>YDL058W USO1 SGDID:S000002216, Chr IV from 345665-351037, Verified
            ORF, "Essential protein involved in intracellular protein
            transport, coiled-coil protein necessary for transport
            from ER to Golgi; required for assembly of the
            ER-to-Golgi SNARE complex"
          Length = 1790

 Score = 40.8 bits (94), Expect = 7e-07
 Identities = 54/220 (24%), Positives = 89/220 (40%), Gaps = 40/220 (18%)

Query: 463  INLKKQK-ERYTNVINE----IYKEKDEIIKNHVDELAKLSNHYNEVVLVNENIKNEM-- 515
            I +K Q  E+   ++NE    I +E  E I    DEL +L N         +N ++E+  
Sbjct: 1358 IQIKNQAFEKERKLLNEGSSTITQEYSEKINTLEDELIRLQNENELKAKEIDNTRSELEK 1417

Query: 516  -NFLNDEILK-KSCEIEKLQDRLLDYESKXXXXXXXXXXXXXXXXXD------------- 560
             +  NDE+L+ K   I+ LQD +L Y+ K                 D             
Sbjct: 1418 VSLSNDELLEEKQNTIKSLQDEILSYKDKITRNDEKLLSIERDNKRDLESLKEQLRAAQE 1477

Query: 561  -------HLRKLLSKHIHRNEQLLNTTFLLQK--STLENNSLEKKIIELRAKSYRKDQEI 611
                    L+KL  +      +L  +  +++K  ST+E+N  E K      ++ RK  E 
Sbjct: 1478 SKAKVEEGLKKLEEESSKEKAELEKSKEMMKKLESTIESNETELKS---SMETIRKSDE- 1533

Query: 612  KKLQETKSIRKSPSSNCSHNSQEYSMDLIDDLNEDNYNLK 651
             KL+++K   +    N  H       DLI  +NE   +++
Sbjct: 1534 -KLEQSKKSAEEDIKNLQHEKS----DLISRINESEKDIE 1568

 Score = 40.0 bits (92), Expect = 1e-06
 Identities = 24/74 (32%), Positives = 42/74 (56%), Gaps = 11/74 (14%)

Query: 254  NEENMTKDEKLVELKKEIESNYMHLREYKNFQNELEVDVNNKIYSAENYVNRTNQRLIEN 313
            N+EN+ K  +L + ++E+E+    L  YKN +NELE     K+ ++E  +        EN
Sbjct: 1070 NDENVNKISELTKTREELEAE---LAAYKNLKNELE----TKLETSEKALKEVK----EN 1118

Query: 314  NIDIKEKKLNIEKQ 327
               +KE+K+ +EK+
Sbjct: 1119 EEHLKEEKIQLEKE 1132

 Score = 38.9 bits (89), Expect = 2e-06
 Identities = 38/160 (23%), Positives = 74/160 (46%), Gaps = 22/160 (13%)

Query: 460  EYAINLKKQKERYTNVINEIYKEKDEIIKNHVDELAKLSNHYNEVVLVNENIKNEMNFLN 519
            E   NL K KE+  + ++ + K   E  KN  ++L KL N   E+ + N+  + E   LN
Sbjct: 1317 EKITNLSKAKEKSESELSRLKKTSSEERKNAEEQLEKLKN---EIQIKNQAFEKERKLLN 1373

Query: 520  DE----ILKKSCEIEKLQDRLLDYESKXXXXXXXXXXXXXXXXXDHLRKLLSKHIHRNEQ 575
            +       + S +I  L+D L+  +++                 D+ R  L K    N++
Sbjct: 1374 EGSSTITQEYSEKINTLEDELIRLQNE---------NELKAKEIDNTRSELEKVSLSNDE 1424

Query: 576  LLNTTFLLQKSTLENNSLEKKIIELRAKSYRKDQEIKKLQ 615
            LL      +++T++  SL+ +I+  + K  R D+++  ++
Sbjct: 1425 LLEE----KQNTIK--SLQDEILSYKDKITRNDEKLLSIE 1458

 Score = 38.5 bits (88), Expect = 3e-06
 Identities = 49/240 (20%), Positives = 97/240 (40%), Gaps = 8/240 (3%)

