39%), Gaps = 8/91 (8%)

Query: 85   QENKSFEEKHLEDLERSNSDIYSESQKFDNASDKLETGTQLTLSTEATGAVQQITKLSEP 144
            +EN   + K LED+ER   D  +E  K +    +L T     L  E     Q+  K  E 
Sbjct: 1603 EENTVLKSK-LEDIERELKDKQAEI-KSNQEEKELLTSRLKELEQELDSTQQKAQKSEEE 1660

Query: 145  AHEESIYFTYRSVTPCDMNKLDETAKVFSRR 175
               E   F        + ++LDE A +   +
Sbjct: 1661 RRAEVRKF------QVEKSQLDEKAMLLETK 1685

 Score = 20.0 bits (40), Expect = 0.28
 Identities = 14/46 (30%), Positives = 22/46 (47%), Gaps = 5/46 (10%)

Query: 84  TQENKSFEEKHLEDLERSNSDIYSE----SQKFDNASDKLETGTQL 125
           T E+K  +EK+ + L  S+S +         +  N  D L+  TQL
Sbjct: 768 TNEHKELDEKY-QILNSSHSSLKENFSILETELKNVRDSLDEMTQL 812

 Score = 19.6 bits (39), Expect = 0.36
 Identities = 21/112 (18%), Positives = 45/112 (40%), Gaps = 23/112 (20%)

Query: 95   LEDLERSNSDIYSESQKFDNASDKLETGTQ--------------------LTLSTEATGA 134
            + +L ++  ++ +E   + N  ++LET  +                    + L  EAT  
Sbjct: 1077 ISELTKTREELEAELAAYKNLKNELETKLETSEKALKEVKENEEHLKEEKIQLEKEATET 1136

Query: 135  VQQITKLSEPAHEESIYFTYRSVTPCDMNKLDETAKVFSRRCGCDLGERHDE 186
             QQ+  L   A+ ES+   +  +    + K +E      R+   ++ + +DE
Sbjct: 1137 KQQLNSLR--ANLESLEKEHEDLA-AQLKKYEEQIANKERQYNEEISQLNDE 1185

 Score = 19.2 bits (38), Expect = 0.47
 Identities = 18/92 (19%), Positives = 37/92 (40%), Gaps = 4/92 (4%)

Query: 83   ATQENKSFEEKHLEDLERSNSDIYSESQKFDNASDKLETGTQLTLSTEATGAVQQITKLS 142
            + QE K      L++LE+       ++QK +          Q+  S     A+   TK +
Sbjct: 1628 SNQEEKELLTSRLKELEQELDSTQQKAQKSEEERRAEVRKFQVEKSQLDEKAMLLETKYN 1687

Query: 143  EPAHEESIY----FTYRSVTPCDMNKLDETAK 170
            +  ++E  +     T +  T     ++++ AK
Sbjct: 1688 DLVNKEQAWKRDEDTVKKTTDSQRQEIEKLAK 1719

 Score = 18.5 bits (36), Expect = 0.81
 Identities = 11/35 (31%), Positives = 16/35 (45%), Gaps = 1/35 (2%)

Query: 118 KLETGTQLTLSTEATGAVQQITKLSEPAHEESIYF 152
           K  T  Q TL  E     +    LSEP  ++ ++F
Sbjct: 260 KYNTSNQ-TLFLETGNLPKLAHLLSEPISQDEVFF 293

 Score = 17.7 bits (34), Expect = 1.4
 Identities = 9/34 (26%), Positives = 19/34 (55%)

Query: 86   ENKSFEEKHLEDLERSNSDIYSESQKFDNASDKL 119
            +N++FE++     E S++     S+K +   D+L
Sbjct: 1361 KNQAFEKERKLLNEGSSTITQEYSEKINTLEDEL 1394

 Score = 16.2 bits (30), Expect = 4.0
 Identities = 4/11 (36%), Positives = 9/11 (81%)

Query: 10 VKEPKRKDIDE 20
          +++PK + +DE
Sbjct: 8  IQQPKIQSVDE 18

 Score = 16.2 bits (30), Expect = 4.0
 Identities = 9/35 (25%), Positives = 16/35 (45%)

Query: 86   ENKSFEEKHLEDLERSNSDIYSESQKFDNASDKLE 120
            EN+S  +   E    S+  + +   K D+ S + E
Sbjct: 977  ENESLIKAVEESKNESSIQLSNLQNKIDSMSQEKE 1011

 Score = 15.8 bits (29), Expect = 5.2
 Identities = 8/35 (22%), Positives = 15/35 (42%)

Query: 91   EEKHLEDLERSNSDIYSESQKFDNASDKLETGTQL 125
            +EK   +L R       E +  +   +KL+   Q+
Sbjct: 1326 KEKSESELSRLKKTSSEERKNAEEQLEKLKNEIQI 1360

 Score = 15.8 bits (29), Expect = 5.2
 Identities = 7/17 (41%), Positives = 12/17 (70%), Gaps = 1/17 (5%)

Query: 8    SKVKEPKRKDIDELAER 24
            S++ E + KDI+EL  +
Sbjct: 1558 SRINESE-KDIEELKSK 1573

 Score = 15.4 bits (28), Expect = 6.8
 Identities = 8/32 (25%), Positives = 16/32 (50%)

Query: 83   ATQENKSFEEKHLEDLERSNSDIYSESQKFDN 114
            +TQ+     +K  ++LE     + S S++  N
Sbjct: 1188 STQQENESIKKKNDELEGEVKAMKSTSEEQSN 1219

Lambda     K      H
   0.310    0.126    0.353 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 720
Number of extensions: 33
Number of successful extensions: 15
Number of sequences better than 10.0: 1
Number of HSP's gapped: 14
Number of HSP's successfully gapped: 14
Length of query: 204
Length of database: 1790
Length adjustment: 36
Effective length of query: 168
Effective length of database: 1754
Effective search space:   294672
Effective search space used:   294672
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 27 (15.0 bits)
S2: 27 (15.0 bits)