Query: 460  EYAINLKKQKERYTNVINEIYKEKDEIIKNHVDELAKLSNHYNEVVLVNENIKNEMNFLN 519
            E  I L+K+       +N +    + + K H D  A+L  +  ++         E++ LN
Sbjct: 1124 EEKIQLEKEATETKQQLNSLRANLESLEKEHEDLAAQLKKYEEQIANKERQYNEEISQLN 1183

Query: 520  DEILKKSCEIEKLQDR--LLDYESKXXXXXXXXXXXXXXXXXDHLRKLLSKHIHRNEQLL 577
            DEI     E E ++ +   L+ E K                 D L  L  K + +  +  
Sbjct: 1184 DEITSTQQENESIKKKNDELEGEVKAMKSTSEEQSNLKKSEIDAL-NLQIKELKKKNETN 1242

Query: 578  NTTFLLQKSTLENNSLEKKIIELRAKSYRKDQEIKKLQETKSIRKSPSSNCSH-NSQEYS 636
              + L    ++E+ ++  KI EL+ +   K++E+ +L++   ++ S   N  +   Q+ S
Sbjct: 1243 EASLLESIKSVESETV--KIKELQDECNFKEKEVSELED--KLKASEDKNSKYLELQKES 1298

Query: 637  MDLIDDLNEDNYNLKYYHNNSIVGDQENSVYTSKKSYDKNITIGSLADTEKRSIKIKNII 696
              + ++L+     LK          +      S+ S  K  +     + E++  K+KN I
Sbjct: 1299 EKIKEELDAKTTELKIQLEKITNLSKAKEKSESELSRLKKTSSEERKNAEEQLEKLKNEI 1358

 Score = 35.8 bits (81), Expect = 2e-05
 Identities = 60/331 (18%), Positives = 129/331 (38%), Gaps = 40/331 (12%)

Query: 7    SEDSNISKKYVYGEDLENIKNDNSTTNDKINDE-ENIIDMKKETNENISHLNYNEACPNS 65
            S + NI +      DLE  K +  + +D   DE E+ I + KE  E  +  N       S
Sbjct: 1019 SIEKNIEQLKKTISDLEQTKEEIISKSDSSKDEYESQISLLKEKLETATTANDENVNKIS 1078

Query: 66   IIEKGHDVITHNILEEKNDQCNTNIILPIVKECNDSHKDISLXXXXXXXXXXXXXXYQHD 125
             + K  + +   +   KN +   N +   ++    + K++                 + +
Sbjct: 1079 ELTKTREELEAELAAYKNLK---NELETKLETSEKALKEV-----------------KEN 1118

Query: 126  KDHISNFNLDEVESSAQDETNEAKSDDSKMKIKIQNLLKLIGLLKAQINQKDQEIYKMEV 185
            ++H     L E +   + E  E K   + ++  +++L K    L AQ+ + +++I   E 
Sbjct: 1119 EEH-----LKEEKIQLEKEATETKQQLNSLRANLESLEKEHEDLAAQLKKYEEQIANKER 1173

Query: 186  DFKLRXXXXXXXXXXXXXXXXXXXSHTERTEGDNFMMSKMKSILISQNEEIAXXXXXXXX 245
             +                         +  EG+      +K++  +  E+          
Sbjct: 1174 QYNEEISQLNDEITSTQQENESIKKKNDELEGE------VKAMKSTSEEQSNLKKSEIDA 1227

Query: 246  XXXXIFYLNEENMTKDEKLVELKKEIESNYMHLREYK---NFQNELEVDVNNKIYSAEN- 301
                I  L ++N T +  L+E  K +ES  + ++E +   NF+ +   ++ +K+ ++E+ 
Sbjct: 1228 LNLQIKELKKKNETNEASLLESIKSVESETVKIKELQDECNFKEKEVSELEDKLKASEDK 1287

Query: 302  ---YVN-RTNQRLIENNIDIKEKKLNIEKQK 328
               Y+  +     I+  +D K  +L I+ +K
Sbjct: 1288 NSKYLELQKESEKIKEELDAKTTELKIQLEK 1318

 Score = 34.3 bits (77), Expect = 6e-05
 Identities = 64/362 (17%), Positives = 136/362 (37%), Gaps = 45/362 (12%)

Query: 6    KSEDSNISKKYVYGEDLENIKNDNSTTND------KINDE-ENIIDMKKETNENISHLNY 58
            K E+   +K+  Y E++  + ++ ++T        K NDE E  +   K T+E  S+L  
Sbjct: 1163 KYEEQIANKERQYNEEISQLNDEITSTQQENESIKKKNDELEGEVKAMKSTSEEQSNLKK 1222

Query: 59   NEACPNSI----IEKGHDVITHNILEE-KNDQCNTNIILPIVKECNDSHKDISLXXXXXX 113
            +E    ++    ++K ++    ++LE  K+ +  T  I  +  ECN   K++S       
Sbjct: 1223 SEIDALNLQIKELKKKNETNEASLLESIKSVESETVKIKELQDECNFKEKEVSELEDKLK 1282

Query: 114  XXXXXXXXY---QHDKDHISN----------------FNLDEVESSAQDETNEAKSDDSK 154
                    Y   Q + + I                   NL + +  ++ E +  K   S+
Sbjct: 1283 ASEDKNSKYLELQKESEKIKEELDAKTTELKIQLEKITNLSKAKEKSESELSRLKKTSSE 1342

Query: 155  MKIKIQNLLKLIGLLKAQINQKDQEIYKMEVDFKLRXXXXXXXXXXXXXXXXXXXSHTER 214
             +   +N  + +  LK +I  K+Q   K   + KL                        R
Sbjct: 1343 ER---KNAEEQLEKLKNEIQIKNQAFEK---ERKLLNEGSSTITQEYSEKINTLEDELIR 1396

Query: 215  TEGDNFMMSK--------MKSILISQNEEIAXXXXXXXXXXXXIFYLNEENMTKDEKLVE 266
             + +N + +K        ++ + +S +E +             I    ++    DEKL+ 
Sbjct: 1397 LQNENELKAKEIDNTRSELEKVSLSNDELLEEKQNTIKSLQDEILSYKDKITRNDEKLLS 1456

Query: 267  LKKEIESNYMHLREYKNFQNELEVDVNNKIYSAENYVNRTNQRLIENNIDIKEKKLNIEK 326
            ++++ + +   L+E      E +  V   +   E   ++    L ++   +K+ +  IE 
Sbjct: 1457 IERDNKRDLESLKEQLRAAQESKAKVEEGLKKLEEESSKEKAELEKSKEMMKKLESTIES 1516

Query: 327  QK 328
             +
Sbjct: 1517 NE 1518

 Score = 33.1 bits (74), Expect = 1e-04
 Identities = 50/213 (23%), Positives = 85/213 (39%), Gaps = 37/213 (17%)

Query: 465  LKKQKERYTNVINEIYKEKDEIIKNHVDELAKLSN----HYNEVVLVNENIKNEMNFLND 520
            L ++ +        + KEKD+   NH  E   L         E+  +NEN++ EM    +
Sbjct: 872  LSREMQAVEENCKNLQKEKDKSNVNHQKETKSLKEDIAAKITEIKAINENLE-EMKIQCN 930

Query: 521  EILKKSCEIEKLQDRLLDYESKXXXXXXXXXXXXXXXXXDHLRKLLSKHIH---RNEQLL 577
             + K   E E +   L++Y+S+                 + L+ L + +      NE L+
Sbjct: 931  NLSK---EKEHISKELVEYKSR-----FQSHDNLVAKLTEKLKSLANNYKDMQAENESLI 982

Query: 578  NTT------FLLQKSTLENN----SLEKKIIELRAKSYRKDQE-----IKKLQETKS--I 620
                       +Q S L+N     S EK+  ++   S  K+ E     I  L++TK   I
Sbjct: 983  KAVEESKNESSIQLSNLQNKIDSMSQEKENFQIERGSIEKNIEQLKKTISDLEQTKEEII 1042

Query: 621  RKSPSSNCSHNSQ----EYSMDLIDDLNEDNYN 649
             KS SS   + SQ    +  ++     N++N N
Sbjct: 1043 SKSDSSKDEYESQISLLKEKLETATTANDENVN 1075

 Score = 29.6 bits (65), Expect = 0.002
 Identities = 118/675 (17%), Positives = 238/675 (35%), Gaps = 113/675 (16%)

Query: 21   DLENIKNDNSTTNDKIND-EENIIDMKKETNENISHLNYNEACPNSIIEKGHDVITHNIL 79
            +LE+++ ++     ++   EE I + +++ NE IS LN          E          +
Sbjct: 1146 NLESLEKEHEDLAAQLKKYEEQIANKERQYNEEISQLNDEITSTQQENES---------I 1196

Query: 80   EEKNDQCNTNIILPIVKECNDSHKDISLXXXXXXXXXXXXXXYQHDKDHISNFNLDEVES 139
            ++KND+    +    +K  ++   ++                    K  I   NL   E 
Sbjct: 1197 KKKNDELEGEV--KAMKSTSEEQSNLK-------------------KSEIDALNLQIKEL 1235

Query: 140  SAQDETNEAKSDDSKMKIKIQNLLKLIGLLKAQINQKDQEIYKMEVDFKLRXXXXXXXXX 199
              ++ETNEA   +S   ++ + +   I  L+ + N K++E+ ++E   K           
Sbjct: 1236 KKKNETNEASLLESIKSVESETVK--IKELQDECNFKEKEVSELEDKLKASEDKNSKYLE 1293

Query: 200  XXXXXXXXXXSHTERTEGDNFMMSKMKSILISQNEEIAXXXXXXXXXXXXIFYLNEENMT 259
                          +T      + K+ ++  ++ +  +                +EE   
Sbjct: 1294 LQKESEKIKEELDAKTTELKIQLEKITNLSKAKEKSESELSRLKKTS-------SEERKN 1346

Query: 260  KDEKLVELKKEIESNYMHLREYKNFQNE----LEVDVNNKIYSAENYVNRTNQRLIENNI 315
             +E+L +LK EI+       + +   NE    +  + + KI + E+ + R     ++N  
Sbjct: 1347 AEEQLEKLKNEIQIKNQAFEKERKLLNEGSSTITQEYSEKINTLEDELIR-----LQNEN 1401

Query: 316  DIKEKKLN-----IEKQKRIIXXXXXXXXXXXXXXXXXXXXXXXXXXNNSRDIIKYKQNN 370
            ++K K+++     +EK                                N   ++  +++N
Sbjct: 1402 ELKAKEIDNTRSELEKVSLSNDELLEEKQNTIKSLQDEILSYKDKITRNDEKLLSIERDN 1461

Query: 371  MDLINRXXXXXXXXXXQKIEIENMHMNYKQXXXXXXXXXXXXXXXHQNLLSKDEENNKMV 430
               +             K ++E      ++                + L S  E N    
Sbjct: 1462 KRDLESLKEQLRAAQESKAKVEEGLKKLEEESSKEKAELEKSKEMMKKLESTIESNE--- 1518

Query: 431  HDINRLKFDMEVRXXXXXXXXXXXXXXXXEYAINLKKQKERYTNVINEIYKEKDEIIKNH 490
                 LK  ME                  E   NL+ +K    + INE  K+ +E +K+ 
Sbjct: 1519 ---TELKSSMET-IRKSDEKLEQSKKSAEEDIKNLQHEKSDLISRINESEKDIEE-LKSK 1573

Query: 491  VDELAKLSNHYNEVVLVNENIKNEMNFLNDEILKKSCEIEKLQDRLLDYESKXXXXXXXX 550
            +   AK  +         E +K E+N   ++I   + E   L+ +L D E +        
Sbjct: 1574 LRIEAKSGSEL-------ETVKQELNNAQEKIRINAEENTVLKSKLEDIERE------LK 1620

Query: 551  XXXXXXXXXDHLRKLLSKHIHRNEQLLNTT-----------------FLLQKSTLENNS- 592
                        ++LL+  +   EQ L++T                 F ++KS L+  + 
Sbjct: 1621 DKQAEIKSNQEEKELLTSRLKELEQELDSTQQKAQKSEEERRAEVRKFQVEKSQLDEKAM 1680

Query: 593  -LEKKIIEL--RAKSYRKD------------QEIKKLQETKSIRKSPSSNCSHNSQEYS- 636
             LE K  +L  + +++++D            QEI+KL +     K+ +S     +++ S 
Sbjct: 1681 LLETKYNDLVNKEQAWKRDEDTVKKTTDSQRQEIEKLAKELDNLKAENSKLKEANEDRSE 1740

Query: 637  ----MDLIDDLNEDN 647
                M L+ DL+E N
Sbjct: 1741 IDDLMLLVTDLDEKN 1755

 Score = 26.9 bits (58), Expect = 0.010
 Identities = 45/247 (18%), Positives = 97/247 (39%), Gaps = 47/247 (19%)

Query: 460  EYAINLKKQKERYTNVINEIYKEKDEIIKNHVDELAKLSNHYNEVVLVNENIKNEMNFLN 519
            E  I+L K+K       N      DE + N + EL K        +   +N+KNE+    
Sbjct: 1053 ESQISLLKEKLETATTAN------DENV-NKISELTKTREELEAELAAYKNLKNELETKL 1105

Query: 520  DEILKKSCEIEKLQDRLLDYESKXXXXXXXXXXXXXXXXXDHLRKLLSKHIHRNEQLLNT 579
            +   K   E+++ ++ L + + +                       L K     +Q LN+
Sbjct: 1106 ETSEKALKEVKENEEHLKEEKIQ-----------------------LEKEATETKQQLNS 1142

Query: 580  TFLLQKSTLENNSLEKKIIELRAKSYRK---------DQEIKKLQE--TKSIRKSPSSNC 628
                 ++ LE+   E + +  + K Y +         ++EI +L +  T + +++ S   
Sbjct: 1143 L----RANLESLEKEHEDLAAQLKKYEEQIANKERQYNEEISQLNDEITSTQQENESIKK 1198

Query: 629  SHNSQEYSMDLIDDLNEDNYNLKYYHNNSIVGDQENSVYTSKKSYDKNITIGSLADTEKR 688
             ++  E  +  +   +E+  NLK    +++  + +      K   ++   + S+   E  
Sbjct: 1199 KNDELEGEVKAMKSTSEEQSNLKKSEIDAL--NLQIKELKKKNETNEASLLESIKSVESE 1256

Query: 689  SIKIKNI 695
            ++KIK +
Sbjct: 1257 TVKIKEL 1263

 Score = 26.2 bits (56), Expect = 0.017
 Identities = 41/191 (21%), Positives = 74/191 (38%), Gaps = 27/191 (14%)

Query: 3    SDTKSEDSNISKKYVYGEDLENIKNDNSTTNDKINDEENIIDMKKETNENISHLNYNEAC 62
            SD K E S  S +    ED++N++++ S    +IN+ E      K+  E  S L   EA 
Sbjct: 1531 SDEKLEQSKKSAE----EDIKNLQHEKSDLISRINESE------KDIEELKSKLRI-EAK 1579

Query: 63   PNSIIEKGHDVITHNILEEKNDQCNTNIIL-----PIVKECNDSHKDISLXXXXXXXXXX 117
              S +E     + +N  E+       N +L      I +E  D   +I            
Sbjct: 1580 SGSELETVKQEL-NNAQEKIRINAEENTVLKSKLEDIERELKDKQAEIKSNQEEKELLTS 1638

Query: 118  XXXXYQHDKDHISNFNLDEVESSAQDETNEAKSDDSKMKIKIQNLLKLIGLLKAQINQ-- 175
                 + +        LD  +  AQ    E +++  K +++   L +   LL+ + N   
Sbjct: 1639 RLKELEQE--------LDSTQQKAQKSEEERRAEVRKFQVEKSQLDEKAMLLETKYNDLV 1690

Query: 176  KDQEIYKMEVD 186
              ++ +K + D
Sbjct: 1691 NKEQAWKRDED 1701

 Score = 23.1 bits (48), Expect = 0.14
 Identities = 11/40 (27%), Positives = 20/40 (50%)

Query: 6    KSEDSNISKKYVYGEDLENIKNDNSTTNDKINDEENIIDM 45
            K+ DS   +     ++L+N+K +NS   +   D   I D+
Sbjct: 1705 KTTDSQRQEIEKLAKELDNLKAENSKLKEANEDRSEIDDL 1744

 Score = 19.6 bits (39), Expect = 1.6
 Identities = 8/25 (32%), Positives = 15/25 (60%)

Query: 736 LNDKKVVISFINGDLYVEDDASPIK 760
           L+DK V+ S ++    ++D+   IK
Sbjct: 596 LSDKSVIKSLLSFSYQIQDEDVTIK 620

Lambda     K      H
   0.310    0.129    0.348 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 3909
Number of extensions: 286
Number of successful extensions: 36
Number of sequences better than 10.0: 1
Number of HSP's gapped: 21
Number of HSP's successfully gapped: 20
Length of query: 771
Length of database: 1790
Length adjustment: 47
Effective length of query: 724
Effective length of database: 1743
Effective search space:  1261932
Effective search space used:  1261932
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 33 (17.7 bits)
S2: 33 (17.3 bits)